Hostname: page-component-848d4c4894-pftt2 Total loading time: 0 Render date: 2024-06-09T17:01:54.623Z Has data issue: false hasContentIssue false
  • English
  • Français

The haemagglutinins of influenza A (H1N1) viruses in the ‘O’ or ‘D’ phases exhibit biological and antigenic differences

Published online by Cambridge University Press:  15 May 2009

A. Azzi ,
O. Bartolomei-Corsi ,
K. Zakrzewska ,
T. Corcoran ,
R. Newman ,
J. S. Robertson ,
P. Yates  and
J. S. Oxford
A. Azzi
Affiliation:
Institute of Microbiology, The University, Florence, Italy
O. Bartolomei-Corsi
Affiliation:
Institute of Microbiology, The University, Florence, Italy
K. Zakrzewska
Affiliation:
Institute of Microbiology, The University, Florence, Italy
T. Corcoran
Affiliation:
National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Herts EN6 3QG
R. Newman
Affiliation:
National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Herts EN6 3QG
J. S. Robertson
Affiliation:
National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Herts EN6 3QG
P. Yates
Affiliation:
National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Herts EN6 3QG
J. S. Oxford*
Affiliation:
Department of Academic Virology, The London Hospital Medical College, Whitechapel, London E1 2AD
*
* Author for correspondence.
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Influenza A (H1N1) viruses when initially isolated in mammalian cell cultures (MDCK cells) had different agglutination reactions with chicken and guinea-pig erythrocytes compared to the same viruses after passage. On first isolation the virus HA resembled the ‘O’ phase viruses described originally by Burnet and Bull and agglutinated mammalian but not avian erythrocytes. After passage, the virus HA resembled a classical ‘D’ phase virus and agglutinated both avian and mammalian erythrocytes. Monoclonal and polyclonal antisera detected antigenic differences between the HAs of the viruses in the ‘O’ and ‘D’ phases. The ‘O’ phase virus HA reacted preferentially with antibodies in post infection human antisera. Viruses in the ‘O’ phase replicated poorly in the allantoic cavity of embryonated hens' eggs whilst ‘D’ phase virus replicated in both MDCK cells and in embryonated hens' eggs. At least three distinguishable subpopulations of influenza A (H1N1) viruses may co-exist in clinical throat swab material, including viruses possessing HAs in the ‘O’ and ‘D’ phases and other ‘D’ phase viruses cultivable in embryonated hens' eggs but antigenically distinguishable from the corresponding ‘D’ phase virus in MDCK cells.

Type
Research Article
Information
Epidemiology & Infection , Volume 111 , Issue 1 , August 1993 , pp. 135 - 142
Copyright
Copyright © Cambridge University Press 1993

