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Effect of dietary supplements commonly used in Standardbred racing on plasma total carbon dioxide

Published online by Cambridge University Press:  15 April 2010

Valerie Balaskonis
Affiliation:
Department of Animal Science, Equine Science Center, Rutgers, The State University of New Jersey, New Brunswick, NJ, USA
Amanda Szucsik
Affiliation:
Department of Animal Science, Equine Science Center, Rutgers, The State University of New Jersey, New Brunswick, NJ, USA
Robert A. Lehnhard
Affiliation:
Department of Kinesiology, University of Maine, Orono, ME, USA
Kenneth H. McKeever*
Affiliation:
Department of Animal Science, Equine Science Center, Rutgers, The State University of New Jersey, New Brunswick, NJ, USA
*
*Corresponding author: mckeever@aesop.rutgers.edu
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Abstract

Ten mature Standardbred mares (9–13 years, ~522 kg) were used to test the hypothesis that pelleted dietary supplementation would alter total plasma bicarbonate (tCO2) concentrations. All the mares used in this study were unconditioned, but were familiarized to the laboratory setting and running on a treadmill. Each of the ten mares was semi-randomly assigned one of four dietary treatments. The four treatments (oats as control and three pelleted feed supplements: Drive, Omolene and Strategy) were administered in a crossover fashion throughout a 4-week testing period. These products were chosen based on the frequency of their use by Standardbred/Thoroughbred owners and trainers in New Jersey. The horses underwent a simulated race test (SRT) on a treadmill (6% grade) at the end of each administration period. During the SRT, horses ran for 2 min at 4 m s− 1, 2 min at the speed previously shown to correspond to VO2max and 2 min at 4 m s− 1. Blood was collected before supplement treatment ( − 4 h), 10 min prior to exercise and at 0, 60 and 90 min post-exercise. Plasma concentrations of bicarbonate, sodium, potassium and chloride were measured using a Beckman ELISE analyser. The major finding of this study indicates that the plasma [tCO2], chloride and sodium concentrations were not altered by the dietary supplements studied (P>0.05). There were differences (P < 0.05) in plasma [tCO2] across sampling intervals ( − 4 h, − 10 min, +0 min, +60 min and +90 min) that were attributable to acute exercise (mean ± SE: 34.4 ± 0.9, 33.2 ± 1.1, 20.2 ± 0.8, 31.5 ± 0.8, 30.3 ± 1.6 mmol l− 1). There was a slight effect of treatment (P < − 0.05) on potassium levels. However, exercise was the main factor that caused substantial changes (P < 0.05) in the plasma tCO2, potassium, haematocrit and total protein concentrations. It was concluded that the pelleted diet supplements examined do not alter plasma [tCO2] in horses.

