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Isolation of enterotoxigenic Escherichia coli from British troops in Saudi Arabia

Published online by Cambridge University Press:  15 May 2009

G. A. Willshaw ,
T. Cheasty ,
B. Rowe ,
H. R. Smith ,
D. N. Faithfull-Davies  and
T. G. J. Brooks
G. A. Willshaw
Affiliation:
Laboratory of Enteric Pathogens, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT, UK
T. Cheasty
Affiliation:
Laboratory of Enteric Pathogens, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT, UK
B. Rowe
Affiliation:
Laboratory of Enteric Pathogens, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT, UK
H. R. Smith
Affiliation:
Laboratory of Enteric Pathogens, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT, UK
D. N. Faithfull-Davies
Affiliation:
Leishman Laboratory, Cambridge Military Hospital, Aldershot, Hants GU11 2AN, UK
T. G. J. Brooks
Affiliation:
CBDE, Porton Down, Salisbury SP4 0JQ, UK
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Specimens from 181 patients with diarrhoea were examined by a Military General Hospital in a 3-month period during deployment of troops to Saudi Arabia in 1990/1. DNA probes for heat labile (LT) and heat stable (ST) enterotoxin genes identified enterotoxigenic Escherichia coli (ETEC) in 47 of the specimens (26%) and 49 ETEC strains were isolated. The majority (55%) belonged to a novel ETEC serotype having the O-antigen 159 and a flagellar antigen designated as a provisional new type. They produced ST and the coli surface associated antigen (CS)6. Strains of serotype O6:H16 represented 22% of the ETEC examined. They produced ST, LT and CSS together with either CS1 or CS2. The remaining ETEC belonged to seven O:H serotypes. Overall, ST was the only enterotoxin gene identified in 73% of the ETEC and 67% of the strains expressed CS6 in the absence of other colonization antigens. Resistance to three or more antibiotics was observed in 53% of the ETEC, including most of the O159 strains.

Type
Research Article
Information
Epidemiology & Infection , Volume 115 , Issue 3 , December 1995 , pp. 455 - 463
Copyright
Copyright © Cambridge University Press 1995

