Hostname: page-component-5c6d5d7d68-thh2z Total loading time: 0 Render date: 2024-08-08T14:26:49.087Z Has data issue: false hasContentIssue false

The use of alkalinity and incubation at 9 °C for improved recovery of Yersinia spp. from faeces

Published online by Cambridge University Press:  15 May 2009

Melody H. Greenwood
Affiliation:
Public Health Laboratory, Poole General Hospital, Poole, BH15 2JB
W. L. Hooper
Affiliation:
Public Health Laboratory, Poole General Hospital, Poole, BH15 2JB
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Recovery of Yersinia enterocolitica and related strains from faecal samples enriched in 1% buffered peptone water (pH 7·2) and incubated at 4 °C for 17–21 days was compared with recovery from 1% peptone water buffered to pH 8·0 and incubation over the temperature range 4–26°C. Best recovery was obtained by use of the alkaline medium incubated at 9°C. Greatest recovery was obtained after incubation for 10–14 days, but most strains (> 75%) were recovered after 1 week.

Type
Special Article
Copyright
Copyright © Cambridge University Press 1988

References

REFERENCES

Aulisio, C. C. G., Mehlman, I. J. & Sanders, A. C. (1980). Alkali method for rapid recovery of Yersinia enterocolitica and Yersinia pseudotuberculosis from foods. Applied and Environmental Microbiology 39, 135140.CrossRefGoogle ScholarPubMed
Greenwood, M. H. & Hooper, W. L. (1985). Yersinia species in food and related environments. Food Microbiology 2, 263269.CrossRefGoogle Scholar
Greenwood, M. H. & Hooper, W. L. (1987). Human carriage of Yersinia spp. Journal of Medical Microbiology 23, 345348.CrossRefGoogle ScholarPubMed
Lewis, A. M. & Chattopadhyay, B. (1986). Faecal carriage rate of Yersinia species. Journal of Hygiene 97, 281287.CrossRefGoogle ScholarPubMed
Ratnam, S., Looi, C. L. & Patel, T. R. (1983). Lack of efficacy of alkali treatment for isolation of Yersinia enterocolitica from faeces. Journal of Clinical Microbiology 18, 10921097.CrossRefGoogle Scholar
Schiemann, D. A. & Olson, S. A. (1984). Antagonism by gram-negative bacteria to growth of Yersinia enterocolitica in mixed cultures. Applied and Environmental Microbiology 48, 539544.CrossRefGoogle ScholarPubMed
Weissfeld, A. S. & Sonnenwirth, A. C. (1982). Rapid isolation of Yersinia spp. from faeces. Journal of Clinical Microbiology 15, 508510.CrossRefGoogle Scholar