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A comparison of the results of the brucellosis radioimmunoassay and other serological tests in experimentally infected cattle

Published online by Cambridge University Press:  25 March 2010

J. Hayes  and
R. J. Chappel
J. Hayes
Affiliation:
Department of Agriculture, Attwood Veterinary Research Laboratory, Westmeadows, Victoria 3047, Australia
R. J. Chappel
Affiliation:
Department of Agriculture, Attwood Veterinary Research Laboratory, Westmeadows, Victoria 3047, Australia
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Summary

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The serology of 27 heifers found to be positive to culture after inoculation with Brucella abortus strain 544, was studied. Eighteen heifers had previously been vaccinated with strain 19 or strain 45/20 and nine were unvaccinated. Post-infection serum samples were tested for Brucella antibodies by radioimmunoassay (RIA), complement fixation test (CFT), indirect haemolysis test (IHLT) and Rose Bengal plate test (RBPT).

All of the unvaccinated heifers showed strong humoral responses to experimental infection in the RIA, CFT, IHLT and RBPT. The CFT and RBPT became positive sooner after infection than the other tests in the unvaccinated heifers. However, in vaccinated heifers the RIA was the most sensitive test early in infection and the results of the RBPT were variable. Three of the vaccinated heifers showed weak and inconsistent humoral responses and, in these animals, the RIA gave fewer false negative reactions than the other tests.

Type
Research Article
Information
Journal of Hygiene , Volume 88 , Issue 1 , February 1982 , pp. 21 - 28
Copyright
Copyright © Cambridge University Press 1982

References

Allan, G. S., Chappel, R. J., Williamson, P. & McNaught, D. J. (1976). A quantitative comparison of the sensitivity of serological tests for bovine brucellosis to different antibody classes. Journal of Hygiene 76, 287.CrossRefGoogle ScholarPubMed
Alton, G. G. (1978). Recent developments in vaccination against bovine brucellosis. Australian Veterinary Journal 54, 551.CrossRefGoogle ScholarPubMed
Alton, G. G., Jones, L. M. & Pietz, D. E. (1975). Laboratory techniques in brucellosis, 2nd ed.World Health Organisation Monograph Series no. 55. Geneva.Google Scholar
Chappel, R. J., Hayes, J., Rogerson, B. A. & Shenfield, L. J. (1981). The serological response of cattle to vaccines against brucellosis, as measured by the brucellosis radioimmunoassay and other tests. Journal of Hygiene 88, 11.CrossRefGoogle Scholar
Chappel, R. J., Williamson, P., McNaught, D. J., Dalling, M. J. & Allan, G. S. (1976). Radioimmunoassay for antibodies against Brucella abortus: a new serological test for bovine brucellosis. Journal of Hygiene 77, 369.CrossRefGoogle ScholarPubMed
Cordes, D. O. & Carter, M. E. (1979). Persistence of Brucella abortus infection in six herds of cattle under brucellosis eradication. New Zealand Veterinary Journal 27, 255.CrossRefGoogle ScholarPubMed
Farrell, I. D. (1974). The development of a new selective medium for the isolation of Brucella abortus from contaminated sources. Research in Veterinary Science 16, 280.CrossRefGoogle ScholarPubMed
Morgan, W. J. B. (1971). Some recent advances in the diagnosis of brucellosis. Irish Veterinary Journal 25, 214.Google Scholar
Nelson, C. J., Anderson, R. K., Kimberling, C. V. & Pietz, D. E. (1966). Epizootiologic factors of bovine brucellosis: Comparative bacteriologic studies of infected herds. American Journal of Veterinary Research 27, 1515.Google ScholarPubMed
Nicoletti, P. & Muraschi, T. F. (1966). Bacteriologic evaluation of serologic test procedures for the diagnosis of brucellosis in problem cattle herds. American Journal of Veterinary Research 27, 689.Google ScholarPubMed
Plackett, P., Cottew, G. S. & Best, S. J. (1976). An indirect haemolysis test (IHLT) for bovine brucellosis. Australian Veterinary Journal 52, 136.CrossRefGoogle ScholarPubMed
Williams, M. R. & Halliday, R. (1980). The relationship between serum immunoglobulin levels and specific antibody production in cows. Research in Veterinary Science 28, 76.CrossRefGoogle ScholarPubMed