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XXXI.—Scottish National Antarctic Expedition: A Contribution to the Histology of the Central Nervous System of the Weddell Seal (Leptonychotes weddellii).

Published online by Cambridge University Press:  06 July 2012

Harold Axel Haig
Harold Axel Haig
Affiliation:
Lecturer in Histology and Embryology, University College, Cardiff.
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Extract

The specimens submitted for examination were:

(a) Portions of the brain (labelled Specimen XXXI.).

(b) Portions of spinal cord (labelled Specimen XXIV.).

Both were in excellent condition as regards fixation and hardening, having been preserved for many years in a fluid composed of formol and 95 per cent. alcohol (the fluid was also injected into the cerebral vessels). They were, previous to histological examination, submitted to the following processes:—

i. Comparatively thin slices were taken from various regions and placed for twenty-four hours in absolute alcohol.

ii. Then transferred to acetone for twelve hours.

iii. Placed in xylol until permeated.

iv. Embedded in paraffin of melting-point 52° C. Sections were then taken with an improved form of the Cambridge rocking microtome, and fixed to slides by means of the albumen method.

Type
Research Article
Information
Earth and Environmental Science Transactions of The Royal Society of Edinburgh , Volume 48 , Issue 4 , 1913 , pp. 849 - 866
Copyright
Copyright © Royal Society of Edinburgh 1913

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References

page 850 note * The pituitary gland sections were stained with Delafield's hæmatoxylin, followed by eosin to differentiate.

page 855 note * The decussation of the pyramids is of greater extent longitudinally than in the human medulla, being found quite close to the calamus scriptorius at its upper level.

page 862 note * It is possible that this structure is represented in some of the lower types by the sacci vasculosi found in connection with the pituitary gland.

page 864 note * In these clumps, some of the cells possess much clearer cytoplasm, which is not deeply stained with eosin; in this respect the anterior lobe resembles that of the human pituitary gland.