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Use of single radial immunodiffusion test for serological studies in volunteers inoculated with live attenuated influenza virus

Published online by Cambridge University Press:  15 May 2009

M. E. Molyneux ,
A. S. Beare ,
K. Callow  and
G. C. Schild
M. E. Molyneux
Affiliation:
Clinical Research Centre, Harrow, Middlesex, and Common Cold Unit, Salisbury, Wilts.
A. S. Beare
Affiliation:
Clinical Research Centre, Harrow, Middlesex, and Common Cold Unit, Salisbury, Wilts.
K. Callow
Affiliation:
Clinical Research Centre, Harrow, Middlesex, and Common Cold Unit, Salisbury, Wilts.
G. C. Schild
Affiliation:
National Institute for Medical Research, Mill Hill, NW 7 1AA
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Summary

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Pre- and post-vaccination serum samples from 278 volunteers, who were given live influenza vaccines, were tested by haemagglutination inhibition (HI) and single radial immunodiffusion tests (SRDT) for antibody to influenza A/Hong Kong/1/68 (H3N2) virus envelope antigens. Those with high antibody titres detected in both tests were less frequently infected, and 85% of the 159 infected showed rises by HI and 70% by SRDT. Similarly, 69 pairs were tested for antibody to Hong Kong (N2) neuraminidase by neuraminidase inhibition test (NI) and by SRD tests. Those with high titres in both tests resisted infection and those who were infected showed a rise in antibody detected both by NI and SRD tests. In general, SRDT was less sensitive than HI and NI in detecting antibody and antibody rises, but in some volunteers it did detect antibody rises which were not detected by conventional tests. Because of its simplicity and speed it appeared to be of use in evaluating such vaccines.

Type
Research Article
Information
Journal of Hygiene , Volume 73 , Issue 2 , October 1974 , pp. 287 - 294
Copyright
Copyright © Cambridge University Press 1974

References

REFERENCES

Aymard-Henry, M., Coleman, M. T., Dowdle, W. R., Laver, W. G., Schild, G. C. & Webster, R. G. (1973). Influenzavirus neuraminidase-inhibition test procedures. Bulletin of the World Health Organization 48, 199–202.Google ScholarPubMed
Beare, A. S., Bynoe, M. L. & Tyrrell, D. A. J. (1968). Investigation into the attenuation of influenza viruses by serial passage. British Medical Journal iv, 482–4.CrossRefGoogle Scholar
Hirst, G. K. (1942). The quantitative determination of influenza virus and antibodies by means of red cell agglutination. Journal of Experimental Medicine 75, 49–64.CrossRefGoogle ScholarPubMed
Kilbourne, E. D. (1969). Future influenza vaccines and the use of genetic recombinants. Bulletin of the World Health Organization 41, 643–5.Google ScholarPubMed
Mancini, G., Carbonara, A. O. & Heremans, J. F. (1965). Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry 2, 235–54.CrossRefGoogle ScholarPubMed
Schild, G. C., Berryman, I. L., Pereira, H. G. & Henry-Aymard, M. (1973). Quantitative single radial diffusion tests for immunological studies with influenza virus. Symposium Series Immunobiological Standardization 20, 39–46.Google Scholar
Schild, G. C., Henry-Aymard, M. & Pereira, H. G. (1972). A quantitative single radial diffusion test for immunological studies with influenza virus. Journal of General Virology 16, 231–6.CrossRefGoogle ScholarPubMed
Tyrrell, D. A. J. (1963). The use of volunteers. American Review of Respiratory Diseases 88, 128–34.Google ScholarPubMed
Tyrrell, D. A. J., Peto, M. & King, N. (1967). Serological studies on infections by respiratory viruses of the inhabitants of Tristan da Cunha. Journal of Hygiene 65, 327–41.Google Scholar
Vergani, C., Stabilini, R. & Agostini, A. (1967). Quantitative determination of serum immunoglobulins by single radial immunodiffusion on cellulose acetate. Immunochemistry 4, 233–7.CrossRefGoogle ScholarPubMed
Webster, R. G. & Laver, W. G. (1967). Preparation and properties of antibody directed specifically against the neuraminidase of influenza virus. Journal of Immunology 99, 49–55.Google Scholar