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Contribution to the study of live streptomycin–dependent Salmonella vaccines: the problem of reversion to a virulent form

Published online by Cambridge University Press:  15 May 2009

I. R. Vladoianu ,
F. Dubini  and
Adele Bolloli
I. R. Vladoianu
Affiliation:
Institute of Pharmacology, University of Milan, Medical School, Milan, Italy
F. Dubini
Affiliation:
Institute of Pharmacology, University of Milan, Medical School, Milan, Italy
Adele Bolloli
Affiliation:
Institute of Pharmacology, University of Milan, Medical School, Milan, Italy
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The recovery of virulence by means of reversion of a live streptomycin-dependent (Sm D) Salmonella typhimurium vaccine was studied in CD–I Swiss mice. Initially, a one–step Sm D mutant was obtained from a virulent streptomycin-sensitive (Sm S) S. typhimurium strain. Afterwards, two pools of streptomycin-independent (Sm I) revertants were prepared from the Sm D strain. The virulence of the Sm D strain and of the Sm I revertants was tested intraperitoneally. In the virulence testing the original suspension of the Sm I revertants, as well as their 1st and 10th passages on plain medium, medium + 50 μg. streptomycin/ml, and medium + 1000 μg. streptomycin/ml, were used. The results show that the Sm D mutant was avirulent, its avirulence being due to an intrinsic, genetic quality. The Sm I revertants, compared to the original Sm S strain, also displayed a lack of virulence. However, afterwards, the Sm I revertants behaved quite differently, according to their subsequent passages. Indeed, there was an increase in virulence after passages on plain medium, whereas after similar passages on medium containing the drug, the virulence not only failed to increase, but disappeared almost completely. Moreover, the passages on medium containing 1000 μg. streptomycin/ml, induced a return to the status of drug–dependence. The danger of recovery of virulence by means of revertants is evaluated.

Type
Research Article
Information
Journal of Hygiene , Volume 75 , Issue 2 , October 1975 , pp. 203 - 214
Copyright
Copyright © Cambridge University Press 1975

