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A novel relationship between O-antigen variation, matrix formation, and invasiveness of Salmonella enteritidis

Published online by Cambridge University Press:  15 May 2009

J. Guard-Petter ,
L. H. Keller ,
M. Mahbubur Rahman ,
R. W. Carlson  and
S. Silvers
J. Guard-Petter
Affiliation:
USDA/ARS, Southeast Poultry Research Laboratory, 934 College Station Road, Athens, GA 30605
L. H. Keller
Affiliation:
University of Pennsylvania, New Bolton Center, Kennett Square, PA
M. Mahbubur Rahman
Affiliation:
University of Georgia, Complex Carbohydrate Research Center, Athens, GA
R. W. Carlson
Affiliation:
University of Georgia, Complex Carbohydrate Research Center, Athens, GA
S. Silvers
Affiliation:
USDA/ARS, Russell Research Center, Athens, GA
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Salmonella enterica Enteritidis in chickens serves as a reservoir for salmonellosis in humans and the structure of its lipopolysaccharide (LPS) has been used to assess invasiveness. Culture from chick spleens generated colonies with an unusual wrinkled morphology, and it is designated the lacy phenotype. To characterize the nature of the morphological change, three isogenic variants were compared. Only the lacy phenotype produced a temperature-dependent cell surface matrix composed of several proteins in association with LPS high molecular weight O-antigen.

Flagellin and a 35 kDa protein were identified as specific proteinaceous components of matrix. Both proteins cross-reacted with a monoclonal antibody previously determined to specifically detect the g-epitope of the Enteritidis monophasic flagella (H-antigen). These results suggest that O-antigen in association with protein contributes to cross-reactivity between molecules. The lacy phenotype was more organ invasive in 5-day-old chicks than isogenic variants producing low molecular weight O-antigen. However, it was no more efficient at contaminating eggs after oral inoculation of hens than a variant that completely lacked O-antigen, thus the lacy phenotype is classified as an intermediately invasive organism. The distinctive colonial phenotype of SE6-E21lacy was used to investigate environmental factors that decreased O/C ratios and contributed to attenuation. In so doing, it was found that growth in complement at 46°C caused matrix producing cells to hyperflagellate and migrate across agar surfaces. These results suggest that the structure of O-antigen might influence the secretion and/or the function of Enteritidis cell-surface proteins. The data also reveal a greater heterogeneity than has been assumed in the phenotype, and possibly the infectious behaviour, of Enteritidis.

Type
Research Article
Information
Epidemiology & Infection , Volume 117 , Issue 2 , October 1996 , pp. 219 - 231
Copyright
Copyright © Cambridge University Press 1996

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