Hostname: page-component-848d4c4894-pjpqr Total loading time: 0 Render date: 2024-06-27T21:17:07.170Z Has data issue: false hasContentIssue false
  • English
  • Français

The platelet aggregation test in group B arbovirus infections

Published online by Cambridge University Press:  15 May 2009

K. Penttinen ,
P. Saikku ,
G. Myllylä ,
M. Brummer-Korvenkontio  and
N. Oker-Blom
K. Penttinen
Affiliation:
Department of Virology, University of Helsinki, Helsinki 29, Finland and The Finnish Red Cross Blood Transfusion Service, Helsinki, Finland
P. Saikku
Affiliation:
Department of Virology, University of Helsinki, Helsinki 29, Finland and The Finnish Red Cross Blood Transfusion Service, Helsinki, Finland
G. Myllylä
Affiliation:
Department of Virology, University of Helsinki, Helsinki 29, Finland and The Finnish Red Cross Blood Transfusion Service, Helsinki, Finland
M. Brummer-Korvenkontio
Affiliation:
Department of Virology, University of Helsinki, Helsinki 29, Finland and The Finnish Red Cross Blood Transfusion Service, Helsinki, Finland
N. Oker-Blom
Affiliation:
Department of Virology, University of Helsinki, Helsinki 29, Finland and The Finnish Red Cross Blood Transfusion Service, Helsinki, Finland
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

A new serological method, the platelet aggregation (PA) test, was used to study Group B arbovirus infections, which are often associated with thrombocytopenia. The method is based on the interaction of antigen-antibody complexes with platelets. The PA test was nearly as good as the haemagglutination-inhibition (HI) test for epidemiological studies of tick-borne encephalitis (16 out of 18 HI positive sera) and was superior to the complement-fixation test which was positive in 10 out of 18 HI positive sera. The PA test could be used in the serological diagnosis of Group B arbovirus infection. The titres with the PA test were of the same magnitude as the HI titres, and about ten times higher than the CF titres. Two pairs of dengue haemorrhagic fever sera showed high initial PA titres. The role of immune complexes in the pathogenesis of haemorrhagic fevers is discussed.

Type
Research Article
Information
Journal of Hygiene , Volume 68 , Issue 1 , March 1970 , pp. 71 - 76
Copyright
Copyright © Cambridge University Press 1970

References

Catalogue of Arthropod-Borne Viruses of the World (1967). (Compiled by Taylor, Richard M..) Public Health Service Publication, no. 1760. U.S. Government Printing Office, Washington, D.C.Google Scholar
Clarke, D. H. & Casals, J. (1958). Techniques for hemagglutination and hemagglutination-inhibition with arthropodborne viruses. American Journal of Tropical Medicine and Hygiene 7, 561.CrossRefGoogle Scholar
Clarke, D. H. & Casals, J. (1965). Arboviruses; Group B. In Viral and Rickettsial Infections of Man, 4th ed. pp. 606–58. Ed. Horsfall, F. L. and Tamm, I.. London: Pitman Medical.Google Scholar
Halonen, P. E., Stewart, J. A. & Hall, A. D. (1967). Rubella hemagglutinin prepared in serum free suspension culture of BHK-21 cells. Annales medicinae experimentalis et biologiae Fenniae 45, 182.Google ScholarPubMed
Halstead, S. B. (1968). Consideration of possible immunologic mechanisms in dengue hemorrhagic fever (Dengue hypersensitive disease) (DHD). Abstracts of the Eighth International Congresses on Tropical Medicine and Malaria, Teheran, 1064.Google Scholar
Lennette, E. H. (1964). In Diagnostic Procedures for Viral and Rickettsial Infections, 3rd ed. p. 57, Ed. Lennette, E. H. and Nathalie, J. Smith, New York: American Public Health Association.Google Scholar
Myllylä, G., Vaheri, A., Vesikari, T. & Penttinen, K. (1969). Interaction between human blood platelets, viruses and antibodies. IV; Post-rubella thrombo-cytopenic purpura and platelet aggregation by rubella antigen-antibody interaction. Clinical and Experimental Immunology 4, 323.Google Scholar
Penttinen, K. & Myllylä, G. (1968). Interaction of human blood platelets, viruses and antibodies. I. Platelet aggregation test with microequipment. Annales medicinae experimentalis et biologiae Fenniae 46, 188.Google ScholarPubMed
Penttinen, K., Myllylä, G., Mäkelä, O. & Vaheri, A. (1969). Soluble antigen-antibody complexes and platelet aggregation. Acta pathologica et microbiologica scandinavica 77, 309.CrossRefGoogle ScholarPubMed
Salminen, A. (1962). A method for production of arthropod-borne viral hemagglutinins in tissue culture. Annales medicinae experimentalis et biologiae Fenniae 40, 174.Google ScholarPubMed