References

REFERENCES

Archer, J. R. & Rawley, D. (1969). A quantitative comparison of the antigenic structure of a virulent and an avirulent strain of Salmonella typhimurium. Immunology 17, 551–8.Google Scholar
Bacon, G. A., Burrows, T. W. & Yates, M. (1951). The effects of biochemical mutations on the virulence of Bacterium typhosum. British Journal of Experimental Pathology 32, 85–96.Google ScholarPubMed
Baron, L. S. & Formal, S. B. (1960). Immunization studies with living vaccine of Salmonella typhimurium. Proceedings of the Society for Experimental Biology and Medicine 104, 565–7.CrossRefGoogle ScholarPubMed
Besredka, A. (1910). De la vaccination par les virus sensibilisés. Bulletin de l'institut Pasteur 8, 241–53.Google Scholar
Besredka, A. (1913). Deux ans de vaccination antityphique avec du virus sensibilisé vivant. Annales de l'institut Pasteur 22, 607–19.Google Scholar
Blanden, R. V., Mackaness, G. B. & Collins, F. M. (1966). Mechanisms of acquired-resistance in mouse typhoid. Journal of Experimental Medicine 124, 585–600.CrossRefGoogle ScholarPubMed
Ciuca, M., Combiescu, D. & Balteanu, J. (1915). Deux épidémies de fièvre typhoide. Vaccinations antityphiques au virus sensibilisé vivant de Besredka. Annales de l'institut Pasteur 29, 105–15.Google Scholar
Cooper, G. N. & Fahey, K. J. (1970). Oral immunization in experimental salmonellosis. III. Behaviour of virulent and temperature–sensitive mutant strains in the intestinal tissues of rats. Infection and Immunity 2, 192–200.CrossRefGoogle ScholarPubMed
Cvjetanovic, B., Mel, D. M. & Felsenfeld, D. (1970). Study of live typhoid vaccine in chimpanzees. Bulletin of the World Health Organization 42, 499–507.Google ScholarPubMed
Du Pont, H. L., Hornick, R. B., Snyder, M. J., Libonati, J. P. & Woodward, T. E. (1970). Immunity in typhoid fever: Evaluation of live streptomycin–dependent vaccine. Antimicrobial Agents and Chemotherapy 10, 236–9.Google ScholarPubMed
Fahey, K. J. & Cooper, C. N. (1970 a). Oral immunization against experimental salmonellosis. I. Development of temperature–sensitive mutant vaccines. Infection and Immunity 1, 263–70.CrossRefGoogle ScholarPubMed
Fahey, K. J. & Cooper, G. N. (1970 b). Oral immunization in experimental salmonellosis. II. Characteristics of the immune response to temperature–sensitive mutants given by oral and parenteral routes. Infection and Immunity 2, 183–91.CrossRefGoogle ScholarPubMed
Felix, A. (1951). The preparation, testing and standardization of typhoid vaccine. Journal of Hygiene 49, 268–87.Google ScholarPubMed
Formal, S. B., La Breo, E. H., Kent, T. H. & Falkow, S. (1965 a). Abortive intestinal infection with an Escherichia coli–Shigella flexneri hybrid strain. Journal of Bacteriology 89, 1374–82.CrossRefGoogle ScholarPubMed
Formal, S. B., La Brec, E. H., Palmer, A. & Falkow, S. (1965 b). Protection of monkeys against experimental shigellosis with attenuated vaccines. Journal of Bacteriology 90, 63–8.CrossRefGoogle ScholarPubMed
Formal, S. B., Kent, T. H., May, H. C., Palmer, A., Falrow, S. & La Brec, E. H. (1966). Protection of monkeys against experimental shigellosis with a living attenuated oral polyvalent dysentery vaccine. Journal of Bacteriology 92, 17–22.CrossRefGoogle ScholarPubMed
Furness, G. & Rowley, D. (1956). Transduction of virulence within the species Salmonella typhimurium. Journal of General Microbiology 15, 140–45.CrossRefGoogle ScholarPubMed
Goldschmidt, E. P., Matney, T. S. & Bausum, H. T. (1962). Genetic analyses of mutations from streptomycin dependence to independence in Salmonella typhimurium. Genetics 47, 1475–87.CrossRefGoogle ScholarPubMed
Hashimoto, K. (1960). Streptomycin resistance in Escherichia coli analysed by transduction. Genetics 45, 49–62.CrossRefGoogle Scholar
Herzberg, M. (1962). Living organisms as immunizing agents against experimental salmonellosis in mice. I. Virulence of auxotrophic mutants. Journal of Infectious Diseases 111, 192–203.CrossRefGoogle Scholar
Hobson, D. (1957). Resistance to reinfection in experimental mouse typhoid. Journal of Hygiene 55, 334–43.CrossRefGoogle ScholarPubMed
Hornick, R. B., Greisman, S. E., Woodward, T. E., Du Pont, H. L., Dowkins, A. T. & Snyder, M. (1970). Medical Progress. Typhoid fever: Pathogenesis and immunological control. New England Journal of Medicine 283, 686–91, 739–46.CrossRefGoogle Scholar
Hornick, R. B. & Du Pont, H. L. (1970). Oral live virus typhoid vaccine tests show promise. Journal of the American medical Association 214, 991.Google Scholar
Istrati, G. (1961). Recherche sur l'immunité active de l'homme dans la dysentérie bacillaire. Note. I. Archives roumaines de pathologie expérimentale et de microbiologie 20, 53–62.Google Scholar
Istrati, G., Meitert, T., Ciufecu, C. & Filipesco, S. (1964). Recherches sur l'immunité active de l'homme dans la dysentérie bacillaire. Note II. Archives Roumaines de Pathologie Expérimentale et de Microbiologie 23, 141–8.Google Scholar
Istrati, G., Meitert, T. & Ciufecu, C. (1965 a). Recherches sur l'immunité active de l'homme dans le dysentérie bacillaire. Note III. Archives Roumaines de Pathologie Expérimentale et de Microbiologie 24, 677–86.Google Scholar
Istrati, G., Istrati, Maria, Meitert, T. & Ciufecu, C. (1965 b). Stabilité génétique du caractère non-pathogène et immunogène antiinfectieux de quelques souches de Sh. flexneri 2a. Archives Roumaines de Pathologie Expérimentale et de Microbiologie 24, 867–74.Google ScholarPubMed