References

1.Black, RE. Pathogens that cause travelers' diarrhea in Latin America and Africa. Rev Infect Dis 1986; 8 (Suppl. 2): S131–5.CrossRefGoogle ScholarPubMed
2.Echeverria, P, Blacklow, NR, Sanford, LB, Cukor, GG. Travelers' diarrhea among American Peace Corps Volunteers in rural Thailand. J Infect Dis 1981; 143: 767–71.CrossRefGoogle ScholarPubMed
3.DuPont, HL, Galindo, E, Evans, DG, Cabada, FJ, Sullivan, P, Evans, DJ Jr. Prevention of travelers' diarrhea with trimethoprim-sulfamethoxazole and trimethoprim alone. Gastroenterol 1983; 84: 75–80.CrossRefGoogle ScholarPubMed
4.Sack, DA, Kaminsky, DC, Sack, RB et al. Enterotoxigenic Escherichia coli diarrhea of travelers: a prospective study of American Peace Corps volunteers. Johns Hopkins Med J 1977; 141: 63–70.Google ScholarPubMed
5.Black, RE, Merson, MH, Huq, I, Abdul Alim, ARM, Yunus, M. Incidence and severity of rotavirus and Escherichia coli diarrhoea in rural Bangladesh. Lancet 1981; i: 141–3.CrossRefGoogle Scholar
6.Rowe, B, Gross, R, Takeda, Y. Serotyping of enterotoxigenic Escherichia coli isolated from diarrhoeal travellers from various Asian countries. FEMS Microbiol Lett 1983; 20: 187–9.CrossRefGoogle Scholar
7.McConnell, MM. Newly characterized putative colonization factors of human enterotoxigenic Escherichia coli. In: Wadstrom, T.Makela, PH, Svennerholm, A-M, Wolf-Watz, H. eds. Molecular pathogenesis of gastrointestinal infections. New York: Plenum Press. 1991: 79–85.CrossRefGoogle Scholar
8.Svennerholm, A-M, Lopez-Vidal, Y, Holmgren, J, McConnell, MM, Rowe, B. Role of PCF8775 antigen and its coli surface components for colonization, disease, and protective immunogenicity of enterotoxigenic Escherichia coli in rabbits. Infect Immun 1988; 56: 523–8.CrossRefGoogle ScholarPubMed
9.Kauffmann, F. Serological diagnosis of Salmonella species. Copenhagen: Munksgaard E. 1972.Google Scholar
10.Anderson, ES, Ward, LR, de Saxe, MJ, de Sa, JDH. Bacteriophage-typing designation of Salmonella typhimurium. J Hyg 1959; 57: 346–59.Google Scholar
11.Cowan, ST. Cowan and Steel's manual for the identification of medical bacteria. 2nd edn.Cambridge: Cambridge University Press. 1974.Google Scholar
12.Maniatis, T, Fritsch, EF, Sambrook, J. Molecular cloning: a laboratory manual, Cold Spring Harbor, NY, Cold Spring Harbor Laboratory, 1982.Google Scholar
13.McConnell, MM, Thomas, LV, Willshaw, GA, Smith, HR, Rowe, B. Genetic control and properties of coli surface antigens of colonization factor antigen IV (PCF 8775) of enterotoxigenic Escherichia coli. Infect Immun 1988; 56: 1974–80.CrossRefGoogle Scholar
14.Scotland, SM, Willshaw, GA, Said, B, Smith, HR, Rowe, B. Identification of Escherichia coli that produces heat-stable enterotoxin STa by a commercially available enzyme-linked immunoassay and comparison of the assay with infant mouse and DNA probe tests. J Clin Microbiol 1989; 27: 1697–9.CrossRefGoogle ScholarPubMed
15.Gross, RJ, Rowe, B. Serotyping of Escherichia coli. In: The virulence of Escherichia coli. Reviews and Methods. Sussman, M. ed. London: Academic Press. 1985: 345–63.Google Scholar
16.Cravioto, A, Scotland, SM, Rowe, B. Hemagluttination activity and colonization factor antigens I and II in enterotoxigenic and non-enterotoxigenic strains of Escherichia coli isolated from humans. Infect Immun 1982; 36: 189–97.CrossRefGoogle Scholar
17.McConnell, MM, Rowe, B. Prevalence of the putative colonization factors CFA/III and PCFO159:H4 in enterotoxigenic Escherichia coli. J Infect Dis 1989; 159: 582–6.CrossRefGoogle ScholarPubMed
18.Anderson, ES, Threlfall, EJ. The characterisation of plasmids in the enterobacteria. J Hyg 1974; 72: 471–87.CrossRefGoogle ScholarPubMed
19.Birnboim, HC, Doly, J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 1979; 7: 1513–23.CrossRefGoogle ScholarPubMed
20.Rowe, B, Taylor, J, Bettelheim, KA. An investigation of travellers' diarrhoea. Lancet 1970; i: 1–5.CrossRefGoogle Scholar
21.Hyams, KC, Bourgeois, AL, Merrell, BR et al. Diarrheal disease during Operation Desert Shield. N Engl J Med 1991; 325: 1423–8.CrossRefGoogle ScholarPubMed
22.Sethi, SK, Khuffash, FA, Al-Nakib, W. Microbial etiology of acute gastroenteritis in hospitalized children in Kuwait. Pediatr Infect Dis J 1989; 8: 593–7.CrossRefGoogle ScholarPubMed
23.Wolf, MV, Taylor, DN, Boedeker, EC et al. Characterization of enterotoxigenic Escherichia coli isolated from U.S. troops deployed to the Middle East. J Clin Microbiol 1993; 31: 851–6.CrossRefGoogle ScholarPubMed
24.McConnell, MM, Hibberd, ML, Penny, ME, Scotland, SM, Cheasty, T, Rowe, B. Surveys of human enterotoxigenic Escherichia coli from three different geographical areas for possible colonization factors. Epidemiol Infect 1991; 106: 477–84.CrossRefGoogle ScholarPubMed
25.Gross, RJ, Rowe, B, Henderson, A, Byatt, ME, Maclaurin, JC. A new Escherichia coli O-group, O159, associated with outbreaks of enteritis in infants. Scand J Infect Dis 1976; 8: 195–8.CrossRefGoogle ScholarPubMed
26.Kudoh, Y, Zen-Yoji, H, Matsushita, S, Sakai, S, Maruyama, T. Outbreaks of acute enteritis due to heat stable enterotoxin-producing strains of Escherichia coli. Microbiol Immunol 1977; 21: 175–8.CrossRefGoogle ScholarPubMed
27.Tacket, CO, Maneval, DR, Levine, MM. Purification, morphology and genetics of a new fimbrial putative colonization factor of enterotoxigenic Escherichia coli O159:H4. Infect Immun 1987; 55: 1063–9.CrossRefGoogle ScholarPubMed
28.Thomas, LV, Rowe, B. The occurrence of colonisation factors (CFA/I, CFA/II and E8775) in enterotoxigenic Escherichia coli from various countries in South East Asia. Med Microbiol Immunol 1982; 171: 85–90.CrossRefGoogle ScholarPubMed