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Immunogenicity of experimental trachoma vaccines in baboons: I. Experimental methods, and preliminary tests with vaccines prepared in chick embryos and in HeLa cells

Published online by Cambridge University Press:  15 May 2009

L. H. Collier  and
W. A. Blyth
L. H. Collier
Affiliation:
Medical Research Council Trachoma Research Unit, Lister Institute of Preventive Medicine, London, S. W. 1
W. A. Blyth
Affiliation:
Medical Research Council Trachoma Research Unit, Lister Institute of Preventive Medicine, London, S. W. 1
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Parallel titrations of a strain of trachoma (MRC–221) and one of inclusion conjunctivitis (MRC–4) in the baboon conjunctiva and in chick embryos suggest that ten to twenty 50% egg infective doses are equivalent to one 50% baboon infective dose; but that at least 1000 egg infective doses are needed to induce moderate or severe infections in all of a given number of baboons.

For vaccine experiments in baboons, a system of scoring physical signs and presence of inclusion bodies was devised; the significance of differences in vaccinated and control animals in their response to conjunctival challenge was determined by analysis of variance. An aqueous suspension of live MRC–4 grown in the yolk sac was given as two subcutaneous doses and one intravenous dose at weekly intervals, and protected all of six baboons challenged with the homologous strain; three similarly spaced subcutaneous doses were less effective. The immunity induced by this vaccine waned considerably during the ensuing 15 months. Vaccine prepared from a live ‘fast-killing’ variant of MRC–4 grown in HeLa cells was less effective than MRC–4 itself in protecting baboons against infection with the parent strain.

Although both yolk sac and HeLa cell vaccines induced the formation of antibody fixing complement with trachoma group antigen, the serum titres in individual animals at the time of challenge were unrelated to the degree of protection; during a 15 month observation period there were pronounced falls in the titres of antibody induced either by vaccination or by challenge with egg-grown TRIC agent.

We wish to thank Dr I. Sutherland (M.R.C. Statistical Research Unit) for his helpful advice during the early stages of this work. We are also greatly indebted to Mr P. Avis (Pfizer Ltd.) for his advice and for undertaking the statistical computations; and to Miss Anne Smith and Miss Pay Storey (M.R.C. Trachoma Research Unit) for doing the complement fixation tests.

Type
Research Article
Information
Journal of Hygiene , Volume 64 , Issue 4 , December 1966 , pp. 513 - 528
Copyright
Copyright © Cambridge University Press 1966

