Hostname: page-component-848d4c4894-m9kch Total loading time: 0 Render date: 2024-06-01T07:17:53.909Z Has data issue: false hasContentIssue false
  • English
  • Français

Methicillin-resistant Staphylococcus aureus from China characterized by digestion of total DNA with restriction enzymes

Published online by Cambridge University Press:  15 May 2009

L. M. C. Hall ,
J. Z. Jordens  and
F. Wang
L. M. C. Hall
Affiliation:
Department of Medical Microbiology, London Hospital Medical College, Turner Street, London E1 2AD
J. Z. Jordens
Affiliation:
Department of Medical Microbiology, London Hospital Medical College, Turner Street, London E1 2AD
F. Wang
Affiliation:
Institute of Antibiotics, Hua Shan Hospital, Shanghai 200040, China
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

A series of clinical isolates of methicillin resistant Staphylococcus aureus (MRSA) from two hospitals in China was examined. Fragment patterns obtained by digestion of total cellular DNA with restriction enzymes were used to characterize the isolates, in combination with phage-typing, antibiotic resistance profile, and plasmid profile. Digestion of total cellular DNA with restriction enzymes was most useful in discriminating between isolates and yielded additional information on the relatedness of non-identical isolates. In one hospital a single strain, resistant to a large number of antibiotics, had apparently become endemic. In the second hospital a number of distinct but related strains were present. The isolates were also related but not identical to the strain of MRSA endemic at the London Hospital.

Type
Special Article
Information
Epidemiology & Infection , Volume 103 , Issue 1 , August 1989 , pp. 183 - 192
Copyright
Copyright © Cambridge University Press 1989

References

REFERENCES

1.Parker, MT. The significance of phage-typing patterns in Staphylococcus aureus. In: Easmon, CSF, Adlam, C, eds. Staphylococci and staphyloeoccal infections. Academic Press, 1983: 33–62.Google Scholar
2.Gaston, MA, Duff, PS, Naidoo, J, Ellis, K, Roberts, JIS, Richardson, JF, Marples, RR, Cooke, EMEvaluation of electrophoretie methods for typing methicillin-resistant Staphylococcus aureus. J Med Micro 1988; 26, 189–97.CrossRefGoogle ScholarPubMed
3.Jordens, JZ, Hall, LMC. Characterisation of methicillin-resistant Staphylococcus oureus. J Med Micro 1988; 27: 117–23.CrossRefGoogle Scholar
4.Bennett, PM, Heritage, J, Hawkey, PM. An ultra-rapid method for the study of antibiotic plasmids. J Antimicrobial Chemotherapy 1986; 18: 421–4.CrossRefGoogle Scholar
5.Dice, LR. Measures of the amount of ecological association between species. Ecology 1945; 26: 297–302.CrossRefGoogle Scholar
6.Townsend, DE, Ashdown, N, Bolton, S, Bradley, J, Duckworth, G, Moorhouse, EC, Grubb, WB.The international spread of methicillin-resistant Stophylococcus aureus. J Hosp Infect 1987; 9: 60–71.CrossRefGoogle Scholar
7.Marples, RR, Richardson, JF, de Saxe, MJ. Bacteriological characters of strains of Staphylococcus aureus submitted to a reference laboratory related to methicillin resistance. J Hyg 1986; 96: 217—23.CrossRefGoogle ScholarPubMed