Hostname: page-component-84b7d79bbc-g5fl4 Total loading time: 0 Render date: 2024-07-26T17:31:35.017Z Has data issue: false hasContentIssue false
  • English
  • Français

Human isolates of apramycin-resistant Escherichia coli which contain the genes for the AAC(3)IV enzyme

Published online by Cambridge University Press:  19 October 2009

J. E. B. Hunter ,
C. A. Hart ,
J. C. Shelley ,
J. R. Walton  and
M. Bennett
J. E. B. Hunter
Affiliation:
Departments of Veterinary Clinical Science, University of Liverpool, PO Box 147, Liverpool L69 3BX Medical Microbiology, University of Liverpool, PO Box 147, Liverpool L69 3BX
C. A. Hart
Affiliation:
Medical Microbiology, University of Liverpool, PO Box 147, Liverpool L69 3BX
J. C. Shelley
Affiliation:
Departments of Veterinary Clinical Science, University of Liverpool, PO Box 147, Liverpool L69 3BX
J. R. Walton
Affiliation:
Departments of Veterinary Clinical Science, University of Liverpool, PO Box 147, Liverpool L69 3BX
M. Bennett
Affiliation:
Departments of Veterinary Clinical Science, University of Liverpool, PO Box 147, Liverpool L69 3BX
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Gentamicin-resistant Escherichia coli isolated at different periods from patients in two hospitals were tested for resistance to the aminoglycoside antibiotic apramycin. Twenty-four of 93 (26%) gentamicin-resistant isolates collected from the Royal Liverpool Hospital between 1981 and 1990 were resistant to apramycin. Thirteen isolates were highly resistant to apramycin (minimal inhibitory concentration (MIC) ≥ 1024 μg/ml). were also resistant to gentamicin, netilmicin and tobramycin, and hybridized with a DNA probe derived from the aminoglycoside acetyltransferase (3)IV (AAC(3)IV) gene. The proportion of gentamicinresistant isolates which had high level resistance to apramycin increased from 7% in 1981–5 to 24% in 1986–90.

Twelve gentamicin-resistant E. coli from Guy's and St Thomas's Hospital isolated between 1977 and 1980 were also tested for resistance to apramycin. For five of these isolates the MICs of apramycin was 32–256 μg/ml. None was shown to have a conjugative plasmid carrying resistance to apramycin and only one hybridized with the DNA probe for the AAC(3)IV enzyme.

Type
Research Article
Information
Epidemiology & Infection , Volume 110 , Issue 2 , April 1993 , pp. 253 - 259
Copyright
Copyright © Cambridge University Press 1993

References

REFERENCES

1.Wray, C, Hedges, RW, Shannon, KP, Bradley, DE.Apramycin and gentamicin resistance in Escherichia coli and salmonellas isolated from farm animals. J Hyg 1986; 97: 445–50.Google ScholarPubMed
2.Chaslus-Dancla, E, Gerbaud, G, Lafont, JP, Martel, J, Courvalin, P.Nucleic acid hybridisation with a probe specific for 3-aminoglycoside acetyltransferase type IV: a survey of resistance to apramycin and gentamicin in animal strains of Escherichia coli. FEMS Microbiol Lett 1986; 34: 265–8.Google Scholar
3.Hunter, JEB, Shelley, JC, Walton, JR, Hart, CA, Bennett, M.Apramycin resistance plasmids in Escherichia coli: possible transfer to Salmonella typhimurium in calves. Epidemiol Infect 1992; 108: 271–8.Google ScholarPubMed
4.Chaslus-Dancla, E, Glupczynski, Y, Gerbaud, G, Lagorce, M, Lafont, JP, Courvalin, P.Detection of apramycin resistant Enterobacteriaceae in hospital isolates. FEMS Microbiol Lett 1989; 61: 261–6.Google Scholar
5.Gomez-Lus, R, Rivera, MJ, Gomez-Lus, ML et al. Resistance to apramycin in two enterobacterial clinical isolates: detection of a 3-N-aeetyltransferase IV. J Chemother 1990; 12: 241–3.Google Scholar
6.Threlfall, EJ, Rowe, B, Ferguson, JL, Ward, LR.Characterization of plasmids conferring resistance to gentamicin and apramycin in strains of Salmonella typhimurium phage type 204c isolated in Britain. J Hyg 1986; 97: 419–26.Google ScholarPubMed
7.Johnson, AP, Woodford, N, Threlfall, EJ, George, RC.Resistance to a veterinary aminoglycoside antibiotic (apramycin) causing cross resistance to gentamicin in human clinical isolates of Enterobacteriaceae. J Med Micro 1989; 28: xvi–xvii.Google Scholar
8.Threlfall, EJ, Rowe, B, Ferguson, JL, Ward, LR.Increasing incidence of resistance to gentamicin and related aminoglycosides in Salmonetla typhimurium phage type 204c in England, Wales and Scotland. Vet Rec 1985; 117: 355–7.Google ScholarPubMed
9.Hart, CA.Nosocomial gentamicin- and multiply-resistant enterobacteria at one hospital. Factors associated with carriage. J Hosp Inf 1982; 3: 165–72.Google ScholarPubMed
10.Stokes, EJ, Waterhouse, PM.Antibiotic sensitivity tests by diffusion methods. Assoc Clin Pathol Broadsheets 1973; 55: 1–12.Google Scholar
11.Kudo, CL, Liu, ST.Rapid procedure for detection and isolation of large and small plasmids. J Bacteriol 1981; 145: 1365–73.Google Scholar
12.Bran, B, Pilz, U, Piepersberg, W.Genes for gentamicin-(3)-acetyltransferases 111 and IV: 1. Nucleotiode sequence of the AAC(3)-lV gene and possible involvement of an 1S140 element in its expression. Mol Gen Genet 1984; 193: 179–87.Google Scholar
13.Salauze, D, Ital, I, Gomez-Lus, R, Davies, J.Aminoglyeoside aeetyltransferase 3-1V (aaeC4) and hygroinyoin B4-1 Phosphotransferase (hphB) in bacteria isolated from human and animal sources. Antimicrob Agents Chemother 1990: 34: 1915–20.Google Scholar
14.Chaslus-Danela, E, Gerbaud, G, Martel, JL, Lagorce, M, Lafont, JP, Courvalin, P.Probable transmission between animals of a plasmid encoding aminoglyeoside 3-N-aeetyltransferase IV and dihydrofolate reduetase 1. Vet Microbiol 1987; 15: 97–104.Google Scholar