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An Experimental Study of the Neisser-Wechsberg Phenomenon

Published online by Cambridge University Press:  15 May 2009

C. G. Pandit
C. G. Pandit
Affiliation:
From the Bacteriological Laboratory, University of London, King's College.
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(1) The inhibiting phenomenon of Neisser and Wechsberg, as frequently observed with bacteriolytic sera, can also be shown to occur with haemolytic sera.

(2) This phenomenon develops early during immunisation, when it is very marked; but as immunisation proceeds it tends to disappear.

(3) In sera examined some time after their removal the inhibiting faculty is seen to disappear first, before any marked change is noticed in its bactericidal titre. This can be shown to occur in vivo also.

(4) The inhibiting faculty is observed only with inactivated sera; fresh and active sera showing complete bactericidal activity at all times. The effects are not however due to complementoids produced by inactivation.

(5) The phenomenon is not due to the action of any of the known anti bodies, or special “inhibiting antibodies”; but it is due probably to dis sociation of the antigen-amboceptor complex. Such dissociation only occurs when amboceptors are present in excess. This explains why inhibition occurs only with high doses of an immune serum.

The serum to be used should be without an antiseptic. Commercially prepared antisera usually contain a small amount of it. It is shown that corpuscles sensitised in them undergo haemolysis without the addition of complement (see p. 427).

(6) Since it is known that the union of antigen and amboceptor is not always a firm one, it is suggested that during early days of immunisation amboceptors of a weak combining affinity are produced. These are replaced later by those which are capable of a more permanent union with them (antigens).

(7) The name “complement-blocking” is not an appropriate one, as com plement could be shown to exist free in the inhibiting fluids. It should rather be spoken of as an “amboceptor-dissociation” effect.

(8) Inhibition is specific. An attempt was made to utilise this fact in differ entiation of strains of true cholera but it was found to have no definite diagnostic value.

The writer wishes to express his indebtedness to Prof. Hewlett for sug gesting the problem to him and also for his kind guidance and encouragement during the course of the experiments. His thanks are also due to Dr Taylor for his valuable assistance.

Type
Research Article
Information
Journal of Hygiene , Volume 21 , Issue 4 , August 1923 , pp. 406 - 439
Copyright
Copyright © Cambridge University Press 1923

References

REFERENCES

Bordet, and Gay, (1906). [On the ralations of sensitisers to alexine.] Ann. Inst. Pasteur, XX. 467. Also Bordet and Gay (1909), Studies in Immunity, p. 363. Whiley and Sons, New York.Google Scholar
Brekke, (1916). Quoted by Thjøotta.Google Scholar
Coplans, (1922). Bactericidal action upon calf lymph...etc. Journ. Path. and Bact. XXX. 181–2.Google Scholar
Douglas, (1921). Question of Serological races of Cholera. Brit. Journ. Of Experimental Path. II. 49.Google Scholar
Ehrlich, (1902). Berl. Klin. Wochenschr. XXI.Google Scholar
Ehrlich, and Sachs, (1902). [Concerning the Mechanism of the action of Amboceptors.] Berl. klin. Wochenschr. XXI. Also Ehrlich and Bolduan (1910), Studies in Immunity, p. 209. Wiley and Sons.Google Scholar
Jervel, F. (1921). Influence of temperature on agglutination. Journ. Immunology, Nov. 1921.Google Scholar
Gay, (1905,1). Fixation of alexine by serum precipitate, p. 346.Google Scholar
Gay, (1905,2). Deviation of the alexine in Haemolysis, p. 357. Also Studies in Immunity (Bordet and Gay).Google Scholar
Georgi, W. (1920). Ueber die hãmolytische Wirkung des Meerschweinchen-Serums im Salzarmenmedium. Ztschr.f.Immunitãtsforsch. u. exper. Therap. I Orig. 29.Google Scholar
Grüber, (1901). Quoted by Thiøtta and Lipstein.Google Scholar
Huntoon, (3.1921). Antibody Studies. Journ. Immunology, p. 121.Google Scholar
Leclainche, and Morel, (1901). La S´eroth´rapie de la septic´mie gangréneuse. Ann. Inst.Pasteur, XV.Google Scholar
Lipstein, (1902). Die Komplementablenkung bei baktericiden Reagenzglasversuchen undiher Ursache. Centralb. f. Bakt. Orig. XIX. 460.Google Scholar
Löffler, and Able, (1986). Ueber die spezifischen Eigenschaften der Schutzkörper im Blute Typhus-und Coli-immuner Tiere. Centralb.f.Bakt. XIX. 60.Google Scholar
Metchnikoff, (1905). Immunity in infectious diseases, p. 313.Google Scholar
Morgenroth, (1903). The binding of haemolytic amboceptors. Studies in immunity, p. 595(Ehrlich-Bolduan).Google Scholar
Muir, and Browning, (1905). On Chemical Combination and Toxic Action as exemplified in Haemolytic Sera. Proc. Roy. Society, LXXIV. 298.Google Scholar
Muir, and Browning, (1906). On the properties of anti-immure bodies and complementoids. Journ. Hygiene, VI.Google Scholar
Muir, and Martin, (1906). On the deviation of complement by a serum and its antiserum and its relation to the Precipitin test, Journ. Hygiene, VI. 282.Google Scholar
Neisser, and Wecjsberg, (1901). Ueber die Wirkungsart bactericider Sera. Münch. med. Wochenschr. V. 48, 679. Also Ehrlich, Studies in Immunity, p. 121.Google Scholar
Otto, and Sachs, (1906). Dissociation Phenomena in the toxin-antitoxin combination, in Studies in Immunity (Ehrlich-Bolduan), p. 666.Google Scholar
Pfeiffer, R. (1895). Ueber die specifischen Anti-körper der Cholera, XX, 215.Google Scholar
Sormani, (1916). [A new explanation of the Neisser-Wechsberg Phenomenon.] Ztschr. f. Immunitãtsforsch. XXIV. 336, Orig.Google Scholar
Thjøtta, (1. 1920). On the so-called Neisser and Wechsberg phenomenon. Journ. Immunology, V.Google Scholar
Zinsser, (1918). Infection and Resistance, p. 263. Macmillan and Co.Google Scholar