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Further experiments on the adaptation of Mahoney, Type 1, poliomyelitis virus to suckling mice

Published online by Cambridge University Press:  15 May 2009

Golda Selzer  and
M. Butchart
Golda Selzer
Affiliation:
C.S.I.R. and U.C.T. Virus Research Unit, Department of Pathology, University of Cape Town, South Africa
M. Butchart
Affiliation:
C.S.I.R. and U.C.T. Virus Research Unit, Department of Pathology, University of Cape Town, South Africa
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The Mahoney, Type 1, poliomyelitis virus had been through six to-and-fro monkey kidney-tissue culture and suckling mouse passages. Early paralysis was produced in groups of mice inoculated directly from infected tissue-culture fluid, but mouse-to-mouse passage failed to produce paralysis except in an occasional mouse. At the 7th tissue-culture mouse passage, the virus harvested from paralysed mice was inoculated into 100 mice. One mouse developed paralysis within 24 hr. From this mouse a variant of Mahoney virus was isolated which has now been through seventy-five mouse-to-mouse passages and produces paralysis in 90–100% of mice within 3 days.

The authors would like to express their deep appreciation of Prof. A. Kipps' constant interest and encouragement.

Type
Research Article
Information
Journal of Hygiene , Volume 57 , Issue 3 , September 1959 , pp. 285 - 290
Copyright
Copyright © Cambridge University Press 1959

References

REFERENCES

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