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Comparison of serotype, biotype and bacteriocin type with rDNA RFLP patterns for the type identification of Serratia marcescens

Published online by Cambridge University Press:  15 May 2009

R. Alonso ,
H. M. Aucken ,
J. C. Perez-Diaz ,
B. D. Cookson ,
F. Baquero  and
T. L. Pitt
R. Alonso
Affiliation:
Servicio de Microbiología, Hospital Ramon y Cajal, Ctra. Colmenar Km. 9, 1000, 28034 Madrid, Spain
H. M. Aucken
Affiliation:
Laboratory of Hospital Infection, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT, UK
J. C. Perez-Diaz
Affiliation:
Servicio de Microbiología, Hospital Ramon y Cajal, Ctra. Colmenar Km. 9, 1000, 28034 Madrid, Spain
B. D. Cookson
Affiliation:
Laboratory of Hospital Infection, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT, UK
F. Baquero
Affiliation:
Servicio de Microbiología, Hospital Ramon y Cajal, Ctra. Colmenar Km. 9, 1000, 28034 Madrid, Spain
T. L. Pitt*
Affiliation:
Laboratory of Hospital Infection, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT, UK
*
* Author for correspondence.
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Variations in rDNA gene loci in DNA digests of 209 clinical isolates of Serratia marcescens were determined with an Escherichia coli rRNA probe. Forty-one restriction fragment length polymorphism patterns (ribotypes) were identified, based on the size of 4–14 (mean 7·5) hybridization bands. The patterns differed by more than a single band in 98% of pair-wise comparisons. On a subset of 76 isolates, ribotyping proved to be marginally more discriminating than biotyping (discrimination index 0·92 v. 0·89) followed by serotyping (0·87) and bacteriocin typing (0·74). About one-third of isolates belonged to unique ribotypes and only two ribotypes exceeded 5% in frequency (23·0 and 6·4% respectively). A combination of serotype or biotype with ribotyping defined a similar number of strains, although none of the methods alone was sufficiently discriminatory to identify strains. We conclude that due to the accessibility of biotyping and the lack of commercially available antisera for S. marcescens, the biotype and ribotype together provide reliable markers of strain identity.

Type
Research Article
Information
Epidemiology & Infection , Volume 111 , Issue 1 , August 1993 , pp. 99 - 107
Copyright
Copyright © Cambridge University Press 1993

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