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Rabbit nasopharyngeal colonization by Bordetella pertussis: the effects of immunization on clearance and on serum and nasal antibody levels

Published online by Cambridge University Press:  25 March 2010

L. A. E. Ashworth ,
R. B. Fitzgeorge ,
L. I. Irons ,
C. P. Morgan  and
A. Robinson
L. A. E. Ashworth
Affiliation:
Pathogenic Microbes Research Laboratory, PHLS Centre for Applied Microbiology and Research, Porton Down, Salisbury, Wiltshire SP4 OJG, England
R. B. Fitzgeorge
Affiliation:
Pathogenic Microbes Research Laboratory, PHLS Centre for Applied Microbiology and Research, Porton Down, Salisbury, Wiltshire SP4 OJG, England
L. I. Irons
Affiliation:
Pathogenic Microbes Research Laboratory, PHLS Centre for Applied Microbiology and Research, Porton Down, Salisbury, Wiltshire SP4 OJG, England
C. P. Morgan
Affiliation:
Pathogenic Microbes Research Laboratory, PHLS Centre for Applied Microbiology and Research, Porton Down, Salisbury, Wiltshire SP4 OJG, England
A. Robinson
Affiliation:
Pathogenic Microbes Research Laboratory, PHLS Centre for Applied Microbiology and Research, Porton Down, Salisbury, Wiltshire SP4 OJG, England
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Two Bordetella pertussis antigen preparations, outer membrane protein (OMP) and filamentous haemagglutinin (FHA), and a standard vaccine were used to immunize rabbits, and the effects on nasopharyngeal colonization by the organism were determined. Antibodies were measured in serum and in nasal washes by ELISA before and after challenge of the rabbits with 106 bacteria of strain M2. Recoveries of B. pertussis in nasal washes were used to assess colonization, which in controls persisted for at least 65 days. Some rabbits of all the immunized groups showed enhanced clearance, but there was no correlation between the elimination of B. pertussis and serum antibodies to OMP, FHA, lipopolysaccharide, lymphocytosis-promoting factor or agglutinogen 3. In contrast, nasal IgA antibody to FHA showed significant inverse correlation with bacterial persistence. Such antibody was induced by the OMP preparation as well as by FHA, but to different extents depending on the immunization schedule and adjuvant used.

Type
Research Article
Information
Journal of Hygiene , Volume 88 , Issue 3 , June 1982 , pp. 475 - 486
Copyright
Copyright © Cambridge University Press 1982

References

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