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Biochemical and molecular characterization of Salmonella enterica serovar berta, and comparison of methods for typing

Published online by Cambridge University Press:  15 May 2009

J. E. Olsen ,
D. J. Brown ,
D. L. Baggesen  and
M. Bisgaard
J. E. Olsen*
Affiliation:
Department of Veterinary Microbiology, The Royal Veterinary and Agricultural University, Frederiksberg C., Denmark
D. J. Brown
Affiliation:
Department of Veterinary Microbiology, The Royal Veterinary and Agricultural University, Frederiksberg C., Denmark
D. L. Baggesen
Affiliation:
Department of Veterinary Microbiology, The Royal Veterinary and Agricultural University, Frederiksberg C., Denmark
M. Bisgaard
Affiliation:
Department of Veterinary Microbiology, The Royal Veterinary and Agricultural University, Frederiksberg C., Denmark Department of Avian Diseases, National Veterinary Laboratory, Frederiksberg C., Denmark
*
*J. E. Olsen, Department of Veterinary Microbiology. The Royal Veterinary and Agricultural University, 13 Bülowsvej, DK 1870 Frederiksberg C., Denmark.
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Summary

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Strains of Salmonella enterica serovar berta (S. berta) from Denmark and seven other countries have been characterized with the aim of developing a rational typing strategy in connection with outbreak investigations.

Biotyping divided the strains into H2S-positive (90%) and H2S-negative (10%) biovars. Six percent of the strains were resistant to one or more antimicrobial agents. Eighty-eight percent of the strains carried plasmids and 52 different plasmid profiles were recognized. Six of the common plasmid sizes in these profiles were shown by restriction enzyme analyses to contain more than one plasmid species. More than 90% of the strains had the same ribotype with the restriction enzymes Sma I and EcoR I and the same whole cell protein profile. Outer membrane protein profiles and isoenzyme profiles were identical in all S. berta analysed.

Plasmid profiling in combination with restriction enzyme analysis of plasmids seemed to be the most rational typing strategy for S. berta. The results indicated that S. berta strains regardless of geographical source or host are possibly clonal in nature.

Type
Research Article
Information
Epidemiology & Infection , Volume 108 , Issue 2 , April 1992 , pp. 243 - 260
Copyright
Copyright © Cambridge University Press 1992

References

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