Hostname: page-component-7479d7b7d-t6hkb Total loading time: 0 Render date: 2024-07-09T19:51:47.684Z Has data issue: false hasContentIssue false
  • English
  • Français

Detection of feral transgenic oilseed rapewith multiple-herbicide resistance in Japan

Published online by Cambridge University Press:  08 December 2006

Mitsuko Aono ,
Seiji Wakiyama ,
Masato Nagatsu ,
Nobuyoshi Nakajima ,
Masanori Tamaoki ,
Akihiro Kubo  and
Hikaru Saji
Mitsuko Aono
Affiliation:
Environmental Biology Division, National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, 305-8506, Japan
Seiji Wakiyama
Affiliation:
Japan Wildlife Research Center, 3-10-10 Shitaya, Taito-Ku, Tokyo, 110-8676, Japan
Masato Nagatsu
Affiliation:
Japan Wildlife Research Center, 3-10-10 Shitaya, Taito-Ku, Tokyo, 110-8676, Japan
Nobuyoshi Nakajima
Affiliation:
Environmental Biology Division, National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, 305-8506, Japan
Masanori Tamaoki
Affiliation:
Environmental Biology Division, National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, 305-8506, Japan
Akihiro Kubo
Affiliation:
Environmental Biology Division, National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, 305-8506, Japan
Hikaru Saji
Affiliation:
Environmental Biology Division, National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, 305-8506, Japan

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Repeated monitoring for escaped transgenic crop plants is sometimes necessary, especially in cases when the crop has not been approved for release into the environment. Transgenic oilseed rape (Brassica napus) was detected along roadsides in central Japan in a previous study. The goal of the current study was to monitor the distribution of transgenic oilseed rape and occurrence of hybridization of transgenic B. napus with feral populations of its closely related species (B. rapa and B. juncea) in the west of Japan in 2005. The progenies of 50 B. napus, 82 B. rapa and 283 B. juncea maternal plants from 95 sampling sites in seven port areas were screened for herbicide-resistance. Transgenic herbicide-resistant seeds were detected from 12 B. napus maternal plants growing at seven sampling sites in two port areas. A portion of the progeny from two transgenic B. napus plants had both glyphosate-resistance and glufosinate-resistance transgenes. Therefore, two types of transgenic B. napus plants are likely to have outcrossed with each other, since the double-herbicide-resistant transgenic strain of oilseed rape has not been developed intentionally for commercial purposes. As found in the previous study, no transgenic seeds were detected from B. rapa or B. juncea, and more extensive sampling is needed to determine whether introgression into these wild species has occurred.

Keywords

Brassicaestablishmentglufosinateglyphosateherbicideintrogressionoutcrossingtransgenic plant
Type
Research Article
Information
Environmental Biosafety Research , Volume 5 , Issue 2 , April 2006 , pp. 77 - 87
Copyright
© ISBR, EDP Sciences, 2006

