People have learned in all situation take refuge from God and ask Him to help them and sharing with him their needs, desires and interests to get calm. The aim of this study was to examine the effects of worship level on the mental health of female students at the Islamic Azad University, Dezfoul Branch, Iran. ‘The statistical society of this study was all female students, Islamic Azad University of Dezfoul. The study sample included 120 female students that selected randomly in different parts of University. Descriptive and inferential statistical frameworks were applied to analyze and interpret the data. Tools for this research contain two researcher-designed questionnaires (assessment worship questionnaire) including 36 items with Likert 5 and MMPI questionnaire (questionnaire measuring psychological characteristics) included 71 questions. The research method was descriptive-field, and with questionnaire examining research factors. The study variables were the amount of worship (independent) and psychological characteristics (dependent). After selecting research participants randomly in the classroom, library, mosque, students’ canteen) and other places in universities, subjects satisfactory completion of the questionnaires. The results indicated a significantly negative relationship between the worship and hypochondria and depression. There was a significantly negative relationship between the worship depression and anxiety of the questionnaires. No significant relationship was found between worship and K index as well as average education of the questionnaires. The results of this study clarify that the prevention and treatment of mental disorders can catch our attention regarding the role of religion in human happiness and mental health.