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Resistance against Pissodes strobi (Coleoptera: Curculionidae) in severed leaders and in a water-soluble bark extract of Picea sitchensis (Pinaceae): evidence for a post-ingestive mode of action

Published online by Cambridge University Press:  31 May 2012

T.S. Sahota ,
J.F. Manville ,
J. Hollmann ,
I. Leal ,
A. Ibaraki  and
E. White
T.S. Sahota*
Affiliation:
Pacific Forestry Centre, Natural Resources Canada, 506 West Burnside Road, Victoria, British Columbia, Canada V8Z 1M5
J.F. Manville
Affiliation:
Pacific Forestry Centre, Natural Resources Canada, 506 West Burnside Road, Victoria, British Columbia, Canada V8Z 1M5
J. Hollmann
Affiliation:
Pacific Forestry Centre, Natural Resources Canada, 506 West Burnside Road, Victoria, British Columbia, Canada V8Z 1M5
I. Leal
Affiliation:
Pacific Forestry Centre, Natural Resources Canada, 506 West Burnside Road, Victoria, British Columbia, Canada V8Z 1M5
A. Ibaraki
Affiliation:
Pacific Forestry Centre, Natural Resources Canada, 506 West Burnside Road, Victoria, British Columbia, Canada V8Z 1M5
E. White
Affiliation:
Pacific Forestry Centre, Natural Resources Canada, 506 West Burnside Road, Victoria, British Columbia, Canada V8Z 1M5
*
1 Author to whom all correspondence should be addressed.
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Abstract

The levels of ovarian growth and transcription of the vitellogenin gene were compared in Pissodes strobi Peck (Coleoptera: Curculionidae) feeding on severed leaders from susceptible and resistant Picea sitchensis (Bong) Carr (Pinaceae) and in weevils given a dose of an aqueous extract from the bark of such leaders. A force-feeding method was developed to deliver the extracts into the alimentary canal of the weevils. Weevils given one dose of the aqueous extract from resistant leaders, followed by feeding on sections of laterals from susceptible trees, exhibited a 60% inhibition of oocyte growth relative to insects given the extract from susceptible leaders. In similar experiments, transcription of the vitellogenin gene of the weevils was inhibited by about 48%. Transcription of the vitellogenin gene was reduced by about 60% in weevils feeding on the severed leaders from the resistant clone relative to those feeding on severed leaders from susceptible trees. Results indicate that these effects of resistance do not require an intact tree. Experiments using extracts show that the observed effects result from a post-ingestive effect of the extract.

Résumé

Nous avons comparé la croissance ovarienne et la transcription du gène de la vitellogénine chez des Pissodes strobi Peck (Coleoptera : Curculionidae) se nourrissant de pousses terminales coupées de Picea sitchensis (Bong) Carr (Pinaceae), sensibles ou résistantes, et chez des charançons qui avaient reçu une dose d’extrait aqueux d’écorce de ces pousses. Une méthode de gavage a été mise au point pour introduire les extraits d’écorce dans le canal alimentaire des charançons. Les charançons qui avaient reçu une dose d’extrait d’écorce de pousses terminales résistantes et qui avaient ensuite été nourris de sections de pousses terminales latérales sensibles, ont vu la croissance de leurs oocytes diminuer de 60% comparativement aux insectes qui avaient reçu de l’extrait de pousses sensibles. Dans d’autres expériences du même type, la transcription du gène de la vitellogénine de charançon a été inhibée d’environ 48%. La transcription du gène était réduite d’environ 60% dans le cas de charançons nourris de pousses terminales coupées du clone résistant plutôt que de pousses coupées d’arbres sensibles. Les résultats indiquent que ces effets de la résistance se manifestent même si les arbres ne sont pas intacts. Les expériences démontrent que les effets observés résultent de l’action de l’extrait après l’ingestion.

[Traduit par la Rédaction]

Type
Articles
Information
The Canadian Entomologist , Volume 133 , Issue 3 , June 2001 , pp. 315 - 323
Copyright
Copyright © Entomological Society of Canada 2001

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