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Nerve Microvessel Changes in Diabetes are Prevented by Aldose Reductase Inhibition

Published online by Cambridge University Press:  18 September 2015

Timothy J. Benstead  and
Virgilio E. Sangalang
Timothy J. Benstead*
Affiliation:
Department of Medicine, Dalhousie University, Halifax
Virgilio E. Sangalang
Affiliation:
Department of Pathology, Dalhousie University, Halifax
*
Division of Neurology, Department of Medicine. Dalhousie University, Victoria General Hospital, Halifax, Nova Scotia. Canada B3H 2Y9
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Abstract

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Background

Despite the potential importance of endoneurial microvessel abnormalities in diabetic neuropathy, the pathogenesis of these abnormalities is incompletely understood. We wished to evaluate the effect of experimental diabetes on endoneurial microvessels and determine if an aldose reductase inhibitor alters any of the changes induced by diabetes.

Methods

We compared streptozocin diabetic rats with and without aldose reductase inhibitor treatment to non-diabetic rats after 10 months of diabetes. Transverse microvessels from the mid-sciatic level were studied by electron microscopic morphometric evaluation.

Results

Microvessel endothelial, pericyte, basement membrane and total mural area were greater in untreated diabetic animals than non-diabetic animals. Aldose reductase inhibitor treated diabetic animals had greater endothelial area and possibly pericyte area but not basement membrane or total mural area.

Conclusions

This study demonstrates that endoneurial microvessel abnormalities can be detected in experimental diabetic neuropathy. Microvessel basement membrane thickening will be prevented by an aldose reductase inhibitor. One mechanism by which abnormal polyol pathway activity may contribute to diabetic neuropathy could be through damage to microvessels.

Type
Original Articles
Information
Canadian Journal of Neurological Sciences , Volume 22 , Issue 3 , August 1995 , pp. 192 - 197
Copyright
Copyright © Canadian Neurological Sciences Federation 1995

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