Hostname: page-component-848d4c4894-nmvwc Total loading time: 0 Render date: 2024-06-27T10:13:53.790Z Has data issue: false hasContentIssue false
  • English
  • Français

Vero cytotoxin-producing Escherichia coli, particularly serogroup O 157, associated with human infections in the United Kingdom: 1989–91

Published online by Cambridge University Press:  15 May 2009

A. Thomas ,
H. Chart ,
T. Cheasty ,
H. R. Smith ,
J. A. Frost  and
B. Rowe
A. Thomas
Affiliation:
Laboratory of Enteric Pathogens, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT
H. Chart
Affiliation:
Laboratory of Enteric Pathogens, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT
T. Cheasty
Affiliation:
Laboratory of Enteric Pathogens, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT
H. R. Smith
Affiliation:
Laboratory of Enteric Pathogens, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT
J. A. Frost
Affiliation:
Laboratory of Enteric Pathogens, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT
B. Rowe
Affiliation:
Laboratory of Enteric Pathogens, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

This survey reports the results of investigations performed by the Laboratory of Enteric Pathogens (LEP), to identify evidence of human infection with Vero cytotoxin-producing Escherichia coli (VTEC) in the UK during the period 1989–91. Bacterial isolates, faecal specimens and serum samples were received from patients suffering from diarrhoea, bloody diarrhoea and haemolytic uraemic syndrome. Using serotyping, Vero cytotoxin gene probing and an ELISA for serum antibodies to E. coli 0 157, evidence of infection was detected in 232, 428 and 615 individuals in 1989. 1990 and 1991 respectively. Of these individuals, 15% were reported as having HUS. Vero cytotoxin-producing E. coli O 157 was the most frequently encountered serogroup. with isolations from a total of 1092 individuals over the 3-year period. The incidence of VTEC infection increased from 0·41/ 100000 in 1989 to 1· 07/100000 in 1991. The area with the highest rate of infection in each year was Scotland, increasing from 1–37/100000 in 1989 to 3·97/ 100000 in 1991.

Type
Research Article
Information
Epidemiology & Infection , Volume 110 , Issue 3 , June 1993 , pp. 591 - 600
Copyright
Copyright © Cambridge University Press 1993

