Hostname: page-component-77c89778f8-7drxs Total loading time: 0 Render date: 2024-07-20T23:47:04.182Z Has data issue: false hasContentIssue false
  • English
  • Français

Isolation and characterization of motile Aeromonas from human, food and environmental specimens

Published online by Cambridge University Press:  15 May 2009

Yoshikazu Nishikawa  and
Teruhiko Kishi
Yoshikazu Nishikawa
Affiliation:
Department of Bacteriology, Osaka CityInstitute of Public Health and Environmental Sciences, Tennoji, Osaka 543, Japan.
Teruhiko Kishi
Affiliation:
Department of Bacteriology, Osaka CityInstitute of Public Health and Environmental Sciences, Tennoji, Osaka 543, Japan.
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

From July 1985 to March 1987, the occurrence of motile Aeromonas sp. in stool, food and environmental specimens was investigated to assess their pathogenic significance and to determine sources and routes of infection. A total of 9366 stool specimens were examined; Aeromonas was isolated from 11.1% of diarrhoeal stools and 2.2% of normal stools (P 0.001). Aeromonas counts in food specimens, which included minced beef, pork and chicken, seafood and various vegetables and their products, were unexpectedly high suggesting that infection might be food–borne rather than water–borne. About 70% of the isolates from meat products were A. hydrophila and A. sobria, while A. Caviae was the most common in sea–fish, vegetables and their products. Most A. hydrophila and A. sobria strains produced haemolysin, but haemagglutinin was found more frequently in A. Sobria.

Type
Research Article
Information
Epidemiology & Infection , Volume 101 , Issue 2 , October 1988 , pp. 213 - 223
Copyright
Copyright © Cambridge University Press 1988

