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Bacteriophage inhibition by extracts from phage-insensitive bacteria of the genus Pseudomonas

Published online by Cambridge University Press:  15 May 2009

T. H. Mead  and
M. van den Ende
T. H. Mead
Affiliation:
The C.S.I.R. and University of Cape Town Virus Research Unit, Department of Pathology, University of Cape Town
M. van den Ende
Affiliation:
The C.S.I.R. and University of Cape Town Virus Research Unit, Department of Pathology, University of Cape Town
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1. An electrophoretically homogeneous antigen preparation extracted from a strain (LII) of Pseudomonas aeruginosa by trichloracetic acid inhibits four phages active against this strain, and two phages (5 and 14) to which it is resistant but which attack a mutant strain LIII–3 bi.

2. An extract prepared in the same way from strain LIII–3 bi has no antigenic or inhibitory properties. Lysis of LIII–3 bi organisms by chloroform, however, yields a potent inhibitor active against several phages.

3. TCA extracts from immunologically distinct Ps. strains AII, BII and GII also inhibit phages 5 and 14 to which these strains are resistant.

4. The effect of a third phage (13) on Ps. LIII–3 bi is activated by the extract from strain LII which normally inhibits phages 5 and 14. Prolonged contact with very dilute solutions of the extract also causes activation of phage 5. Activation of phage 14 has not been observed.

5. The above findings are discussed in relation to current theories of phage action and phage inhibition.

Type
Research Article
Information
Journal of Hygiene , Volume 51 , Issue 1 , March 1953 , pp. 108 - 124
Copyright
Copyright © Cambridge University Press 1953

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