References

1.Burnet, FM, Bull, DR. Changes in influenza virus associated with adaptation to passage in chick embryos. J Exp Biol Med Sci 1943; 21: 55–69.CrossRefGoogle Scholar
2.Oxford, JS, Schild, GC. The Orthomyxoviridae and influenza. In: Collier, LH, Timbury, MC, eds. Topley and Wilson's principles of bacteriology, virology and immunology. 8th ed, vol. 4. London: Edward Arnold, 1990; 291–322.3.Google Scholar
3.Schild, GC, Oxford, JS, De Jong, JC, Webster, RG. Evidence for host-cell selection of influenza virus antigenic variants. Nature 1983; 303: 706–9.CrossRefGoogle ScholarPubMed
4.Oxford, JS, Corcoran, T, Knott, R, et al. Serological studies with influenza A (H1N1) viruses cultivated in eggs or canine kidney cell line (MDCK). Bull WHO 1987; 65: 181'7.Google ScholarPubMed
5.Robertson, JS, Nicholson, C, Bootman, JS, Major, D, Robertson, EW, Wood, JM. Sequence analysis of the haemagglutinin of influenza A (H1N1) viruses present in clinical material and comparison with the HA of laboratory derived virus. J Gen Virol 1991; 72: 671–7.CrossRefGoogle Scholar
6.Katz, JM, Webster, RG. Antigenic and structural characterisation of multiple sub-populations of H3N2 influenza virus from an individual. Virology 1988; 165: 446–56.CrossRefGoogle Scholar
7.Katz, JH, Wang, M, Webster, RG. Direct sequencing of the HA gene of influenza (H3N2) virus in original clinical samples reveals sequence identify with mammalian cell-grown virus. J Virol 1990; 64: 1808–11.CrossRefGoogle Scholar
8.Rajakumar, A, Swerkosz, FM, Schulze, IT. Sequence of an influenza virus haemagglutinin determined directly from a clinical sample. Proc Nat Acad Sci USA 1990; 87: 4154–8.CrossRefGoogle ScholarPubMed
9.Wang, M, Katz, JM, Webster, RG. Extensive heterogeneity in the haemagglutinin of egg-grown influenza viruses from different patients. Virology 1989; 171: 275–9.CrossRefGoogle ScholarPubMed
10.Rogers, GN, Paulson, JC, Daniels, RS, Skehel, JJ, Wilson, JA, Wiley, DC. Single amino acid substitutions in influenza haemagglutinin change receptor binding specificity. Nature 1983; 304: 76–8.CrossRefGoogle ScholarPubMed
11.Katz, JM, Naeve, CW, Webster, RG. Host cell-mediated variation in H3N2 influenza viruses. Virology 1987; 56: 386–95.CrossRefGoogle Scholar
12.Patterson, S, Oxford, JS. Analysis of antigenic determinants of internal and external proteins of influenza viruses and identification of antigenic subpopulations of viruses in recent field isolates using monoclonal antibodies and immunogold labelling. Arch Virol 1986; 88: 189–202.CrossRefGoogle ScholarPubMed
13.Nobusawa, F, Nakajima, K. Amino acid substitution at position 226 of the haemagglutinin molecule of influenza (H1N1) virus effects receptor binding activity but not fusion activity. Virology 1988; 167: 8–14.CrossRefGoogle Scholar
14.Aytay, S, Schulze, JT. Single amino acid substitutions in the haemagglutinin can alter the host range and receptor binding properties of H1 strains of influenza A virus. J Virol 1991; 65: 3022–28.CrossRefGoogle ScholarPubMed
15.Robertson, JS, Naeve, CW, Webster, RG, Bootman, JS, Newman, R, Schild, GC. Alteration of haemagglutinin associated adaptation of influenza B virus to growth in eggs. Virology 1985; 143: 166–74.CrossRefGoogle ScholarPubMed
16.Kohler, G, Milstein, C. Continuous culture of fused cells secreting antibody of pre-defined specificity. Nature 1975; 256: 495–7.CrossRefGoogle Scholar
17.Oxford, JS, Corcoran, T, Schild, GC. Naturally occurring ts influenza A viruses of the H1N1 and H3N2 antigenic subtypes. J Gen Virol 1980; 48: 383–9.CrossRefGoogle Scholar
18.Krystal, M, Elliott, RM, Benz, EW, Young, JF, Palese, P. Evolution of influenza A and B viruses: conservation of structural features in the haemagglutinin genes. Proc Natl Acad Sci USA 1982; 79: 4800–4.CrossRefGoogle Scholar
19.Yates, PJ, Bootman, JS, Robertson, JS. The antigenic structure of a human influenza A (H1N1) virus isolate grown exclusively in MDCK cells. J Gen Virol 1990; 71: 1683–8.CrossRefGoogle ScholarPubMed
20.Caton, AJ, Brownlea, GG, Yewdell, JW, Gerhard, W. The antigenic structure of the influenza virus A/PR/78/34 haemagglutinin (H1 subtype). Cell 1982; 31: 417–27.CrossRefGoogle Scholar
21.Oxford, JS, Schild, GC, Corcoran, T, et al. A host cell selected variant of influenza B virus with a single nucleotide substitution in HA affecting a potential glycosylation site was attenuated in virulence for volunteers. Arch Virol 1990; 110: 37–46.CrossRefGoogle Scholar