References

Barry GF, Kishore GM (October 31, 1995) Glyphosate tolerant plants. United States, Patent No. 5463175
Barry GF, Kishore GM, Padgette SR, Taylor ML, Kolacz KH, Weldon M, Re DB, Eichholtz DA, Fincher K, Hallas L (1992) Inhibitors of amino acid biosynthesis: Strategies for imparting glyphosate tolerance to crop plants. In Singh BK, Flores HE, Shannon JC, eds, Biosynthesis and Molecular Regulation of Amino Acids in Plants, American Society Plant Physiologists, Rockville, MD, USA, pp 139–145
Becker, HC, Damgaard, C, Karlsson, B (1992) Environmental variation for outcrossing rate in rapeseed (Brassica napus). Theor. Appl. Genet. 84: 303–306
Beckie, HJ, Warwick, SI, Nair, H, Séguin-Swartz G (2003) Gene flow in commercial fields of herbicide-resistant canola (Brassica napus). Ecol. Appl. 13: 1276–1294 CrossRef
Bing, DJ, Downey, RK, Rakow, GFW (1996) Hybridizations among Brassica napus, B. rapa and B. juncea and their two weedy relatives B. nigra and Sinapis arvensis under open pollination conditions in the field. Plant Breed. 115: 470–473 CrossRef
Comai, L, Facciotti, D, Hiatt, WR, Thompson, G, Rose, RE, Stalker, DM (1985) Expression in plants of a mutant aroA gene from Salmonella typhimurium confers tolerance to glyphosate. Nature 317: 741–744 CrossRef
Crawley, MJ, Brown, SL, Hails, RS, Kohn, DD, Rees, M (2001) Transgenic crops in natural habitats. Nature 409: 682–683 CrossRef
EFSA (the European Food Safety Authority) (2004) Guidance document of the scientific panel on genetically modified organisms for the risk assessment of genetically modified plants and derived food and feed. http://www.efsa.eu.int/science/gmo/gmo_guidance/660_en.html
Friesen, LF, Nelson, AG, Van Acker, RC (2003) Evidence of contamination of pedigreed canola (Brassica napus) seedlots in western Canada with genetically engineered herbicide resistance traits. Agron. J. 95: 1342–1347 CrossRef
Hall, L, Topinka, K, Huffman, J, Davis, L, Good, A (2000) Pollen flow between herbicide-resistant Brassica napus is the cause of multiple-resistant B. napus volunteers. Weed Sci. 48: 688–694 CrossRef
ISAAA (International Service for the Acquisition of Agri-biotech Applications) (2006) ISAAA Briefs 34-2005: Global Status of Commercialized Biotech/GM Crops: 2005. http://www.isaaa.org/
Jørgensen, RB, Andersen, B, Landbo, L, Mikkelsen, TR (1996) Spontaneous hybridization between oilseed rape (Brassica napus) and weedy relatives. Acta Horticul. 407: 193–197 CrossRef
Mariani, C, De Beuckeleer, M, Truettner, J, Leemans, J, Goldberg, RB (1990) Induction of male sterility in plants by a chimaeric ribonuclease gene. Nature 347: 737–741 CrossRef
Mariani, C, Gossele, V, De Beuckeleer, M, De Block, M, Goldberg, RB, De Greef, W, Leemans, J (1992) A chimaeric ribonuclease-inhibitor gene restores fertility to male sterile plants. Nature 357: 384–387 CrossRef
OECD (Organisation for Economic Cooperation and Development) (1997) Series on harmonization of regulatory oversight in biotechnology No.7; Consensus document on the biology of Brassica napus L. (oilseed rape). http://www.olis.oecd.org/olis/1997doc.nsf/LinkTo/ocde-gd(97)63
OGTR (Office of the Gene Technology Regulator) (2002) The biology and ecology of canola (Brassica napus). http://www.ogtr.gov.au/pdf/ir/brassica.pdf
Padgette SR, Kolacz KH, Delannay X, Re DB, Lavallee BJ, Tinius CN, Rhodes WK, Otero YI, Barry GF, Eichholtz DA, Peschke VM, Nida DL, Taylor NB, Kishore GM (1995) Development, identification, and characterization of a glyphosate-tolerant soybean line. Crop Sci., 35: 1451–1461
Saji, H, Nakajima, N, Aono, M, Tamaoki, M, Kubo, A, Wakiyama, S, Hatase, Y, Nagatsu, M (2005) Monitoring the escape of transgenic oilseed rape around Japanese ports and roadsides. Environ. Biosafety Res. 4: 217–222 CrossRef
Simard, MJ, Légère, A, Séguin-Swartz, G, Nair, H, Warwick, S (2005) Fitness of double vs. single herbicide-resistant canola source. Weed Sci. 53: 489–498 CrossRef
Stalker, DM, McBride, KE, Malyj, LD (1988) Herbicide resistance in transgenic plants expressing a bacterial detoxification gene. Science 242: 419-423 CrossRef
Thompson CJ, Movva NR, Tizard R, Crameri R, Davies JE, Lauwereys M, Botterman J (1987) Characterization of the herbicide-resistance gene bar from Streptomyces hygroscopicus. EMBO J. 6: 2519–2523
USDA (United States Department of Agriculture) (2005a) USDA Foreign Agricultural Service, Global Agriculture Information Network Report, Australia Biotechnology Agricultural Biotechnology Annual Report 2005, GAIN Report Number AS5024. http://www.fas.usda.gov/gainfiles/200507/146130258.doc
USDA (United States Department of Agriculture) (2005b) USDA Foreign Agricultural Service, Global Agriculture Information Network Report, Canada Biotechnology Agricultural Biotechnology Report 2005, GAIN Report Number CA5044. http://www.fas.usda.gov/gainfiles/200507/146130196.doc
Warwick S, Beckie HJ, Simard MJ, Légère A, Nair H, Séguin-Swartz G (2004) Environmental and agronomic consequences of herbicide-resistant (HR) canola in Canada. In Den Nijs HCM, Bartsch D, Sweet J, eds, Introgression from Genetically Modified Plants into Wild Relatives, CABI publishing, Wallingford, Oxfordshire, UK, pp 323–337
Williams, IH, Martin, AP, White, RP (1986) The pollination requirements of oil-seed rape (Brassica napus L.). J. Agric. Sci. 106: 27–30 CrossRef