Hostname: page-component-7479d7b7d-rvbq7 Total loading time: 0 Render date: 2024-07-12T22:23:19.289Z Has data issue: false hasContentIssue false
  • English
  • Français

The direct fluorescent antibody test for detection of Brucella abortus in bovine abortion material

Published online by Cambridge University Press:  15 May 2009

M. J. Corbel
M. J. Corbel
Affiliation:
Ministry of Agriculture, Fisheries and Food, Central Veterinary Laboratory, Weybridge, Surrey
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The direct fluorescent antibody test was assessed for detection of Brucella abortus in bovine abortion material. Br. abortus organisms could be readily detected as characteristic intra-cellular clumps, in smears stained with fluorescent antibody. Counterstaining with Evans Blue was necessary to suppress tissue auto-fluorescence. The method was specific and enabled brucella infection to be differentiated, inter alia, from Q-fever infection.

Type
Research Article
Information
Journal of Hygiene , Volume 71 , Issue 1 , March 1973 , pp. 123 - 129
Copyright
Copyright © Cambridge University Press 1973

References

REFERENCES

Ahvonen, P., Jansson, E. & Aho, K. (1969). Marked cross-agglutination between Brucellae and a sub-type of Yersinia enterocolitica. Acta pathologica et microbiologica scandinavica 75, 291.Google Scholar
Biegeleisen, J. Z. Jr, Moody, M. D., Marcus, B. B. & Flynt, J. W. (1962). The use of fluorescein-labeled anti-Brucella suis globulin for demonstrating Brucella antigen in animal tissues. American Journal of Veterinary Research 23, 592.Google Scholar
Cherry, W. B., Goldman, M., Carski, T. R. & Moody, M. D. (1960). In Fluorescent Antibody Techniques in the Diagnosis of Communicable Diseases. U.S. Department of Health, Education and Welfare, Atlanta, Ga.Google Scholar
Chistov, N. P., Pesina, A. G. & Voronova, L. A. (1961). Primeneniye fluorestsiruyushchikh antitel dlya vyyavleniya brutsell. Sbornik trudov Leningradskiy nauchno-issledovatel'skiy Veterinarnyy Institut 9, 9.Google Scholar
Christoffersen, P. A. & Ottosen, H. E. (1941). Recent staining methods. Skandinavisk veterinärtidskrift 31, 599.Google Scholar
Corbel, M. J. & Cullen, G. A. (1970). Differentiation of the serological response to Yersinia enterocolitica and Brucella abortus in cattle. Journal of Hygiene 68, 519.Google ScholarPubMed
Cullen, G. A. & Corbel, M. J. (1970). Observations on some possible causes of variation in the titre of brucella antibodies in cattle. Veterinary Record 87, 101.CrossRefGoogle ScholarPubMed
Forsgren, A. & Sjöquist, J. (1967). ‘Protein A’ from Staphylococcus aureus. III. Reaction with rabbit γ-globulin. Journal of Immunology 99, 19.CrossRefGoogle Scholar
Ignat'eva, O. A. (1961). Uskorennoye opredeleniye brutsell v organizme zhivotnykh v period inkubatsii s ispol'zovaniem mechenykh izotsionat fluorestseinom antitel. Uchenyye Zapiski Kazanskogo veterinarnogo Instituta 81, 57.Google Scholar
Ignat'eva, O. A. (1962). Uskorennoye opredeleniye brutsell v organizme zhivotnykh v ranney stadii inkubatsii s primeneniyem fluorestsiruyushchikh antitel. Uchenyye Zapiski Kazanskogo Veterinarnogo Instituta 84, 59.Google Scholar
Janney, G. C. & Berman, D. T. (1962). Staining intracellular Brucella organisms by means of fluorescent antibodies. American Journal of Veterinary Research 23, 596.Google ScholarPubMed
Keckwick, R. A. (1940). The serum proteins in multiple myelomatosis. Biochemical Journal 34, 1248.CrossRefGoogle Scholar
Lapraik, R. D., MacKinnon, D. J. & Slavin, G. (1967). Bovine abortion: possible confusion of Br. abortus and ‘Q’ fever infections. Scientific letter. Veterinary Record 81, 414.Google Scholar
Meyer, M. (1966). Identification of Brucella organisms by immunofluorescence. American Journal of Veterinary Research 27, 424.Google ScholarPubMed
Moody, M. D., Biegeleisen, J. Z. Jr & Taylor, G. C. (1961). Detection of brucellae and their antibodies by fluorescent antibody and agglutination tests. Journal of Bacteriology 81, 990.CrossRefGoogle ScholarPubMed
Morris, C. J. O. R. (1964). Thin layer chromatography of proteins on Sephadex G-100 and G-200. Journal of Chromatography 16, 167.CrossRefGoogle ScholarPubMed
Moulton, J. E. & Meyer, M. E. (1958). The pathogenesis of Brucella suis infection in guinea pigs. Lesions of the spleen, liver, testis and articulations. Cornell Veterinarian 48, 165.Google ScholarPubMed
Schweizer, R. (1964). Bang-oder Q-fever Abort. Schweizer Archiv für Tierheilkunde 106, 213.Google Scholar
The, T. H. & Feltkamp, T. E. W. (1970). Conjugation of fluorescein isothiocyanate to antibodies. 1. Experiments on the conditions of conjugation. Immunology 18, 865.Google Scholar