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Effect of insulin on transcription of lipogenic and lipolytic-related genes and lipid metabolism in primary porcine adipocytes
Published online by Cambridge University Press: 12 February 2007
Abstract
Primary adipocytes from subcutaneous adipose tissue samples obtained from 7-day-old Yorkshire×Landrace crossbreed piglets were exposed to 0–400 nmol/l of insulin for 48 h. The accumulated triglyceride was measured through Oil Red O staining and the cumulative glycerol released was determined to assess lipolytic activity in adipocytes. Transcription levels of sterol regulatory element binding protein (SREBP)-1c, carbohydrate response element binding protein (ChREBP), acetyl-CoA carboxylase 1 (ACC1), fatty acid synthase (FAS), and hormone-sensitive lipase (HSL) were assessed using reverse transcriptase-polymerase chain reaction (RT-PCR). The results showed that ChREBP and ACC1 mRNA levels were not influenced by insulin alone under low glucose (5 nmol/l). FAS mRNA level was markedly stimulated by all doses of insulin except 200 nmol/l, and SREBP-1c mRNA level increased with 100–300 nmol/l insulin. High insulin doses (300 and 400 nmol/l) increased the HSL mRNA level as well as lipolytic activity.
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- Copyright © China Agricultural University and Cambridge University Press 2006
References
Azain, MJ (2004) Role of fatty acid in adipocyte growth and development. Journal of Animal Science 82: 916–924.CrossRefGoogle ScholarPubMed
Bécard, D, Hainault, I and Dalila, AM (2001) Adenovirus-mediated overexpression of sterol regulatory element binding protein-1c mimics insulin effects on hepatic gene expression and glucose homeostasis in diabetic mice. Diabetes 50: 2425–2430.CrossRefGoogle ScholarPubMed
Bertile, F, Criscuolo, F, Oudart, H, Maho, YL and Raclot, T (2003) Difference in the expression of lipolytic-related genes in rat white adipose tissues. Biochemical and Biophysical Research Communications 307: 540–546.Google Scholar
Ding, ST, Shinckel, AP, Weber, TE and Mersmann, HJ (2000) Expression of porcine transcription factors and genes related to fatty acid metabolism in different tissue and genetic populations. Journal of Animal Science 78: 2127–2134.CrossRefGoogle ScholarPubMed
Eberlé, D, Hegarty, B, Bossard, P, Ferré, P and Foufelle, F (2004) SREBP transcription factors: Master regulators of lipid homeostasis. Biochimie 86: 839–848.CrossRefGoogle ScholarPubMed
Edwards, PA, Tabor, DT and Kast, HR (2000) Regulation of gene expression by SREBP and SCAP. Biochimica et Biophysica Acta (BBA)–Molecular and Cell Biology of Lipids 1529: 103–113.Google Scholar
Green, A, Rumberger, JM, Stuart, CA and Ruhoff, MS (2004) Stimulation of lipolysis by tumor necrosis factor-α in 3T3-L1 adipocytes is glucose dependent: Implications for long-term regulation of lipolysis. Diabetes 53: 74–81.Google Scholar
Ishii, S, IIzuka, K, Miller, BC and Uyeda, K (2004) Carbohydrate response element binding protein directly promotes lipogenic enzyme gene transcription. Proceedings of the National Academy Sciences of the USA 101: 15597–15602.Google Scholar
Koo, SH, Angela, KD and Towle, HC (2001) Glucose and insulin function through two distinct transcription factors to stimulate expression of lipogenic enzyme genes in liver. Journal of Biological Chemistry 276: 9437–9445.CrossRefGoogle ScholarPubMed
Lee, KC, Azain, MJ, Hausman, DB and Ramsay, TG (2000) Somatotropin and adipose tissue metabolism: Substrate and temporal effects. Journal of Animal Science 78: 1236–1246.CrossRefGoogle ScholarPubMed
Lin, YQ, Li, RW, Sun, C and Yang, GS (2004) Rat adipocyte apoptosis induced by tumor necrosis factor-α. Journal of Agriculture Biotechnology 12(6): 644–647 (in Chinese with English abstract).Google Scholar
Louveau, I and Gondret, F (2004) GH and insulin affect fatty acid synthase activity in isolated porcine adipocytes in culture without any modifications of sterol regulatory element binding protein-1 expression. Journal of Endocrinology 181: 271–280.CrossRefGoogle ScholarPubMed
Mao, JQ, Chirala, SS and Wakil, SJ (2003) Human acetyl-CoA carboxylase 1 gene: Presence of three promoters and heterogeneity at the 5'-untranslated mRNA region. Proceedings of the National Academy Sciences of the USA 100: 7515–7520.CrossRefGoogle ScholarPubMed
Marone, M, Mozzetti, S, Ratis, DD, Pierelli, L and Scambia, G (2001) Semiquantitative RT-PCR analysis to assess the expression levels of multiple transcripts from the same sample. Biological Procedures Online 3(1): 19–25.CrossRefGoogle ScholarPubMed
McTernan, PG, Harte, AL, Anderson, LA, Green, A, Eggo, MC and Kumar, S (2002) Insulin and rosiglitazone regulation of lipolysis and lipogenesis in human adipose tissue in vitro. Diabetes 51: 1493–1498.CrossRefGoogle ScholarPubMed
Palmer, DG, Rutter, GA and Jeremy, MT (2002) Insulin-stimulated fatty acid synthase gene expression does not require increased sterol response element binding protein 1 transcription in primary adipocytes. Biochemical and Biophysical Research Communications 291: 439–443.CrossRefGoogle Scholar
Ramirez, ZJL, Munozledo, FC and Harcuch, W (1992) Quantitation of adipose conversion and triglycerides by staining intracytoplasmic lipids with Oil Red O. Histochemistry 97: 493–497.Google Scholar
Semenkovich, CF (1997) Regulation of fatty acid synthase. Progress in Lipid Research 36: 43–53.CrossRefGoogle ScholarPubMed
Smih, F, Rouet, P, Lucas, S, Mairal, A, Sengenes, C and Langin, D (2002) Transcriptional regulation of adipocyte hormone-sensitive lipase by glucose. Diabetes 51: 293–300.CrossRefGoogle ScholarPubMed
Vaulont, S, Vasseur, CM and Kahn, A (2000) Glucose regulation of gene transcription. Journal of Biological Chemistry 275: 31555–31558.Google Scholar