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The use of a sandwich ELISA for the detection of staphylococcal enterotoxin A in foods from outbreaks of food poisoning

Published online by Cambridge University Press:  19 October 2009

Antonnette A. Wieneke  and
R. J. Gilbert
Antonnette A. Wieneke
Affiliation:
Food Hygiene Laboratory, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT
R. J. Gilbert
Affiliation:
Food Hygiene Laboratory, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT
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Summary

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Foods from outbreaks of food poisoning were examined for the presence of staphylococcal enterotoxin A (SEA) by a sandwich ELISA using microtitre trays as the solid phase and SEA antibodies raised in sheep.

The presence of SEA was confirmed by neutralization tests. The toxin was detected in 12 of 15 foods from separate outbreaks of staphylococcal food poisoning; all 15 foods contained a strain of Staphylococcus aureus which produced SEA. For most foods a simple extraction procedure without a concentration step was sufficient to detect the toxin. The method was semi-quantitative and recoveries of SEA added to control foods varied from 30 to 80%. The foods from outbreaks contained between 1 and 10 μg of SEA/100 g. SEA was not found in foods from 21 outbreaks in which an SEA-producing strain of Staph. aureus was not isolated.

Type
Research Article
Information
Journal of Hygiene , Volume 95 , Issue 1 , August 1985 , pp. 131 - 138
Copyright
Copyright © Cambridge University Press 1985

References

REFERENCES

Bergdoll, M. S. (1979). Stnphylococcal intoxications. In Food-Borne Infeciions and Intoxications, 2nd ed. (ed. Riemnnn, H. and Bryan, F. L.), pp. 443–494. New York: Academic Press.Google Scholar
Bradstreet, C. M. P., Dighero, M. W., Gilbert, R. J. & Wieneke, A. A. (1977). Raising antibodies to staphylococcal enterotoxins A and B. Journal of Applied Bacteriology 42, 117–122.CrossRefGoogle ScholarPubMed
Büning-Pfaue, H., Timmermans, P. & Notermans, S. (1981). Einfache Methode für den Nachweis von Staphylokokken-Enterotoxin-B in Vanillepudding mittels ELISA – Test. Zeitschrift für Lebensmittel-Untersuchung und -Forschung 173, 351–355.CrossRefGoogle Scholar
Casman, E. P. (1967). Staphylococcal food poisoning. Health Laboratory Science 4, 199–206.Google ScholarPubMed
Crowle, A. J. (1958). A simplified micro double-diffusion agar precipitin technique. Journal of Liboraiory and Clinical Medicine 52, 784–787.Google ScholarPubMed
de Saxe, M., Coe, A. W. & Wieneke, A. A. (1982). The use of phage typing in the investigation of food poisoning caused by Staphylococcus aureus enterotoxins. In Isolation and Identification Methods for Food Poisoning Organisms. Society for Applied Bacteriology Technical Series No. 17 (ed. Corry, J. E. L., Roberts, D. and Skinner, F. A.) pp. 173–197. London: Academic Press.Google Scholar
Duermeyer, W. (1980). Application of ELISA for the Diagnosis and Epidemiology of Hepatitis A, pp. 85–86. (Thesis, University of Utrecht). Amsterdam: Rodopi.Google Scholar
Fey, H., Pfister, H. & Rüego, O. (1984). Comparative evaluation of different enzyme-linked immunosorbent assay systems for the detection of staphylococcal enterotoxins A, B, C and D. Journal of Clinical Microbiology 19, 34–38.CrossRefGoogle Scholar
Freed, R. C., Evenson, M. L., Reiser, R. F. & Bergdoll, M. S. (1982). Enzyme-linked immuriosorbent assay for detection of staphylococcal enterotoxins in foods. Applied and Environmental Microbiology 44, 1349–1355.CrossRefGoogle ScholarPubMed
Gilbert, R. J. & Wieneke, A. A. (1973). Staphylococcal food poisoning with special reference to the detection of enterotoxin in food. In The Microbiological Safety of Food (ed. Hobbs, B. C. and Christian, J. H. B.), pp. 273–285. London: Academic Press.Google Scholar
Gilbert, R. J., Wieneke, A. A., Lanser, J. & Šimkovičová, M. (1972). Serological detection of enterotoxin in foods implicated in staphylococcal food poisoning. Journal of Hygiene 70, 755–762.Google ScholarPubMed
Igarashi, H., Shingaki, M., Fujikawa, H., Ushioda, H. & Terayama, T. (1984). Detection of staphylococcal enterotoxins in food poisoning outbreaks by reversed passive latex agglutination. In Proceedings of 5th International Symposium on Staphylococci and Staphylococcal Infections. Warsaw.Google Scholar
Kauffman, P. E. (1980). Enzyme immunoassay for staphylococcal enterotoxin A. Journal of the Association of Official Analytical Chemists 63, 1138–1143.Google ScholarPubMed
Kuo, J. K. S. & Silverman, G. J. (1980). Application of enzyme-linked immunosorbent assay for detection of staphylococcal enterotoxins in food. Journal of Food Protection 43, 404–407.CrossRefGoogle ScholarPubMed
Notermans, S., Timmermans, P. & Nagel, J. (1982). Interaction of staphylococcal protein A in enzyme-linked immunosorbent assays for detecting staphylococcal antigens. Journal of Immunological Methods 55, 35–41.CrossRefGoogle ScholarPubMed
Notermans, S., Boot, R., Tips, P. D. & De Nooy, M. P. (1983). Extraction of staphylococcal enterotoxins (SE) from minced meat and subsequent detection of SE with enzyme-linked immunosorbent assay (ELISA). Journal of Food Protection 46, 238–241.CrossRefGoogle ScholarPubMed
Reiser, R., Conaway, D. & Bergdoll, M. S. (1974). Detection of staphylococcal enterotoxin in foods. Applied Microbiology 27, 83–85.CrossRefGoogle ScholarPubMed
Saunders, G. C. & Bartlett, M. L. (1977). Double-antibody solid-phase enzyme immunoassay for the detection of staphylococcal enterotoxin A. Applied and Environmental Microbiology 34, 518–522.CrossRefGoogle ScholarPubMed
Šimkovičová, M. & Gilbert, R. J. (1971). Serological detection of enterotoxin from foodpoisoning strains of Staphylococcus aureus. Journal of Medical Microbiology 4, 19–30.CrossRefGoogle ScholarPubMed
Simon, E. & Terplan, G. (1977). Nachweis von Staphylokokken Enterotoxin B mittels ELISA-Test. Zenlralblatt für Veterinärmedizin Reihe B 24, 842–844.CrossRefGoogle ScholarPubMed
Stiffler-Rosenberg, G. & Fey, H. (1978). Simple assay for staphylococcal enterotoxins A, B and C: modification of enzyme-linked immunosorbent assay. Journal of Clinical Microbiology 8, 473–479.CrossRefGoogle Scholar