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DIGESTIVE PROCESSES OF HAEMATOPHAGOUS INSECTS: V. INHIBITORS OF TRYPSIN FROM GLOSSINA MORSITANS MORSITANS (DIPTERA: GLOSSINIDAE)

Published online by Cambridge University Press:  31 May 2012

R. H. Gooding
R. H. Gooding
Affiliation:
Department of Entomology, University of Alberta, Edmonton
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Abstract

Trypsin has been partially purified from the midguts of Glossina morsitans. It is inactivated by tosyl-l-lysine chloromethyl ketone, phenylmethane sulfonylfluoride, and 2-mercaptoethanol. Mammalian sera inhibit G. morsitans trypsin in vitro. Sheep serum contains at least two inhibitors of G. morsitans trypsin and a high molecular weight material which protects against serum inhibition. Very low levels of trypsin inhibitor are found in G. morsitans primary excreta and in the salivary glands, Malpighian tubules, and anterior midguts of unfed flies. High concentrations of trypsin inhibitor are found in the anterior (non-digestive) section of the midgut for at least 48 h after flies fed on goats.

Type
Articles
Information
The Canadian Entomologist , Volume 106 , Issue 1 , January 1974 , pp. 39 - 44
Copyright
Copyright © Entomological Society of Canada 1974

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References

Akov, S. 1972. Protein digestion in haematophagous insects, pp. 531–540. In Rodriguez, J. G. (Ed.), Insect and mite nutrition, xiii + 702 pp. North-Holland Publishing Company, Amsterdam.Google Scholar
Buxton, P. A. 1955. The natural history of tsetse flies. London School of Hygiene and Tropical Medicine Memoir 10. H. R. Lewis and Co. Ltd., London. ix + 816 pp. + 47 plates.Google Scholar
Gooding, R. H. 1972 a. Digestive processes of haematophagous insects. I. A literature review. Quaest. ent. 8: 5–60.Google Scholar
Gooding, R. H. 1972 b. Digestive processes of haematophagous insects. II. Trypsin from the sheep ked Melophagous ovinus (L.) (Hippoboscidae, Diptera) and its inhibition by mammalian sera. Comp. Biochem. Physiol. 43B: 815–824.Google Scholar
Hawkins, R. I. 1966. Factors affecting blood clotting from salivary glands and crop of Glossina austeni. Nature, Lond. 212: 738–739.CrossRefGoogle Scholar
Huang, C-T. 1971 a. Vertebrate serum inhibitors of Aedes aegypti trypsin. Insect Biochem. 1: 27–38.CrossRefGoogle Scholar
Huang, C-T. 1971 b. The interactions of Aedes aegypti (L.) trypsin with its two inhibitors found in bovine serum. Insect Biochem. 1: 207–227.CrossRefGoogle Scholar
Langley, P. A. 1966. The control of digestion in the tsetse fly, Glossina morsitans. Enzyme activity in relation to the size and nature of the meal. J. Insect Physiol. 12: 439–448.CrossRefGoogle Scholar
Layne, E. 1957. Spectrophotometric and turbidometric methods for measuring proteins. In Colowick, S. P. and Kaplan, N. O. (Eds.), Methods in enzymology, Vol. 3, pp. 447–454. Academic Press, New York.Google Scholar
Nash, T. A. M., Jordan, A. M., and Trewern, M. A.. 1971. Mass rearing of tsetse flies (Glossina spp.). In Sterility principle for insect control or eradication, pp. 99–110. I.A.E.A., Vienna.Google Scholar
Yang, Y. J. and Davies, D. M.. 1972. A mosquito-chymotrypsin inhibitor in tissues of adult Aedes aegypti. Comp. Biochem. Physiol. 43B: 137–141.Google Scholar