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DNA methylation during antheridial filament development and spermiogenesis in Chara vulgaris (Charophyceae) analysed by in situ nick-translation driven by methylation-sensitive restriction enzymes

Published online by Cambridge University Press:  01 August 1997

MARIA J. OLSZEWSKA ,
DOROTA GERNAND ,
MIROSŁAW GODLEWSKI  and
AGNIESZKA KUNACHOWICZ
MARIA J. OLSZEWSKA
Affiliation:
Department of Plant Cytology and Cytochemistry, University of Łódź, Banacha, 12/16, 90-237 Łódź, Poland
DOROTA GERNAND
Affiliation:
Department of Plant Cytology and Cytochemistry, University of Łódź, Banacha, 12/16, 90-237 Łódź, Poland
MIROSŁAW GODLEWSKI
Affiliation:
Department of Plant Cytology and Cytochemistry, University of Łódź, Banacha, 12/16, 90-237 Łódź, Poland
AGNIESZKA KUNACHOWICZ
Affiliation:
Department of Plant Cytology and Cytochemistry, University of Łódź, Banacha, 12/16, 90-237 Łódź, Poland
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Abstract

DNA of Chara vulgaris thalli contains target sequences for the methylation-sensitive restriction enzymes, Sau3A, HpaII and MspI. These enzymes were used with the in situ nick-translation radioactive technique to evaluate by grain counting in autoradiograms changes in cytosine methylation during antheridial filament development and spermiogenesis. In successive generations of dividing antheridial filaments the level of DNA methylation gradually increases, then decreases slightly in the early stages of spermiogenesis. Spermatozoids display the highest level of DNA methylation.

Keywords

antheridial filamentsChara vulgarisin situ nick-translationmethylation-sensitive restriction enzymes
Type
Research Article
Information
European Journal of Phycology , Volume 32 , Issue 3 , August 1997 , pp. 287 - 291
Copyright
© 1997 British Phycological Society

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