References

Agger, W. A., Mccormick, J. D. & Gurwith, M. J. (1985). Clinical and microbiological features of Aeromonas hydrophila-associated diarrhea. Journal of Clinical Microbiology 21, 909–913.CrossRefGoogle ScholarPubMed
Altwegg, M. (1985). Aeromonas caviae: an enteric pathogen? Infection 5, 228–230.Google Scholar
American Public Health Association (1971). Standard Methods for the Examination of Water and Waste Water, 13th ed., pp. 672. New York: American Public Health Association.Google Scholar
Asao, T., Kozaki, S., Kato, K., Kinoshita, Y., Otsu, K., Uemura, T. & Sakaguchi, G. (1986). Purification and characterization of an Aeromonas hydrophila hemolysin. Journal of Clinical Microbiology 24, 228–232.CrossRefGoogle ScholarPubMed
Atkinson, H. M. & Trust, T. J. (1980). Hemagglutination properties and adherence ability of Aeromonas hydrophila. Infection and Immunity 27, 938–946.CrossRefGoogle ScholarPubMed
Burke, V., Cooper, M., Robinson, J., Gracey, M., Lesmana, M., Echeverria, P. & Janda, J. M. (1984a). Hemagglutination patterns of Aeromonas spp. in relation to biotype and source. Journal of Clinical Microbiology 19, 39–43.CrossRefGoogle ScholarPubMed
Burke, V., Robinson, J., Gracey, M., Peterson, P. & Partridge, K. (1984b). Isolation of Aeromonas hydrophila from a metropolitan water supply: seasonal correlation with clinical isolates. Applied and Environmental Microbiology 48, 361–366.CrossRefGoogle ScholarPubMed
Burke, V., Gracey, M., Robinson, J., Peck, D., Beaman, J. & Bundell, C. (1983). The microbiology of childhood gastroenteritis: Aeromonas species and other infective agents. Journal of Infectious Diseases 148, 68–74.CrossRefGoogle ScholarPubMed
Champsaur, H., Andremont, A., Mathieu, D., Rottman, E. & Auzepy, P. (1982). Cholera-like illness due to Aeromonas sobria. Journal of Infectious Diseases 145, 248–254.CrossRefGoogle ScholarPubMed
Chopra, A. K., Houston, C. W., Genaux, C. T.Dixon, J. D. & Kurosky, A. (1986). Evidence for production of an enterotoxin and cholera toxin cross-reactive-factor by Aeromonas hydrophila. Journal of Clinical Microbiology 24, 661–664.CrossRefGoogle ScholarPubMed
Cumberbatch, N., Gurwith, M. J., Langston, C., Sack, R. B. & Brunton, J. L. (1979). Cytotoxic enterotoxin produced by Aeromonas hydrophila: relationship of toxigenic isolates to diarrheal disease. Infection and Immunity 23, 829–837.CrossRefGoogle ScholarPubMed
Daily, O. P., Joseph, S. W., Coolbaugh, J. C., & Walker, R. I., Merrell, B. R., Rollins, D. M., Seidler, R. J., Colwell, R. R. & Lissner, C. R. (1981). Association of Aeromonas sobria with human infection. Journal of Clinical Micorbiology 13, 769–777.CrossRefGoogle ScholarPubMed
Echeverria, P., Blacklow, N. R., Sanford, L. B. & Cukor, G. C. (1981). Travelers' diarrhea among American Peace Corps volunteers in rural Thailand. Journal of Infectious Diseases 143, 767–771.CrossRefGoogle ScholarPubMed
Echeverria, P., Sack, R. B., Blacklow, N. R., Bodhidatta, P., Rowe, B. & Mcfarland, A. (1984). Prophylactic doxycycline for travelers' diarrhea in Thailand. American Journal of Epidemiology 120, 912–921.CrossRefGoogle ScholarPubMed
Evans, D. J., JR., Evans D. G. & DuPont, H. L. (1979). Hemagglutination patterns of enterotoxigenic and enteropathogenic Escherichia coli determined with human, bovine, chicken, and guinea pig erythrocytes in the presence and absence of mannose. Infection and Immunity 23, 336–346.CrossRefGoogle ScholarPubMed
Evans, D. G., Satterwhite, T. K., Evans, D. J. & Dupont, H. L. (1978). Differences in serological responses and excretion patterns of volunteers challenged with enterotoxigenic Escherichia coli with and without the colonization factor antigen. Infection and Immunity 19. 883–888.CrossRefGoogle ScholarPubMed
Figura, N., Marri, L., Verdiani, S., Ceccherini, C. & Barberi, A. (1986). Prevalence, species differentiation, and toxigenicity of Aeromonas strains in cases of childhood gastroenteritis and in controls. Journal of Clinical Microbiology 23, 595–599.CrossRefGoogle ScholarPubMed
Freter, R. (1978). Association of enterotoxigenic bacteria with the mucosa of the small intestine: mechanisms and pathogenic implications. In Cholera and Related Diarrheas, (ed. Ochterlony, O., Holmgren, J., Craig, J., Barua, D.), pp. 155–170. Basel, Switzerland: S. Karger.Google Scholar
Furniss, A. L., Lee, J. V. & Donovan, T. J. (1978). The Vibrios. In Public Health Laboratory Service Monograph Series. London: Her Majesty's Stationery Office.Google Scholar