References

REFERENCES

1.Riley, LW. Remis, RS, Helgerson, SD. et al. Haemorrhagic colitis associated with a rare Escherichia coli serotype. New Eng J Med 1983; 308: 681–5.Google Scholar
2.Milford, DV. Taylor, CM. Guttridge, B, Hall, S, Rowe, B. Kleanthous, H. Haemolytic uraemic syndromes in the British Isles 1985–1988: association with Verocytotoxin producingEscherichia coli. Part 1: clinical and epidemiological aspects. Arch Dis Child 1990; 65: 716–21.CrossRefGoogle Scholar
3.Kleanthous, H. Smith, HR. Scotland, SM. et al. Haemolytic uraemic syndromes in the British Isles. 1985–1988: association with Verocytotoxin producing Escherichia coli. Part 2: Microbiological aspects. Arch Dis Child 1990; 65: 722–7.CrossRefGoogle Scholar
4.Smith, HR. Scotland, SM. Vero cytotoxin-producing strains of Escherichia coli. J Med Microbiol 1988: 26: 77–85.Google ScholarPubMed
5.Karmali, MA. Steele, BT. Petric, M. Lim, C. Sporadic cases of haemolytic-uraemic syndrome associated with faecal cytotoxin and cytotoxin producing Escherichia coli in stools. Lancet 1983: i: 619–20.Google Scholar
6.Taylor, CM. White, RHR. Winterborn, MH. Rowe, B. Haemolytic-uraemic syndrome: clinical experience of an outbreak in the West Midlands. BMJ 1986; 292: 1513–6.CrossRefGoogle ScholarPubMed
7.Morgan, GM. Newman, C. Palmer, SR, et al. A community outbreak of haemorrhagic colitis due to verotoxin producing Escherichia coli O 157: H7. Epidemiol Infect 1988; 101: 83–91.CrossRefGoogle ScholarPubMed
8.Salmon, RL, Farrell, ID, Hutchison, JGP, et al. A christening party outbreak of haemorrhagic colitis and haemolytic uraemic syndrome associated with Escherichia coli 0 157: H7. Epidemiol Infect 1989; 103: 249–54.Google Scholar
9.Scotland, SM, Rowe, B, Smith, HR, Willshaw, GA, Gross, RJ. Vero cytotoxin-producing strains of Escherichia coli from children with haemolytic uraemic syndrome and their detection by specific DNA probes. J Med Microbiol 1989; 25: 237–53.Google Scholar
10.Smith, HR, Rowe, B, Gross, RJ, Fry, NK, Scotland, SM. Haemorrhagic colitis and Vero-cytotoxin producing Escherichia coli in England and Wales. Lancet 1987; i: 1062–5.CrossRefGoogle Scholar
11.Marsh, J, MacLeod, AF, Hanson, MF, Emmanuel, FXS, Frost, JA, Thomas, A. A restaurant associated outbreak of E. coli O 157 infection. J Publ Health Med 1992; 14: 78–83.Google Scholar
12.Frost, JA, Smith, HR, Willshaw, GA, Scotland, SM, Gross, RJ, Rowe, B. Phage-typing of Verocytotoxin (VT) producing Escherichia coli 0 157 isolated in the United Kingdom. Epidemiol Infect 1989; 103: 73–81.CrossRefGoogle Scholar
13.Chart, H, Smith, HR, Scotland, SM, Rowe, B, Milford, DV, Taylor, CM. Serological identification of Escherichia coli 0 157: H7 infection in haemolytic uraemic syndrome. Lancet 1991; 337: 138–40.CrossRefGoogle Scholar
14.Cowan, ST, Steel, KJ. Manual for the identification of medical bacteria. Cambridge: Cambridge University Press, 1970.Google Scholar
15.Gross, RJ, Rowe, B. Serotyping of Escherichia coli. In: The virulence of Escherichia coli. Reviews and Methods, Sussman M, ed. London: Academic Press; 1985: 345–63.Google Scholar
16.Maniatis, T, Fritsch, EF, Sambrook, J. Molecular cloning: A laboratory manual. New York: Cold Spring Harbour Laboratory, 1982.Google Scholar
17.Thomas, A. Smith, HR, Willshaw, GA, Rowe, B. Non-radioactivity labelled polynucleotide and oligonucleotide DNA probes, for selectively detecting Escherichia coli strains producing Vero cytotoxins VT1, VT2 and VT2 variant. Mol Cell Prob 1991; 5: 129–35.CrossRefGoogle Scholar
18.Willshaw, GA, Smith, HR, Scotland, SM, Field, AM. Rowe, B. Heterogeneity of Escherichia coli phages encoding Vero cytotoxins: comparison of clones sequences determining VT1 and VT2 and development of specific gene probes. J Gen Microbiol 1987: 133: 1309–17.Google ScholarPubMed
19.Frost, JA. Cheasty, T. Thomas, A. Rowe, B. Phage typing of Vero cytotoxin-producing Escherichia coli 0 157 isolated in the United Kingdom: 1989–91. Epidemiol Infect 1993: 110: 469–475.CrossRefGoogle Scholar
20.Dev, VJ. Main, M. Gould, IM. Waterborne outbreak of Escherichia coli O 157. Lancet 1991: 337: 1412.CrossRefGoogle Scholar
21. Anonymous. Diarrhoea outbreak in old peoples' home. Comm Dis Env Health Scot Week Rep 1990; No. 90/25: 4.Google Scholar
22. Anonymous. E. coli O 157 infection. Comm Dis Env Health Scot Week Rep 1990: No. 90/42: 5.Google Scholar
23. Anonymous. Haemorrhagic colitis: Escherichia coli O 157. PHLS Comm Dis Rep 1991; 1, No. 6: 1.Google Scholar
24. Anonymous. Verotoxin producing Escherichia coli O 157: phage type 49. PHLS Comm Dis Rep 1991; 1, No. 47: 1.Google Scholar
25.Bisset, JG. Brown, MI. Bimson, G. et al. An outbreak of haemorrhagic colitis due to E. coli O 157 in 2 residential homes for the elderly in the Borders. Comm Dis Env Health Scot Week Rep 1992; 26. No. 92/19: 5–6.Google Scholar
26.Graham, L, Ramsay, C. A community outbreak of E. coli O 157. Comm Dis Env Health Week Rep 1991; 25, No. 91/43: 3–4.Google Scholar
27.Smith, HR. Cheasty, T. Robert, D. Thomas, A, Rowe, B. Examination of retain chickens and sausages in Britain for Vero cytotoxin-producing Escherichia coli. App Env Microbiol 1991: 57: 2091–3.CrossRefGoogle Scholar
28.Karmali, MA. Infection by Verocytotoxin-producing Escherichia coli. Clin Microbiol Revs 1989; 2: 15–38.Google ScholarPubMed
29.Synge, BA. Hopkins, GF. Verotoxigenic Escherichia coli O 157 in Scottish calves. Vet Rec 1992; 130: 583.CrossRefGoogle Scholar
30.Chapman, PA, Siddons, CA. Wright, DJ, Norman, P. Fox, J, Crick, E. Cattle as a source of Verotoxigenic Escherichia coli O 157. Vet Rec 1992; 131: 323–4.Google Scholar