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In vivo R–plasmid transfer in a patient with a mixed infection of shigella dysentery

Published online by Cambridge University Press:  15 May 2009

M. P. Bratoeva  and
J. F. John. Jr
M. P. Bratoeva
Affiliation:
Department of Medicine, Medical University of South Carolina, Charleston, SC
J. F. John. Jr*
Affiliation:
Division of Allergy. Immunology and Infectious Diseases, Robert Wood Johnson Medical School. University of Medicine and Dentistry of New Jersey. New Brunswick, NJ
*
*Correspondence and reprint requests: J. F. John. Jr.. 356 MEB. Robert Wood Johnson Medical School. One Robert Wood Johnson Place. New Brunswick, NJ 08903–0019, USA.
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Transfer of shigella R–plasmids in vivo has seldom been demonstrated. Strains of Shigella dysenteriae type 1 and Shigella flexneri type 5b were isolated from a Bulgarian traveller who visited Vietnam and developed dysentery, which was treated with trimethoprim/sulfamethoxazole (TMP/SMZ) for a short time. Both species of shigellae are unusual in Bulgaria where strains of S. sonnei predominate. Both shigella strains were multiresistant to the same antimicrobial agents. Each strain contained a 48–kilobase plasmid that conferred the entire resistance phenotype to a susceptible Escherichia coli. Restriction endonuclease patterns of plasmid DNA from the respective strains were identical. Transmissible plasmids of the same resistance phenotypes and restriction patterns were isolated from the patient's colonic E. coli. Transconjugants hybridized to a dihydrofolate reductase type I–DNA probe. These studies support the hypothesis that R–plasmid transfer may occur between non-pathogenic, faecal strains and pathogenic shigellae, a process that may have been facilitated by inadequate treatment with TMP/SMZ at the onset of the illness.

Type
Research Article
Information
Epidemiology & Infection , Volume 112 , Issue 2 , April 1994 , pp. 247 - 252
Copyright
Copyright © Cambridge University Press 1994

References

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