Gosling, P. J. (1986). Biochemical characteristics, enterotoxigenicity and susceptibility to antimicrobial agents of clinical isolates of Aeromonas species encountered in the western region of Saudi Arabia. Journal of Medical Microbiology 22, 51–55.CrossRefGoogle ScholarPubMed
Gracey, M., Burke, V., Robinson, J., Masters, P. L., Stewart, J. & Pearman, J. (1984). Aeromonas spp. in travellers' diarrhoea. British Medical Journal 289, 658.CrossRefGoogle ScholarPubMed
Gregersen, T. (1978). Rapid method for distinction of gram–negative from gram-positive bacteria. European Journal of Applied Microbiology and Biotechnology 5, 123–127.CrossRefGoogle Scholar
Honda, T., Sato, M., Nishimura, T., Higashitsutsumi, M., Fukai, K. & Miwatani, T. (1985). Demonstration of cholera toxin-related factors in cultures of Aeromonas species by enzyme–linked immunosorbent assay. Infection and Immunity 50, 322–323.CrossRefGoogle ScholarPubMed
Janda, J. M., Bottone, E. J. & Reitano, M. (1983). Aeromonas species in clinical microbiology: significance, epidemiology, and speciation. Diagnostic Microbiology and Infectious Diseases 1, 221–228.CrossRefGoogle ScholarPubMed
Kaper, J., Ramon, J., Seidler, J., Lockman, H. & Colwell, R. R. (1979). Medium for the presumptive identification of Aeromonas hydrophila and Enterobacteriaceae. Applied and Environmental Microbiology 38, 1023–1026.CrossRefGoogle ScholarPubMed
Kindschuh, M., Pickering, L. K., Cleary, T. G. & R.–Palacios, G. (1987). Clinical and biochemical significance of toxin production by Aeromonas hydrophila. Journal of Clinical Microbiology 25, 916–921.CrossRefGoogle ScholarPubMed
Lawson, M. A., Burke, V. & Chang, B. J. (1985). Invasion of HEp–2 cells by fecal isolates of Aeromonas hydrophila. Infection and Immunity 47, 680–683.CrossRefGoogle ScholarPubMed
le chevallier, M. W., Evans, T. M., Seidler, R. J., Daily, O. P., Merrell, B. R., Rollins, D. M. & Joseph, S. W. (1982). Aeromonas sobria in chlorinated drinking water supplies. Microbial Ecology 8, 325–333.CrossRefGoogle Scholar
Ljungh, Å., Popoff, M. & Wadströ, T. (1977). Aeromanas hydrophila in acute diarrheal disease: detection of enterotoxin and biotyping of strains. Journal of Clinical Microbiology 6. 96–100.CrossRefGoogle Scholar
Millership, S. E. & Chattopadhyay, B. (1984). Methods for the isolation of Aeromonas hydrophila and Plesiomonas shigelloides from faeces. Journal of Hygiene 92, 145–152.CrossRefGoogle ScholarPubMed
Millership, S. E., Curnow, S. R. & Chattopadhyay, B. (1983). Faecal carriage rate of Aeromonas hydrophila. Journal of Clinical Pathology 36, 920–923.CrossRefGoogle ScholarPubMed
Morgan, D. R., Johnson, P. C., DuPont, H. L., Satterwhite, T. K. & Wood, L. V. (1985). Lack of correlation between known virulence properties of Aeromonas hydrophila and enteropathogenicity for humans. Infection and Immunity 50, 62–65.PCrossRefGoogle ScholarPubMed
Nishikawa, Y. & Kishi, T. (1987). A modification of bile salts brilliant green agar for isolation of motile Aeromonas from foods and environmental specimens. Epidemiology and Infection 98, 331–336.CrossRefGoogle ScholarPubMed
Pitarangsi, C., Echeverria, P., Whitmire, R., Tirapat, C., Formal, S., Dammin, G. J. & Tingtalapong, M. (1982). Enteropathogenicity of Aeromonas hydrophila and Plesiomonas shigelloides: prevalence among individuals with and without diarrhea in Thailand. Infection and Immunity 35, 666–673.CrossRefGoogle ScholarPubMed
Popoff, M. (1984). Aeromonas. In Bergey's Manual of Systematic Bacteriology, Vol. 1 (ed. Krieg, N. R.Holt, J. G.), pp. 545–548. Baltimore: Williams and Wilkins.Google Scholar
Rahman, A. F. M.S., & Willoughby, J. M. T. (1980). Dysentery–like syndrome associated with Aeromonas hydrophila. British Medical Journal 281, 976.CrossRefGoogle ScholarPubMed
Satterwhite, T. K., Evans, D. G., Dupont, H. L. & Evans, D. J. (1978). Role of Escherichia coli colonisation factor antigen in acute diarrhea. Lancet ii, 181–184.CrossRefGoogle Scholar
Stelma, G. N. Jr., Johnson, C. H. & Spaulding, P. (1986). Evidence for the direct involvement of hemolysin in Aeromonas hydrophila enteropathogenicity. Current Microbiology 14, 71–77.CrossRefGoogle Scholar
Turnbull, P. C. B., Lee, J. V., Miliotis, M. D., Walle, S. V. D., Koornhof, H. J., Jeffery, L. & Bryant, T. N. (1984). Enterotoxin production in relation to taxonomic grouping and source of isolation of Aeromonas species., Journal of Clinical Microbiology 19, 175–180.CrossRefGoogle ScholarPubMed
Watson, I. M., Robinson, J. O., Burke, V. & Gracey, M. (1985). Invasiveness of Aeromonas spp. in relation to biotype, virulence factors, and clinical features. Journal of Clinical Microbiology 22, 48–51.CrossRefGoogle ScholarPubMed