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A combined AFLP–multiplex PCR assay for molecular typing of Escherichia coli strains using variable bacterial interspersed mosaic elements

Published online by Cambridge University Press:  27 January 2004

R. HORVÁTH ,
M. DENDIS ,
J. SCHLEGELOVÁ ,
F. RŮŽIČKA  and
J. BENEDÍK
R. HORVÁTH
Affiliation:
Genex CZ Ltd, Podstránská 74, 600 00 Brno, Czech Republic
M. DENDIS
Affiliation:
Genex CZ Ltd, Podstránská 74, 600 00 Brno, Czech Republic
J. SCHLEGELOVÁ
Affiliation:
Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic
F. RŮŽIČKA
Affiliation:
Institute of Microbiology, Medical School, Masaryk University and St Anna University Hospital Brno, Pekařská 53, 635 00 Brno, Czech Republic
J. BENEDÍK
Affiliation:
Genotyp Ltd, Babičkova 116/29, 61300 Brno, Czech Republic
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Abstract

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The original method for molecular typing of E. coli strains was developed using the polymorphism in chromosomal sequences of bacterial interspersed mosaic elements (BIMEs) detected by multiplex PCR and analysed by AFLP assay. The applicability of the method in the epidemiology of E. coli was tested on a group of 524 strains of human and veterinary origin. In the studied group 18 different genotypes were detected. Significant differences were found in the frequencies of the genotypes among various groups of strains, suggesting the method could be a promising tool in the epidemiology of E. coli.

Type
Research Article
Information
Epidemiology & Infection , Volume 132 , Issue 1 , February 2004 , pp. 61 - 65
Copyright
© 2004 Cambridge University Press