Hostname: page-component-848d4c4894-pftt2 Total loading time: 0 Render date: 2024-06-09T05:02:12.781Z Has data issue: false hasContentIssue false
  • English
  • Français

Salmonellas in Danish pigs: a comparison of three isolation methods

Published online by Cambridge University Press:  19 October 2009

Niels Skovgaard ,
Sven Gade Christensen  and
A. W. Gulistani
Niels Skovgaard
Affiliation:
Institute of Hygiene and Microbiology, Royal Veterinary and Agricultural University, Copenhagen, Denmark
Sven Gade Christensen
Affiliation:
Institute of Hygiene and Microbiology, Royal Veterinary and Agricultural University, Copenhagen, Denmark
A. W. Gulistani
Affiliation:
Faculty of Veterinary Science, University of Kabul, Kabul, Afghanistan
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Caecal samples from 350 Danish bacon pigs were investigated for salmonella using three methods of isolation. (1) Direct inoculation of 1 g of faeces into 10 ml of Muller–Kaufmann medium (MK medium) with addition of 0·3% Teepol 610 and subculture on Brilliant Green lactose sucrose phenol-red agar (BLSF agar) with 0·3% Teepol G10. (2) Pre-enrichment of 5 g of faeces into buffered peptone water with addition of 1% Teepol 610 followed by enrichment of 1 ml in 10 ml MK medium with 1% Teepol 610 and subculture on BLSF agar with 0·3% Teepol. (3) Incubation of 0·1 ml of the pre-enrichment (2) into 10 ml Rappaport-Vassiliadis medium (RV 10 medium) incubated at 43°C, subculture on BLSF agar.

The MK media with and without pre-enrichment yielded higher findings than the RV 10 media. In total, 28 (8%) of the pigs were found positive, representing 11 (7·4%) of a total of 142 herds investigated. Lymph glands were collected at a later date from six of the positive herds. Five of the herds were found positive.

The number of salmonellas in the glands was low, probably less than ten per gram.

Type
Research Article
Information
Journal of Hygiene , Volume 95 , Issue 1 , August 1985 , pp. 69 - 75
Copyright
Copyright © Cambridge University Press 1985

References

REFERENCES

Andersen, F. M. (1957). Experiments with union-active or non-ionised, surface-active synthetic compounds for the inhibition of the swarming of proteus bacteria. Nordisk Veterinärmedicin 9, 543–559.Google Scholar
Anon. (1981 a). SVS-Information (State Veterinary Serum Laboratory), July 1981.Google Scholar
Anon. (1981 b). International Standard ISO 6579–1981. Microbiology – general guidance on methods for the detection of salmonella. International Organisation for Standardisation, Geneva.Google Scholar
Anon. (1983, 1984). VD sygdomsregistrering (Directorate for Veterinary Services: registration of diseases), juli–september 1983, september–december 1983, january–marts 1984, april–juni 1984.Google Scholar
Chung, K. C. & Goepfert, J. M. (1970). Growth of salmonella at low pH. Journal of Food Science 35, 326–328.CrossRefGoogle Scholar
Corlet, A. & Brown, M. H. (1980). pH and acidity. In Microbial Ecology of Foods, vol. 1 (ed. in chief Silliker, J. H.), pp. 92–111. London: Academic Press.Google Scholar
Døll, W. (1956). Hemmung des Schwärmens von Proteus-Bakterien durch oberflächenaktive Substanzen (Prill und Rei). Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. I. Abteilung: Originale 166, 43–47.Google Scholar
Haddock, R. L. (1970). Efficacy of examining rectal swabs to detect swine salmonella carriers. American Journal of Veterinary Research 31, 1509–1512.Google ScholarPubMed
Harvey, R. W. S., Price, T. H. & Xirouchaki, E. (1979). Comparison of selenite F, Muller–Kaufmann tetrathionate and Rappaport's medium for the isolation of salmonellas from sewage-polluted natural water using a pre-enrichment technique. Journal of Hygiene 83, 451–460.CrossRefGoogle Scholar
Harvey, R. W. & Price, T. H. (1983). A comparison of two modifications of Rappaport's enrichment medium (R25 and RV) for the isolation of salmonellas from sewage polluted natural water. Journal of Hygiene 91, 451–458.CrossRefGoogle ScholarPubMed
Jameson, J. E. & Emberley, N. W. (1956). A substitute for bile salts in culture media. Journal of General Microbiology 15, 198.CrossRefGoogle Scholar
Kohler, W., Ghatek, S. N., Rische, H. & Ziesche, K. (1961). Arylsulfatasebildung durch Mikroorganismen, besonders durch Salmonella paralyphi B. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. I. Abteilung: Originale 201, 482–488.Google Scholar
Kremastinou, J., Thermoyanni, St., Kalapothaki, V., Mavromatti, Ch., Vassiliadis, P. & Séré, Ch. (1982). Improved isolation of salmonella from meat products with the use of Rappaporfc–Vassiliadis enrichment medium. Archives de l'Institut Pasteur Hellénique XXVIII, 27–36.Google Scholar
Papadakis, J. A. & Efstratiou, M. A. (1980). Isolation of salmonellae from fresh vegetables with the use of Rappaport–Vessiliadis magnesium chloride–malachite green enrichment medium. Hippocrates 5, 1–5.Google Scholar
Rappaport, F., Konforth, N. & Navon, B. (1956). A new enrichment medium for certain salmonellas. Journal of Clinical Pathology 9, 261–266.CrossRefGoogle Scholar
van Schothorst, M. & Renaud, A. M. (1983). Dynamics of salmonella isolation with modified llappaport's medium (R 10). Journal of Applied Bacteriology 54, 209–215.CrossRefGoogle Scholar
Skovgaard, N. & Nielsen, B. B. (1972). Salmonella in pigs and animal feeding stuffs in England and Wales and in Denmark. Journal of Hygiene 70, 127–140.CrossRefGoogle ScholarPubMed
Tongpim, S., Beumer, R. R., Tamminga, S. K. & Kampelmacher, E. H. (1984). Comparison of modified Rappaport's medium (RV) and Muller–Knufmann medium (MK-ISO) for the isolation of salmonella from meat products. International Journal of Food Microbiology 1. 33–42.CrossRefGoogle Scholar
Vassiliadis, P., Kalandidi, A., Xerouchaki, A., Palandiou, E. & Papadakis, J. (1976). Au suject du pH du milieu de Rappaport. Achives de l'Institute Pasteur Hellénique XXII, 43–49.Google Scholar
Vassiliadis, P., Trichopoulos, D., Kalapothaki, V. & Séké, Ch. (1981). Isolation of Salmonella with the use of 100 ml of the R10 modification of Rappaport's enrichment medium. Journal of Hygiene 87, 35–41.CrossRefGoogle ScholarPubMed
Vassiliadis, P. (1983). The Rappaport–Vassiliadis (RV) enrichment medium for the isolation of salmonellas: an overview. Journal of Applied Bacteriology 54, 69–76.CrossRefGoogle ScholarPubMed