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Investigation into rabbit infusion media for the growth of Mycoplasma gallisepticum antigens for inoculation into rabbits

Published online by Cambridge University Press:  15 May 2009

Janet M. Bradbury  and
F. T. W. Jordan
Janet M. Bradbury
Affiliation:
Poultry Research Section, University of Liverpool Veterinary Field Station ‘Leahurst’, Neston, Wirral, Cheshire
F. T. W. Jordan
Affiliation:
Poultry Research Section, University of Liverpool Veterinary Field Station ‘Leahurst’, Neston, Wirral, Cheshire
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Summary

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Serum cholesterol in rabbits was elevated, by intravenous inoculation of 20 % Tween 80, to amounts thought to have been sufficient to support growth of Mycoplasma gallisepticum when 20 % of such serum was added to rabbit infusion broth.

However, better growth of the organism was obtained with a supplement of 5 % rabbit serum and commercial cholesterol. Investigation showed that normal rabbit serum may be inhibitory, which would explain these findings. The commercial cholesterol was not antigenic.

Type
Research Article
Information
Journal of Hygiene , Volume 69 , Issue 1 , March 1971 , pp. 73 - 82
Copyright
Copyright © Cambridge University Press 1971

References

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