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Histological study of adenovirus type 14 development in cell cultures

Published online by Cambridge University Press:  15 May 2009

E. Măgureanu ,
M. Muşetescu  and
M. Grobnicu
E. Măgureanu
Affiliation:
This investigation was carried out in the ‘Dr I. Cantacuzino’ Institute, Bucharest
M. Muşetescu
Affiliation:
This investigation was carried out in the ‘Dr I. Cantacuzino’ Institute, Bucharest
M. Grobnicu
Affiliation:
This investigation was carried out in the ‘Dr I. Cantacuzino’ Institute, Bucharest
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By means of the fluorescent antibody method and of acridine orange staining experimental infection with adenovirus type 14 has been investigated in KB and MK cell cultures. Intracellular changes could be shown to precede the appearance of the cytopathic effect.

Thus, progressive stages in the development of adenovirus at this level could be separated both by the demonstration of the specific antigen and by the appearance of an altered DNA.

Both methods have proved to be equally useful for the detection of virus multiplication and of the histochemical changes induced in the cells.

No significant differences have been found to exist with respect to the cell line used.

Type
Research Article
Information
Journal of Hygiene , Volume 63 , Issue 1 , March 1965 , pp. 99 - 104
Copyright
Copyright © Cambridge University Press 1965

References

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