References

REFERENCES

Bell, S. D., Murray, E. S., Carroll, T. J. & Snyder, J. C. (1963). Studies on trachoma. III. Comparison of virus infectivity for man and chick embryos. Am. J. trop, Med. Hyg. 12, 902.CrossRefGoogle ScholarPubMed
Bietti, G. B., Guerra, P., Felici, A. & Vozza, R. (1962). Research studies on trachoma. Acta Soc. ophthal. jap. 66, 362.Google Scholar
Blyth, W. A., Reeve, P., Graham, D. M. & Taverne, J. (1962). The production of antisera that neutralize inclusion blennorrhoea virus. Br. J. exp. Path. 43, 340.Google ScholarPubMed
Bovarnick, M. R., Miller, J. C. & Snyder, J. C. (1950). The influence of certain salts, amino acids, sugars and proteins on the stability of rickettsiae. J. Bact. 59, 509.CrossRefGoogle ScholarPubMed
Collier, L. H. (1961). Experiments with trachoma vaccines: experimental system using inclusion blennorrhoea virus. Lancet, i, 795.CrossRefGoogle Scholar
Collier, L. H. (1962). Experimental infection of baboons with inclusion blennorrhea and trachoma. Ann. N.Y. Acad. Sci. 98, 188.CrossRefGoogle ScholarPubMed
Collier, L. H. (1963). Nomenclature of isolates of virus from trachoma and inclusion blennorrhoea. Nature, Lond. 198, 1229.CrossRefGoogle ScholarPubMed
Collier, L. H. (1966). The present status of trachoma vaccination studies. Bull. Wld Hlth Org. 34, 233.Google ScholarPubMed
Collier, L. H., Duke-Elder, S. & Jones, B. R. (1958). Experimental trachoma produced by cultured virus. Br. J. Ophthal. 42, 705.CrossRefGoogle ScholarPubMed
Collier, L. H., Duke-Elder, S. & Jones, B. R. (1960). Experimental trachoma produced by cultured virus. Part II. Br. J. Ophthal. 44, 65.CrossRefGoogle ScholarPubMed
Collier, L. H., Sowa, S., Sowa, J. & Blyth, W. A. (1963). Experiments with trachoma vaccines: therapeutic effect on established trachoma. Orient. Archs Ophthal. 1, 67.Google Scholar
Dawson, C., Jawetz, E., Thygeson, P. & Hanna, L. (1961). Trachoma viruses isolated in the United States. 4. Infectivity and immunogenicity for monkeys. Proc. Soc. exp. Biol. Med. 106, 898.CrossRefGoogle ScholarPubMed
Dulbecco, R. & Vogt, M. (1954). Plaque formation and isolation of pure lines with poliomyelitis viruses. J. exp. Med. 99, 167.CrossRefGoogle ScholarPubMed
Fulton, F. & Dumbell, K. R. (1949). The serological comparison of strains of influenza virus. J. gen. Microbiol. 3, 97.CrossRefGoogle ScholarPubMed
Furness, G. & Fraser, E. F. (1962). One-step growth curves for inclusion blennorrhoea virus in HeLa cell monolayers. J. gen. Microbiol. 27, 299.CrossRefGoogle ScholarPubMed
Furness, G., Graham, D. M. & Reeve, P. (1960). The titration of trachoma and inclusion blennorrhoea viruses in cell cultures. J. gen. Microbiol. 23, 613.CrossRefGoogle ScholarPubMed
Gear, J. H. S., Gordon, F. B., Jones, B. R. & Bell, S. D. (1963). Nomenclature of isolates of virus from trachoma and inclusion blennorrhoea. Nature, Lond. 197, 26.CrossRefGoogle Scholar
Graham, D. M. (1965). Growth and neutralization of the trachoma agent in mouse lung. Nature, Lond. 207, 1379.CrossRefGoogle Scholar
Grayston, J. T., Wang, S. P., Woolridge, R. L., Yang, Y. F, & Johnston, P. B. (1960). Trachoma: studies of etiology, laboratory diagnosis and prevention. J. Am. med. Ass. 172, 1577.CrossRefGoogle ScholarPubMed
Grayston, J. T., Woolridge, R. L., Wang, S. P., Yen, C. H., Yang, C. Y., Cheng, K. H. & Chang, I. H. (1963). Field studies of protection from infection by experimental trachoma virus vaccine in preschool-aged children in Taiwan. Proc. Soc. exp. Biol. Med. 112, 589.CrossRefGoogle Scholar
Jones, B. R. (1961). TRIC virus infections in London. Trans, ophthal. Soc. U.K. 81, 367.Google Scholar
Jones, B. R. & Collier, L. H. (1962). Inoculation of man with inclusion blennorrhoea virus. Ann. N.Y. Acad. Sci. 98, 212.CrossRefGoogle Scholar
Jones, B. R., Collier, L. H. & Smith, C. H. (1959). Isolation of virus from inclusion blennorrhoea. Lancet, i, 902.CrossRefGoogle Scholar
Khaw, O. K., Lin, H. M., Wang, S. P., Woolridge, R. L. & Grayston, J. T. (1963). Trachoma vaccine studies in volunteer students of the National Defense Medical Center. III. Oil adjuvant vaccine: antibody response study and eye challenge inoculation with egg grown and purified trachoma virus. Chin. med. J. free China Edn, 10, 97.Google Scholar
Murray, E. S., Bell, S. D., Hanna, A. T., Nichols, R. L. & Snyder, J. C. (1960). Studies on trachoma. I. Isolation and identification of strains of elementary bodies from Saudi Arabia and Egypt. Am. J. trop. Med. Hyg. 9, 116.CrossRefGoogle ScholarPubMed
Reed, L. J. & Muench, H. (1938). A simple method of estimating 50% end points. Am. J. Hyg. 27, 493.Google Scholar
Reeve, P. & Taverne, J. (1962). Some properties of the complement-fixing antigens of the agents of trachoma and inclusion blennorrhoea and the relationship of the antigens to the developmental cycle. J. gen. Microbiol. 27, 501.CrossRefGoogle Scholar
Reeve, P. & Taverne, J. (1963). Observations on the growth of trachoma and inclusion blennorrhoea virus in embryonate eggs. J. Hyg., Camb. 61, 67.CrossRefGoogle Scholar
T'ang, F. F., Chang, H. L., Huang, Y. T. & Wang, K. C. (1957). Studies on the etiology of trachoma with special reference to isolation of the virus in chick embryos. Chin. med. J. 75, 429.Google Scholar
Taverne, J., Blyth, W. A. & Reeve, P. (1964 a). Toxicity of the agents of trachoma and inclusion conjunctivitis. J. gen. Microbiol. 37, 271.CrossRefGoogle ScholarPubMed
Taverne, J., Blyth, W. A. & Reeve, P. (1964 b). Growth in the chick embryo of strains of trachoma and inclusion blennorrhoea virus of differing virulence. J. gen. Microbiol. 37, 277.CrossRefGoogle ScholarPubMed
Thompson, W. R. (1947). Use of moving averages and interpolation to estimate median effective dose. I. Fundamental formulas, estimation of error, and relation to other methods. Bact. Rev. 11, 115.CrossRefGoogle ScholarPubMed