Istrati, G., Meitert, T. & Ciufecu, C. (1965 c). Innocuité de la souche vivante et non-pathogène Shigella flexneri 2a T XXXII administrée par voie souscutanée et intramusculaire à des volontaires. Archives Roumaines de Pathologie Expérimentale et de Microbiologie 24, 875–8.Google Scholar
Istrati, G., Meitert, T. & Ciufecu, C. (1968). Treatment of dysentery bacilli carriers with a live non-pathogenic antidysenteric vaccine. Archivum Immunologiae et Therapiae experimentalis 16, 333–7.Google Scholar
Kenny, K. & Herzberg, M. (1967). Early antibody response in mice to either infection or immunization with Salmonella typhimurium. Journal of Bacteriology 93, 773–8.CrossRefGoogle ScholarPubMed
Kishimoto, Y. (1965). Live vaccine immunization in experimental typhoid with a str-d strain of S. enteritidis. Japanese Journal of Bacteriology 20, 195–202.Google Scholar
Mel, D. M., Terzin, A. L. & Vuksic, L. (1965 a). Studies on vaccination against bacillary dysentery. I. Immunization of mice against experimental shigella infection. Bulletin of the World Health Organization 32, 633–6.Google ScholarPubMed
Mel, D. M., Papo, R. G., Terzin, A. L. & Vuksic, L. (1965 b). Studies on vaccination against bacillary dysentery. 2. Safety tests and reactogenicity studies on a live dysentery vaccine intended for use in field trials. Bulletin of the World Health Organization 32, 637–45.Google ScholarPubMed
Mel, D. M., Terzin, A. L. & Vuksic, L. (1965 c). Studies on vaccination against bacillary dysentery. 3. Effective oral immunization against Shigella flexneri 2a in a field trial. Bulletin of the World Health Organization 32, 647–55.Google Scholar
Mel, D. M., Arsic, B. L., Nicolic, B. D. & Radovanovic, M. L. (1968). Studies on vaccination against bacillary dysentery. 4. Oral immunization with live monotypic and combined vaccines. Bulletin of the World Health Organization 39, 375–80.Google ScholarPubMed
Mel, D. M., Gangarosa, E. J., Radovanovic, M. L., Arsic, B. L. & Litvinjenco, S. (1971). Studies on vaccination against bacillary dysentery. 6. Protection of children by oral immunization with streptomycin-dependent Shigella strains. Bulletin of the World Health Organization 45, 457–64.Google ScholarPubMed
Orskov, J., Jensen, K. A. & Kobayashi, K. (1928). Studien über Breslauinfektion der Mäuse, speziell mit Rücksicht auf die Bedeutung des Reticuloendothelialgewebes. Zeitschrift für Immunitätsforschung und experimentelle Therapie 55, 34–68.Google Scholar
Orskov, J. (1940). Infektionsmechanische Untersuchungen über unspezifische, lokale, gesteigerte bzw. herabgesetzte Resistenz (Promunität. bzw. Mucin). Zeitschrift für Immunitätsforschung und experimentelle Therapie 98, 359–72.Google Scholar
Paine, T. F. & Finland, M. (1948). Observations on bacteria sensitive to, resistant to and dependent upon streptomycin. Journal of Bacteriology 56, 207–18.CrossRefGoogle ScholarPubMed
Reitman, M. (1967). Infectivity and antigenicity of streptomycin-dependent Salmonella typhosa. Journal of Infectious Diseases 117, 101–7.CrossRefGoogle ScholarPubMed
Schewe, Eleonore (1958). Effects of modified host metabolism and altered defense mechanisms on survival time and pathogen counts in tissues and total body of mice infected intravenously with Salmonella typhimurium. Journal of Infectious Diseases 102, 275–93.CrossRefGoogle ScholarPubMed
Schnitzer, J. R. & Grunberg, E. (1957). Change of virulence of drug-resistant bacteria. In Drug Resistance of Microorganisms. Academic Press, Inc., New York, 157–67.Google Scholar
Sergeev, V. V., Taratorina, O. M. & Elkina, S. I. (1967). Pathogenic and immunogenic properties of Streptomycin-dependent mutant of Salmonella gärtneri. Zhurnal Mikrobiologii, Epidemiologii i Immunobiologii 44, 56–60.Google Scholar
Shuster, B. Yu., Sergeev, V. V., Elkina, S. I., Limarev, V. A. & Likhoded, V. G. (1971). Virulent properties of revertants of streptomycin-dependent salmonella mutants. Zhurnal Mikrobiologii, Epidemiologii i Immunobiologii 48, 29–32.Google ScholarPubMed
Vladoianu, I. R., Rusu, V. V., Nas, Ligia & Strejnico, F. (1960). Recherches expérimentales concernant la méthode mixte (orale–souscutanée) de la vaccination antityphoidique. Archives Roumaines de Pathologie Expérimentale et de Microbiologie 19, 137–47.Google Scholar
Vladoianu, I. R. & Dimache, G. (1964). Comportement des souris DBA et H envers l'infection expérimentale avec S. typhimurium. Archives Roumaines de Pathologie Expérimentale et de Microbiologie 23, 59–62.Google ScholarPubMed
Vladoianu, I. R., Dimache, C., Antohi, S., Vladoianu, Constanta & Zarma, Ortansa (1965). Laboratory tests on the effectiveness of oral vaccination of young children against typhoid and paratyphoid A and B. Bulletin of the World Health Organization 32, 37–45.Google ScholarPubMed
Vladoianu, I. R. & Dimache, G. (1965). Contributions to the study of oral antityphoid vaccination. Microbiologia, parazitologia si epidemiologia (Bucarest) 10, 97–102.Google Scholar
World Health Organization (1954). Expert Committee on Biological Standardization. Technical report Series No. 86, 14.Google Scholar
World Health Organization (1972). Oral Enteric Bacterial Vaccines. Report of a WHO Scientific Group. Technical report Series, No. 500.Google Scholar