Hostname: page-component-77c89778f8-cnmwb Total loading time: 0 Render date: 2024-07-16T14:15:12.493Z Has data issue: false hasContentIssue false
  • English
  • Français

The inactivation of poliovirus in aerosols

Published online by Cambridge University Press:  15 May 2009

J. C. De Jong  and
K. C. Winkler
J. C. De Jong
Affiliation:
Laboratory for Microbiology, State University, Catharijnesingel 59, Utrecht, The Netherlands
K. C. Winkler
Affiliation:
Laboratory for Microbiology, State University, Catharijnesingel 59, Utrecht, The Netherlands
Rights & Permissions [Opens in a new window]

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Poliovirus type 1, strain LSc2ab, was directly sprayed in a static air cabinet at 20° C. and sampled afterwards with impingers. Virus inactivation during spraying was maximal in dry air; during storage maximal decay occurred at moderate humidity.

With regard to the mechanism of the inactivation the following facts have been observed: (1) The RNA in the virus decays simultaneously with the whole virus particle. (2) Oxidation does not play any significant role. (3) Incubation with L-cystine protects the virus against inactivation at 50° C. in aqueous suspension, but not against decay in aerosols.

On the strength of these observations it is concluded that in aerosols denatura-tion of the viral RNA is the cause of the inactivation of poliovirus. The decay in aerosols has some features in common with the thermal inactivation in aqueous suspensions at room temperature.

Type
Research Article
Information
Journal of Hygiene , Volume 66 , Issue 4 , December 1968 , pp. 557 - 565
Copyright
Copyright © Cambridge University Press 1968

References

REFERENCES

Alexander, H. E., Koch, G., Mountain, I. M. & van Damme, O. (1958). Infectivity of ribonucleic acid from poliovirus in human cell monolayers. J. exp. Med. 108, 493.Google Scholar
Buckland, F. E. & Tyrrell, D. A. J. (1962). Loss of infectivity on drying various viruses. Nature, Lond. 195, 1063.CrossRefGoogle ScholarPubMed
Dimmock, N. J. (1967). Differences between the thermal inactivation of picornaviruses at ‘high’ and ‘low’ temperatures. Virology 31, 338.CrossRefGoogle ScholarPubMed
Doorschodt, H. J. (1961). De betekenis van continue cellijnen voor de virologie. Boerhaavecursus p. 20.Google Scholar
Ginoza, W., Hoelle, C. J., Vessey, K. B. & Carmack, C. (1964). Mechanisms of inactivation of single-stranded virus nucleic acids by heat. Nature, Lond. 203, 606.CrossRefGoogle ScholarPubMed
Harper, G. J. (1961). Airborne micro-organisms: survival tests with four viruses. J. Hyg., Camb. 59, 479.Google ScholarPubMed
Harper, G. J. (1963). Some observations on the influence of suspending fluids on the survival of airborne viruses. First Int. Symp. on Aerobiol., Berkeley 1963, pp. 335.Google Scholar
Hemmes, J. H., Winkler, K. C. & Kool, S. M. (1960). Virus survival as a seasonal factor in influenza and poliomyelitis. Nature, Lond. 188, 430.Google Scholar
Hemmes, J. H., Winkler, K. C. & Kool, S. M. (1962). Virus survival as a seasonal factor in influenza and poliomyelitis. Antonie van Leeuwenhoek 28, 221.CrossRefGoogle Scholar
De Jong, J. C. (1967). Ph.D. Thesis, University of Utrecht.Google Scholar
Koch, G. (1960). Influence of assay conditions on infectivity of heated poliovirus. Virology 12, 601.CrossRefGoogle ScholarPubMed
Lund, E. & Lycke, E. (1961). The effect of oxidation and reduction on the infectivity of poliomyelitis virus. Arch. ges. Virusforsch. 11, 100.CrossRefGoogle ScholarPubMed
Pohjanpelto, P. (1958). Stabilization of poliovirus by cystine. Virology 6, 472.CrossRefGoogle ScholarPubMed
Pohjanpelto, P. (1962). Oxidation in thermoinactivation of poliovirus. Virology 16, 92.CrossRefGoogle ScholarPubMed
Rosebury, T. and others (1947). Experimental Air-borne Infection. Baltimore: Williams and Wilkins.Google Scholar
Webb, S. J., Bather, R. & Hodges, R. W. (1963). The effect of relative humidity and inositol on air-borne viruses. Can. J. Microbiol. 9, 87.CrossRefGoogle Scholar
Woese, C. (1960). Thermal inactivation of animal viruses. Ann. N.Y. Acad. Sci. 83, 741.CrossRefGoogle ScholarPubMed