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In vitro fertilization and cleavage of mouse oocytes recombined with the first polar body

Published online by Cambridge University Press:  01 October 2008

Wang Gong-Jin ,
Tan Xiao-Dong ,
Zhou Xiao-Long ,
Xu Xiao-Bo  and
Fan Bi-Qin
Wang Gong-Jin*
Affiliation:
Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
Tan Xiao-Dong
Affiliation:
Nanjing Normal University, Nanjing 210097, China
Zhou Xiao-Long
Affiliation:
Nanjing Normal University, Nanjing 210097, China
Xu Xiao-Bo
Affiliation:
Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
Fan Bi-Qin
Affiliation:
Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
*
*Corresponding author. E-mail: wgjphd@yahoo.cn
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Abstract

The developmental functions of oocytes of three strains of mice (Kunming, ICR and C57BL/6-Tg(CAG-EGFP)C14-Y01-FM131Osb) recombined with the nuclei of first polar bodies (Pbs I) were explored. Cumulus oocyte complexes (COCs) from the mice were collected after superovulation, then Pbs I were obtained from the COCs by 2% pronase treatment. The survival of Pbs I under different temperatures was identified by morphology and trypan blue staining. Later, the polar body I (Pb 1) nucleus and a little cytoplasm was injected into each oocyte, the nuclei of which had been enucleated by micromanipulation. Oocytes recombined with Pbs I were fertilized, then cultured in vitro in order to observe their further development. The results showed that the vigour of Pbs I was maintained for 12–14 h after superovulation, and was still maintained after 48 h at 4 °C. A total of 13 out of 117 recombined oocytes from Kunming and ICR mice, as well as 3 out of 38 recombined oocytes from C57BL/6-Tg(CAG-EGFP)C14-Y01-FM131Osb mice, developed into two-cell embryos. The experiments confirmed that mouse oocytes recombined with the nuclei of Pbs I could maintain fertilization and development. These results present valuable references for further utilization of genetic resources for farm animals

Keywords

mouse first polar bodyrecombination of oocytein vitro fertilizationdevelopment in vitro
Type
Research Papers
Information
Chinese Journal of Agricultural Biotechnology , Volume 5 , Issue 2 , August 2008 , pp. 169 - 173
Copyright
Copyright © China Agricultural University 2008

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Footnotes

First published in Journal of Agricultural Biotechnology 2008, 16(1): 77–81

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