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Molecular characterization of sweet cherry genetic resources in Giresun, Turkey

Published online by Cambridge University Press:  24 January 2011

Taki Demir ,
Leyla Demirsoy ,
Hüsnü Demirsoy ,
Yıldız Aka Kaçar ,
Muharrem Yılmaz  and
Idris Macit
Taki Demir*
Affiliation:
Sakarya Univ., Vocat. Sch. Geyve, 54700, Geyve, Sakarya, Turkey,
Leyla Demirsoy
Affiliation:
Ondokuz Mayıs Univ., Fac. Agric., Dep. Hortic., Samsun, Turkey
Hüsnü Demirsoy
Affiliation:
Ondokuz Mayıs Univ., Fac. Agric., Dep. Hortic., Samsun, Turkey
Yıldız Aka Kaçar
Affiliation:
Cukurova Univ., Fac. Agric., Dep. Hortic., Adana 01330, Turkey
Muharrem Yılmaz
Affiliation:
Cukurova Univ., Fac. Agric., Dep. Hortic., Adana 01330, Turkey
Idris Macit
Affiliation:
Karadeniz Agric. Res. Inst., Samsun, Turkey
*
∗ Correspondence and reprints
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Abstract

Introduction. Turkey potentially has a very rich source of sweet (Prunus avium) and sour (P. cerasus) cherries. P. avium is apparently native to some parts of Northern Turkey, where Giresun is located. Identification of the sweet cherry cultivars produced in Turkey will help in choosing appropriate cultivars and aid in the preservation of natural resources required for breeding studies. The most conventional method of cultivar identification is based on the assessment of morphological characteristics. However, this method is insufficient to distinguish closely related cultivars. The aims of our study were to determine the molecular profile of sweet cherry accessions grown in Giresun, Turkey, and to determine their genetic relationships. Materials and methods. In our study, we identified 44 sweet cherry accessions grown in Giresun by using genetic markers (SSR, Simple Sequence Repeat), and we determined the genetic relationships among the sweet cherry genotypes. For DNA isolation, we collected young leaves sampled on a single plant per accession, then amplification of microsatellite loci was performed. In total, ten SSR primer pairs, previously isolated from peach and sweet cherry, were used. Genetic similarity values were calculated. A cluster analysis was performed to generate a dendrogram. Results and discussion. Of the ten primers tested, six primer pairs did not result in suitable amplification products with the 44 accessions studied. The remaining four polymorphic SSR primer pairs produced 33 alleles with an average of 8.25 putative alleles per locus, ranging from 7 to 11. Depending on the accessions, similarity ratios ranged from 0.32 to 0.98, with a mean value of 0.64. In conclusion, the results obtained demonstrate a high level of polymorphism among sweet cherry genotypes from a single province in Turkey.

Keywords

TurkeyPrunus aviumgenetic resourcesidentificationmicrosatellitesgenetic markersgenetic distanceTurquiePrunus aviumressource génétiqueidentificationmicrosatellitemarqueur génétiquedistance génétiqueTurquíaPrunus aviumrecursos genéticosidentificaciónmicrosatélitesmarcadores genéticosdistancia genética
Type
Original article
Information
Fruits , Volume 66 , Issue 1 , January 2011 , pp. 53 - 62
Copyright
© 2011 Cirad/EDP Sciences

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