Type
Research Paper
Copyright
Copyright © Cambridge University Press 2010

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References

1Frey, LP, Kline, KH, Foreman, JH, Brady, AH and Cooper, SR (1995). Effects of warming up, racing and sodium bicarbonate in Standardbred horses. Equine Veterinary Journal Supplement 18: 310313.CrossRefGoogle Scholar
2Roelofson, R (1992). Sucrose and bicarbonate overloading in Standardbred horses in Ontario. Proceedings of 9th International Conference of Racing Analysts and Veterinarians. New Orleans, LA: Louisiana State University, pp. 145217.Google Scholar
3Rose, RJ and Lloyd, DR (1992). Sodium bicarbonate: more than just a milkshake? Equine Veterinary Journal 24: 7576.CrossRefGoogle ScholarPubMed
4Lloyd, DR, Reilly, P and Rose, RJ (1992). The detection and performance effects of sodium bicarbonate administration in the racehorse. Proceedings of the 9th International Conference of Racing Analysts and Veterinarians. New Orleans, LA: Louisiana State University, pp. 131138.Google Scholar
5Lloyd, DR and Rose, RJ (1995). Effects of sodium bicarbonate on acid–base status and exercise capacity. Equine Veterinary Journal Supplement 18: 323325.CrossRefGoogle Scholar
6Lloyd, DR and Rose, RJ (1992). Issues relating to the use of products that can produce metabolic alkalosis prior to racing. Australian Equine Veterinary Journal 10: 2728.Google Scholar
7Frey, LP, Kline, KH, Foreman, JH, Lyman, JT and Butadom, P (1999). Effects of alternate alkalinizing compounds on blood plasma acid–base balance in exercising horses. Proceedings of the 16th Equine Nutrition and Physiology Society Symposium. Raleigh, NC: Equine Nutrition and Physiology Society, pp. 161162.Google Scholar
8Slocumbe, R, Huntington, P, Lind, KL and Vine, JL (1995). Plasma total CO2 and electrolytes: diurnal changes and effects of adrenaline, doxapram, rebreathing, and transport. Equine Veterinary Journal Supplement 18: 331336.CrossRefGoogle Scholar
9Auer, DE, Skelton, S, Tay, S and Badlock, FC (1993). Detection of bicarbonate administration (milkshake) in Standardbred horses. Austrian Veterinary Journal 70: 336340.CrossRefGoogle ScholarPubMed
10Irvine, CHG (1992). Control of administration of sodium bicarbonate and other alkalis: the New Zealand experience. Proceedings of the 9th International Conference of Racing and Analysts and Veterinarians. New Orleans, LA: Louisiana State University, pp. 139143.Google Scholar
11Lorimer, P (1998). Report of the NJ State Police Racing Commission Drug Detection Laboratory.Google Scholar
12Dyer, K (2000). TCO2 questions and answers. Annual General Meeting. http://www.harness.org.au/ausbreed/papers/TCO2RPT.HTM.Google Scholar
13Kauffman, K, Kline, KH, Forman, JH and Lyman, JT (1999). Effects of diet on plasma TCO2 in horses. Proceedings of the 16th Equine Nutrition and Physiology Society Symposium. Raleigh, NC: Equine Nutrition and Physiology Society, pp. 363364.Google Scholar
14Mueller, RK, Topliff, DR, Freeman, DW, MacAllister, C, Carter, SD and Cooper, SR (1999). Effect of varying DCAD on the acid–base status of mature sedentary horses with varying starch source and level of intake. Animal Science Research Report. Stillwater, OK: Oklahoma State University.Google Scholar
15Ciolino, D, Lehnhard, RA and McKeever, KH (2009). Effect of Gastrogard on plasma tCO2 concentration in Standardbred horses. Comparative Exercise Physiology 6: 8187.CrossRefGoogle Scholar
16Caltibilota, TJ, Milizio, JG, Malone, SR, Kenney, J and McKeever, KH (2010). Effect of Sucralfate® on plasma total carbon dioxide concentration in horses subjected to a simulated race test. The Veterinary Journal 183: 191195.CrossRefGoogle Scholar
17Szucsik, A, Balaskonis, V and McKeever, KH (2006). Effect of seven common supplements on plasma electrolyte and total carbon dioxide concentration and strong ion difference in Standardbred horses subjected to a simulated race test. Equine and Comparative Exercise Physiology 3: 3744.CrossRefGoogle Scholar
18Stewart, PA (1978). Independent and dependent variables of acid–base control. Respiration Physiology 33: 926.CrossRefGoogle ScholarPubMed
19Constable, PD (1997). A simplified strong ion model for acid–base equilibria: application to horse plasma. Journal of Applied Physiology 83: 297311.CrossRefGoogle ScholarPubMed
20Ralston, SL, Puzio, C and Cuddeford, D (1993). Dietary carbohydrates, acid base status and urinary calcium and phosphorus excretion in horses. Proceedings of 13th Equine Nutrition and Physiology Society Symposium. Gainesville, FL: Equine Nutrition and Physiology Society, pp. 4243.Google Scholar
21Carlson, GP (1987). Hematology and body fluids in the equine: a review. In: Equine Exercise Physiology 2. Davis CA: ICEEP Publications, pp. 393425.Google Scholar
22McKeever, KH, Hinchcliff, KW, Reed, SM and Robertson, JT (1993). Role of decreased plasma volume in hematocrit alterations during incremental treadmill exercise in horses. American Journal of Physiology – Regulatory, Integrative and Comparative Physiology 265: R404R408.CrossRefGoogle ScholarPubMed