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An analysis of the diversity of Haemophilus parainfluenzae in the adult human respiratory tract by genomic DNA fingerprinting

Published online by Cambridge University Press:  15 May 2009

G. R. D. Kerr ,
K. J. Forbes ,
A. Williams  and
T. H. Pennington
G. R. D. Kerr
Affiliation:
British Antarctic Survey Medical Unit, Robert Gordons Institute of Technology Survival Centre, King Street, Aberdeen AB2 3BJ
K. J. Forbes*
Affiliation:
Department of Medical Microbiology, University of Aberdeen, Foresterhill, Aberdeen AB9 2ZD
A. Williams
Affiliation:
Department of Medical Microbiology, University of Aberdeen, Foresterhill, Aberdeen AB9 2ZD
T. H. Pennington
Affiliation:
Department of Medical Microbiology, University of Aberdeen, Foresterhill, Aberdeen AB9 2ZD
*
* Author for correspondence.
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Summary

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A method for typing Haemophilus species is described, based on the analysis of genomic DNA from Haemophilus parainfluenzae. The DNA was extracted by a rapid method and digested with the restriction enzyme BamHI to provide a characteristic ‘fingerprint’. The pattern of fragments in the ranges 1–1·6 kb, 1·6–2 kb and 2–3 kb were used to produce a numerical profile of each isolate. In total 97 isolates were examined; 88 from throat swab material isolated from the 15 members of a British Antarctic Survey base and 9 type strains. Seventy-two of the 88 antarctic isolates were H. parainfluenzae and were found to be very diverse, comprising 41 identifiable strains with up to 5 strains being isolated from a single throat swab sample. There was evidence for both carriage and transmission within the isolated community. The technique provided a highly discriminatory method for characterizing Haemophilus strains which is suitable for epidemiological studies.

Type
Research Article
Information
Epidemiology & Infection , Volume 111 , Issue 1 , August 1993 , pp. 89 - 98
Copyright
Copyright © Cambridge University Press 1993

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