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GENOTYPIC DIFFERENCES IN CARDINAL TEMPERATURES FOR IN VITRO POLLEN GERMINATION AND POLLEN TUBE GROWTH OF COCONUT HYBRIDS

Published online by Cambridge University Press:  25 July 2017

C. S. RANASINGHE ,
M. D. P. KUMARATHUNGE  and
K. G. S. KIRIWANDENIYA
C. S. RANASINGHE*
Affiliation:
Plant Physiology Division, Coconut Research Institute, Lunuwila 61150, Sri Lanka
M. D. P. KUMARATHUNGE
Affiliation:
Plant Physiology Division, Coconut Research Institute, Lunuwila 61150, Sri Lanka
K. G. S. KIRIWANDENIYA
Affiliation:
Plant Physiology Division, Coconut Research Institute, Lunuwila 61150, Sri Lanka
*
†Corresponding author. Email: sanathanie_ranasinghe@yahoo.com
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Summary

Successful fruit set in coconut depends on several reproductive processes including pollen germination and pollen tube growth. High temperature (˃33 °C) during flowering reduces fruit set in coconut. Therefore, identification and development of coconut varieties or hybrids with high reproductive heat tolerance will benefit the coconut industry in view of the climate changes. This experiment was conducted to quantify the response of pollen germination and pollen tube growth of seven coconut hybrids to increasing temperature from 16 to 38 °C. A Principal Component Analysis (PCA) was carried out to classify coconut hybrids on the basis of their temperature tolerances to pollen germination. Pollen germination and pollen tube length of the hybrids ranged from 56 to 78% and 242 to 772 µm, respectively. A modified bilinear model best described the response to temperature of pollen germination and pollen tube length. Cardinal temperatures (Tmin, Topt and Tmax) of pollen germination and pollen tube length varied among the seven hybrids. PCA identified Tmax for pollen germination and Topt for pollen tube length as the most important parameters in describing varietal tolerance to high temperature. PCA also identified SLGD × Sri Lanka Tall and Sri Lanka Brown Dwarf × Sri Lanka Tall as the most tolerant hybrids to high temperature stress and Sri Lanka Tall × Sri Lanka Tall and Sri Lanka Green Dwarf × San Ramon as less tolerant ones based on cardinal temperatures for pollen germination and pollen tube length. Tmax for pollen germination of the most tolerant and less tolerant hybrids were 41.9 and 39.5 °C, respectively. Topt for pollen tube length in the most tolerant and less tolerant hybrids were 29.5 and 26.0 °C, respectively.

Type
Research Article
Information
Experimental Agriculture , Volume 54 , Issue 5 , October 2018 , pp. 731 - 743
Copyright
Copyright © Cambridge University Press 2017 

