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HSP70 and HSP70 mRNA localization in heat-stressed tissues of broilers

Published online by Cambridge University Press:  13 February 2008

Sun Pei-Ming ,
Liu Yu-Tian ,
Wang Qing-Hua ,
Wang Zhi-Liang  and
Bao En-Dong
Sun Pei-Ming
Affiliation:
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China College of Agroforestry Sciences, Linyi University, Linyi 276005, China
Liu Yu-Tian
Affiliation:
National Diagnostic Centre for Exotic Animal Disease, National Animal Quarantine Institute of Ministry of Agriculture, Qingdao 266032, China
Wang Qing-Hua
Affiliation:
National Diagnostic Centre for Exotic Animal Disease, National Animal Quarantine Institute of Ministry of Agriculture, Qingdao 266032, China
Wang Zhi-Liang
Affiliation:
National Diagnostic Centre for Exotic Animal Disease, National Animal Quarantine Institute of Ministry of Agriculture, Qingdao 266032, China
Bao En-Dong*
Affiliation:
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China
*
*Corresponding author. E-mail: b-endong@njau.edu.cn
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Abstract

The localization of heat shock protein 70 (HSP70) and HSP70 mRNA in the heart, liver, lung, kidney, spleen, thymus and cloacal bursa in broilers that were heat stressed for 6 h was conducted using immunohistochemistry and in situ hybridization techniques. Positive HSP70 mRNA signals were detected in the liver and lung, especially in the vessel walls. A weak presence was found in the myocardial cells. No significant signals were observed in spleen, thymus and cloacal bursa. HSP70 was observed in the vessel walls of all investigated broiler tissues. Localizations of HSP70 and HSP70 mRNA suggest that HSP70 could be correlated with cardiovascular function.

Keywords

heat shock protein 70cardiovascular systemin situ hybridizationimmunohistochemistrybroiler
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 4 , Issue 3 , December 2007 , pp. 181 - 186
Copyright
Copyright © China Agricultural University and Cambridge University Press 2007

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Footnotes

First published in Journal of Agricultural Biotechnology 2007, 15(3): 404–408

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