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Airborne micro-organisms: a technique for studying their survival

Published online by Cambridge University Press:  15 May 2009

G. J. Harper ,
A. M. Hood  and
J. D. Morton
G. J. Harper
Affiliation:
Microbiological Research Establishment, Porton, near Salisbury, Wilts
A. M. Hood
Affiliation:
Microbiological Research Establishment, Porton, near Salisbury, Wilts
J. D. Morton
Affiliation:
Microbiological Research Establishment, Porton, near Salisbury, Wilts
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The concentration of viable cells in an aerosol of micro-organisms is reduced by physical loss and biological decay. To study biological decay it is necessary to distinguish between the two processes. The preferred way of doing this is to mix the test aerosol with a tracer which is subject only to physical loss: the tracer and test cells should be mixed before dissemination.

The recommended tracers for bacterial clouds are bacteria made radioactive by growing on a medium containing 32P, then killed with formaldehyde and washed. To ensure closest identity of physical behaviour it is necessary that the tracer should be of the same species as the cells under test.

We are grateful to Mr E. O. Powell for helpful comment during the preparation of this paper.

Type
Research Article
Information
Journal of Hygiene , Volume 56 , Issue 3 , September 1958 , pp. 364 - 370
Copyright
Copyright © Cambridge University Press 1958

References

Harper, G. J. & Morton, J. D. (1952). J. gen. Microbiol. 7, 98.CrossRefGoogle Scholar
Henderson, D. W. (1952). J. Hyg., Camb., 50, 53.CrossRefGoogle Scholar
May, K. R. & Harper, G. J. (1957). Brit. J. industr. Med. 14, 287.Google Scholar