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References

REFERENCES

Acar, I., Uzun, M., Atli, H. S. and Eti, S. (2010). Analysis of pollen germination performance of pistachio hybrids and their male parents. Journal of Food, Agriculture and Environment 8 (2):796–800.Google Scholar
Amarasinghe, K. G. A. P. K., Ranasinghe, C. S., Abeysinghe, D. C. and Perera, A. A. F. L. K. (2014). Effects of heat and drought stress on the development of reproductive Organs of different coconut (Cocos nucifera L.) cultivars under controlled pollination conditions in Sri Lanka. 2nd International Conference on advances in Plant Sciences (ICAPS 2014), November 18–22, Kuching, Malaysia, 60.Google Scholar
Burke, J. J., Velten, J. and Oliver, M. J. (2004). In vitro analysis of cotton pollen germination. Agronomy Journal 96:359–368.Google Scholar
Clement, C., Chavant, L., Burrus, M. and Audran, J. C. (1994). Anther starch variations in Lilium during pollen development. Sexual Plant Reproduction 7:347–356.Google Scholar
Djanaguiraman, M., Vara Prasad, P. V., Murugan, M., Perumal, R. and Reddy, U. K. (2014). Physiological differences among sorghum (Sorghum bicolor L. Moench) genotypes under high temperature stress. Environmental and Experimental Botany 100:43–54.Google Scholar
Firon, N., Shaked, R., Peet, M., Pharr, D. M., Zamski, E., Rosenfeld, K., Althan, L. and Pressman, E. (2006). Pollen grains of heat tolerant tomato cultivars retain higher carbohydrate concentration under heat stress condition. Scientia Horticulture 109:212–217.Google Scholar
Gajanayake, B., Trader, B. W., Reddy, R. and Harkess, R. L. (2011). Screening ornamental pepper cultivars for temperature tolerance using pollen and physiological parameters. Horti Science 46 (6):878–884.Google Scholar
Intergovernmental Panel on Climate Change (2007). Climate Change 2007, Impacts, adaptation and Vulnerability, Working Group II contribution to the Fourth Assessment Report of the IPCC, http://www.ipcc.ch/.Google Scholar
Kakani, V. G., Prasad, P. V. V., Craufurd, P. Q. and Wheeler, T. R. (2002). Response of in vitro pollen germination and pollen tube growth of groundnut (Arachis hypogaea L.) genotypes to temperature. Plant, Cell and Environment 25:1651–1661.Google Scholar
Kakani, V. G., Reddy, K. R., Koti, S., Wallace, T. P., Prasad, P. V. V., Reddy, V. R. and Zhao, D. (2005). Difference in in vitro pollen germination and pollen tube growth of cotton cultivars in response to high temperature. Annals of Botany 96:59–67.Google Scholar
Liyanage, D. V. (1950). Sex life of the coconut palm. Ceylon Coconut Quarterly 11 (2):33–35.Google Scholar
Liyanage, D. V. (1954). Controlled pollination of coconut palms. Ceylon Coconut Quarterly 5 (3):135–139.Google Scholar
Nadeeshani, S. M. A. O., Ranasinghe, C. S. and Warnasooriya, W. M. R. S. K. (2014). Temperature and rainfall variability on carbohydrate content of anthers and pollen germination in coconut. 6th Annual Research Symposium 2014, Rajarata University of Sri Lanka, 83.Google Scholar
Punyawardane, B. V. R. (2008). Evolution of climatic zones in Sri Lanka. In Agro-climatological Zones and Rainfall Pattern in Sri Lanka (in Sinhalese), 44–113. (Ed Punyawardane, B. V. R.). Sri Lanka: Department of Agriculture.Google Scholar
Rajagopal, V. and Kasturi Bai, K. V. (1999). Water relations and screening for drought tolerance. In Advances in Plant Physiology and Biochemistry of Coconut Palm, 56–70 (Eds Rajagopal, V. and Ramadasan, A.). Jakarta: Asian and Pacific Coconut Community.Google Scholar
Ranasinghe, C. S, Silva, L. R. S. and Premasiri, R. D. N. (2015). Major determinants of fruit set and yield fluctuation in coconut (Cocos nucifera L.). Journal of National Science Foundation 43 (3):253–264.Google Scholar
Ranasinghe, C. S., Premasiri, R. D. N. and Pradeep, A. P. C. (2014). Vulnerability of coconut based agroforestry systems in Sri Lanka to climate change. In Abstracts of the 3rd World Congress on Agroforestry: Trees for life: Accelerating the Impact of Agroforestry, 10–14 February, New Delhi, India, 287 (Eds Wachira, M. A., Rabar, B. and Magaju, C.). Nairobi: World Agroforestry Centre.Google Scholar
Ranasinghe, C. S., Waidyarathna, K. P., Pradeep, I. A. P. C. and Meneripitiya, M. S. K. (2010). Approach to screen coconut varieties for high temperature tolerance by in-vitro pollen germination. Cocos 19 (1):1–12.Google Scholar
Reddy, K. R. and Kakani, V. G. (2007). Screening Capsicum species of different origin for high temperature tolerance by in vitro pollen germination and pollen tube length. Scientia Horticulturae 112:130–135.Google Scholar
Shivanna, K. P. and Sawhney, V. K. (1997). Pollen Biotechnology for Crop Production and Improvement. Cambridge, UK: Cambridge University Press.Google Scholar
Snidder, J. L., Oosterhuis, D. M., Loka, D. A. and Kawakami, E. M. (2011). High temperature limits in vivo pollen tube growth rates by altering diurnal carbohydrate balance in field-grown Gossypium hirsutum pistils. Journal of Plant Physiology 168:1168–1175.Google Scholar
Sunilkumar, K., Mathur, R. K., Sparjanbabu, D. S. and Reddy, , A. G. K. (2013). Pollen viability and vigour in interspecific hybrids of oil palm. Journal of Plantation Crops 41 (1):91–94.Google Scholar
Thomas, R. J., Nair, R. V., Mathews, C., Ajithkumar, R., Sasikala, M. and Nampoothiri, C. K. (2012). Studies on fruit set in coconut upon artificial pollination in various cross combinations. Indian Journal of Horticulture 69 (1):7–12.Google Scholar