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Construction of plant expression vector and analysis of herbicide resistance and salt tolerance of transgenic tobacco

Published online by Cambridge University Press:  12 February 2007

Wang Xian-Yan ,
Shan Xiao-Yi ,
Yang Ai-Fang  and
Zhang Ju-Ren
Wang Xian-Yan
Affiliation:
School of Life Science, Shandong University, Jinan 250100, China
Shan Xiao-Yi
Affiliation:
School of Life Science, Shandong University, Jinan 250100, China
Yang Ai-Fang
Affiliation:
School of Life Science, Shandong University, Jinan 250100, China
Zhang Ju-Ren*
Affiliation:
School of Life Science, Shandong University, Jinan 250100, China
*
*Corresponding author: Email: jrzhang@sdu.edu.cn
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Abstract

The plant expression vector pCAMBIA1300-AtNHX1-als was constructed by inserting the herbicide resistance gene als of Arabidopsis thaliana into the plasmid pCAMBIA1300-AtNHX1, which contains the AtNHX1 gene encoding the Na+/H+ antiport from the vacuolar membrane of A. thaliana. Transgenic tobacco plants were obtained via Agrobacterium-mediated transformation. PCR and Southern blot assay indicated that genes als and AtNHX1 had been integrated into the genome of the transgenic plants. The herbicide resistance and salt tolerance of transgenic plants increased by about 1000-fold and by 1.5% NaCl concentration, respectively, compared with controls. Herbicide resistance of the T1 progeny was evaluated by spraying transgenic plants with different concentrations of Luhuanglong at the four-leaf stage. Controls gradually died under 100 mg/l Luhuanglong whereas 73% of the T1 plants still survived at 500 mg/l Luhuanglong. Thus the plant expression vector pCAMBIA1300-AtNHX1-als could be used to improve the herbicide resistance and salt tolerance of crops.

Keywords

alsAtNHX1salt toleranceherbicide resistancetobacco
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 1 , Issue 2 , August 2004 , pp. 85 - 91
Copyright
Copyright © China Agricultural University and Cambridge University Press 2004

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