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Evaluation and use of the drug inhibition method of measuring intracellular killing in differentiating between staphylococci grown in vivo and in vitro

Published online by Cambridge University Press:  19 October 2009

D. O. Kolawole
D. O. Kolawole
Affiliation:
Department of Microbiology, University of Ife, Ile-Ife, Nigeria
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Summary

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A Staphylococcus aureus strain grown once in vivo in rabbit pleural cavities was appreciably more resistant to killing by soluble polymorph bactericidins than its broth-grown counterpart. Parallel results were obtained using an adaptation of Solberg's drug-inhibition method of measuring intracellular killing after critical aspects of it had been evaluated and modified. Individual in vivo grown organisms were surrounded by a layer of less dense material which was not capsular in nature but which made the organisms clump together. This surface coating was lost on subculture in vitro, resulting in a reversion to broth-like susceptibility, thus indicating that the surface coating was largely responsible for the enhanced resistance and that it resulted from interactions between staphylococci and components of the rabbits' natural body fluids. Consequently, growth in plasma produced organisms which mimicked in vivo grown organisms in clumping, surface coating and in resistance to killing. The use of plasma-grown staphylococci in further studies of likely resistance mechanisms in vivo is discussed.

Type
Research Article
Information
Journal of Hygiene , Volume 91 , Issue 2 , October 1983 , pp. 211 - 222
Copyright
Copyright © Cambridge University Press 1983

References

REFERENCES

Adlam, C., Pearce, J. H. & Smith, H. (1970 a). Virulence mechanisms of staphylococci grown in vivo and in vitro. Journal of Medical Microbiology 3, 147–155.CrossRefGoogle ScholarPubMed
Adlam, C., Pearce, J. H. & Smith, H. (1970 b). The interaction of staphylococci grown in vivo and in vitro with polymorphonuclear leukocytes. Journal of Medical Microbiology 3, 157–103.CrossRefGoogle Scholar
Alexander, J. W. & Goon, R. A. (1968). Effect of antibiotics on the bactericidal activity of human leukocytes. Journal of laboratory and Clinical Medicine 71. 971–983.Google ScholarPubMed
Beining, D. R. & Kennedy, E. R. (1963). Characteristics of a strain of Staphylococews aureus grown in I'iro and hi I'itro. Journal of Bacteriology 85. 732–741.CrossRefGoogle Scholar
Cohn, Z. A. & Hirsch, J. G. (1960). The isolation and properties of the specific cytoplasmic granules of rabbit polyinorphonuelear leukocytes. Journal of Experimental Medicine 112, 983–1004.CrossRefGoogle Scholar
Cohn, Z. A. & Morse, S. I. (1959). Interactions between rabbit polymorphonuclear leukocytes and staphylococci. Journal of Experimental Medicine 110, 410–443.CrossRefGoogle ScholarPubMed
Craig, C. P. & Suter, E. (1966). Extracellular factors influencing staphylocidal capacity of human polymorphonuclear leukocytes. Journal of Immunology 97, 287–200.CrossRefGoogle ScholarPubMed
Duthie, E. S. (1954). Evidence for two forms of staphylococcal coagulase. Journal of General Microbiology 10, 427–430.CrossRefGoogle ScholarPubMed
Ekstedt, R. D. & Beknhard, J. M. (1973). Preparation and characterization of a slime layer material produced by Staphylococcus aureus. Proceedings of the Society for Exjierimental Biology and Medicine 142, 86–91.CrossRefGoogle ScholarPubMed
Gellenheck, S. M. (1962). Aerobic respiratory mechanism of Staphylococctis aureus from an infected animal. Journal of Bacteriology 83, 450–455.CrossRefGoogle Scholar
Gladstone, G. P. & Glencross, E. J. G. (1960). Growth and toxin production of staphylococci in cellophane sacs in vivo. British Journal of Experimental Pathology 41, 313–333.Google ScholarPubMed
Gladstone, G. P., Walton, E. & Kay, U. (1974). The effect of cultural conditions on the susceptibility of staphylococci to killing by the cationic proteins from rabbit polymorphonuclear leukocytes. British Journal of Experimental Pathology 55, 427—147.Google Scholar
Holmes, B.Quie, P. G., Windiiurst, D. B., Pollaua, B. & Good, R. A. (1966). Protection of phagocytized bacteria from the killing action of antibiotics. Nature, London 210, 1131–1132.CrossRefGoogle ScholarPubMed
Solberg, C. O. (1972 a). Evaluation of neutrophil granulocyte functions. Ada Pathologica et Microhiologica Scandinavica B80, 550–563.Google Scholar
Solbero, C. O. (1972 b). Protection of phagocytized bacteria against antibiotics. Ada Medica Scandinavica 191, 383–387.Google Scholar
Tan, J. S., Watanakunakorn, C. & Phair, J. P. (1971). A modified assay of neutrophil function: Use of lysostaphin to differentiate defective phagocytosis from impaired intracellular killing. Journal of Laboratory and Clinical Medicine 78, 316–322.Google ScholarPubMed