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Plasmid analysis as an epidemiological tool in neurosurgical infections with coagulase-negative staphylococci

Published online by Cambridge University Press:  19 October 2009

Åke Örtqvist ,
Ulrika Ransjö  and
Bengt Wretlind
Åke Örtqvist
Affiliation:
Department of Clinical Microbiology, Karolinska sjukhuset, Stockholm, Sweden
Ulrika Ransjö
Affiliation:
Department of Clinical Microbiology, Karolinska sjukhuset, Stockholm, Sweden
Bengt Wretlind
Affiliation:
Department of Clinical Microbiology, Karolinska sjukhuset, Stockholm, Sweden
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Summary

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Coagulase-negative staphylococci isolated from blood or spinal fluid during a period of 1 year in a department of neurosurgery, were analysed by biotyping, antibiotic resistance pattern and plasmid profiles. Altogether 41 isolates from 19 patients were studied. About 90% of the isolates were Staphylococcus epidermidis. The antibiotic resistance pattern seemed to be closely related to antibiotic usage in the unit. Most common was resistance to penicillin (63%), trimethoprimsulphamethoxazole (49%) and cloxacillin (39%) while resistance to gentamicin was seen in only one strain.

In several cases species and antibiograms were identical in isolates from different patients. Plasmid pattern analysis could then be used for identification of different strains. In one instance, plasmid pattern and restriction enzyme analysis confirmed that two patients probably were infected by the same strain.

Type
Research Article
Information
Epidemiology & Infection , Volume 98 , Issue 3 , June 1987 , pp. 231 - 239
Copyright
Copyright © Cambridge University Press 1987

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