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A modification of bile salts brilliant green agar for isolation of motile Aeromonas from foods and environmental specimens

Published online by Cambridge University Press:  19 October 2009

Yoshikazu Nishikawa
Affiliation:
Department of Bacteriology, Municipal Institute of Public Health and Environment Science, Tennoji, Osaka 543, Japan
Teruhiko Kishi
Affiliation:
Department of Bacteriology, Municipal Institute of Public Health and Environment Science, Tennoji, Osaka 543, Japan
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Aeromonas has recently been recognized as an important enteropathogen. In faecal specimens the organism is readily identified and differentiated from coliforms by the positive oxidase reaction. However, few media have proved useful for foods and environmental specimens. Bile salts brilliant green starch agar (BBGS) was studied for this purpose by testing artificially contaminated foods as well as natural potential sources. The results indicate the suitability of the medium for the isolation of Aeromonas from foods and from environmental sources.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1987

References

REFERENCES

Burke, V., Robinston, J., Gracey, M., Peterson, D. & Partridge, K. (1984). Isolation of Aeromonas hydrophila from a metropolitan water supply: seasonal correlation with clinical isolates. Applied and Environmental Microbiology 48, 361366.CrossRefGoogle ScholarPubMed
Gregersen, T. (1978). Rapid method for distinction of gram-negative from gram-positive bacteria. European Journal of Applied Microbiology and Biotechnology 5, 123127.CrossRefGoogle Scholar
Holmberg, S. E. & Farmer, J. J. III (1984). Aeromonas hydrophila and Plesiomonas shigelloides as causes of intestinal infections. Reviews of Infectious Diseases 6, 633639.CrossRefGoogle ScholarPubMed
Kaper, J., Seidler, R. J., Lockman, H. & Colwell, R. R. (1979). A medium for the presumptive identification of Aeromonas hydrophila and Enterobacteriaceae. Applied and Environmental Microbiology 38, 10231026.CrossRefGoogle ScholarPubMed
Millership, S. E. & Chattopadhyay, B. (1984). Methods for the isolation of Aeromonas hydrophila and Plesiomonas shigelloides from faeces. Journal of Hygiene 92, 145152.Google Scholar
Motyl, M. R., McKinley, G. & Janda, J. M. (1985). In vitro susceptibilities of Aeromonas hydrophila, Aeromonas sobria, and Aeromonas caviae to 22 antimicrobial agents. Antimicrobial Agents and Chemotherapy 28, 151153.Google Scholar
Palumbo, S. A., Maxino, F., Williams, A. C., Buchanan, R. L. & Thayer, D. W. (1985). Starchampicillin agar for the quantitative detection of Aeromonas hydrophila. Applied and Environmental Microbiology 38, 10231026.Google Scholar
Popoff, M. (1984). Aeromonas. In Bergey's Manual of Systematic Bacteriology, vol. 1 (ed. Krieg, N. R. and Holt, J. G.), pp. 545548. Baltimore: Williams and Wilkins.Google Scholar
Rahim, Z., Sanyal, S. C., Aziz, K. M. S., Huq, M. I. & Chowdhury, A. A. (1984). Isolation of enterotoxigenic, hemolytic, and antibiotic-resistant Aeromonas hydrophila strains from infected fish in Bangladesh. Applied and Environmental Microbiology 48, 865867.CrossRefGoogle ScholarPubMed
Shubert, R. H. W. (1977). Ueber den Nachweis von Plesiomonas shigelloides Habs und Schubert, 1962, und ein Elektivmedium, den Inositol-Brillant grün-Gallesalz-Agar. Ernst-Rodenwaldt Archiv 4, 97103.Google Scholar
Shread, P., Donovan, T. J. & Lee, J. V. (1981). A survey of the incidence of Aeromonas in human faeces. Society for General Microbiology Quarterly 8, 184.Google Scholar
Smith, H. L. Jr & Goodner, K. (1958). Detection of bacterial gelatinases by gelatin-agar plate methods. Journal of Bacteriology 76, 662665.Google Scholar
von Graevenitz, A. & Zinterhofer, L. (1970). The detection of Aeromonas hydrophila in stool specimens. Health Laboratory Science 7, 124127.Google ScholarPubMed