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Observations on the viability of Trichomonas foetus during the process of freezing to –79° C. and thawing in the presence of glycerol
Published online by Cambridge University Press: 15 May 2009
Extract
1. It has been confirmed that Trichomonas foetus fails to survive freezing and thawing in the presence of 10 % glycerol in egg-yolk-citrate diluent. Under the same conditions adequate sperm survival was demonstrated.
2. Trichomonads were particularly sensitive to the toxic effects of glycerol when suspended in egg-yolk citrate.
3. Trichomonads will survive freezing and thawing when suspended in other diluents such as egg-yolk phosphate or milk.
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- Copyright © Cambridge University Press 1956
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