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Analysis of lines of mice selected for fat content: 3. Flux through the de novo lipid synthesis pathway

Published online by Cambridge University Press:  14 April 2009

Emmanuel A. Asante ,
William G. Hill  and
Grahame Bulfield
Emmanuel A. Asante
Affiliation:
Cellular and Molecular Biology Department, AFRC Institute of Animal Physiology and Genetics, Edinburgh Research Station, Roslin, Midlothian EH25 9PS
William G. Hill
Affiliation:
Institute of Cell, Animal and Population Biology, University of Edinburgh, West Mains Road, Edinburgh EH9 3JT*
Grahame Bulfield
Affiliation:
Cellular and Molecular Biology Department, AFRC Institute of Animal Physiology and Genetics, Edinburgh Research Station, Roslin, Midlothian EH25 9PS

Summary

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The flux through the de novo fatty acid synthesis pathway was estimated in lines of mice which differed substantially in fat content following 26 generations of selection at 10 weeks of age. Previous estimates of lipogenic enzyme activities had indicated an increase in the capacity for lipogenesis in the Fat compared to the Lean line. Therefore the in vivo flux in lipogenesis was measured in both liver and gonadal fat pad (GFP) tissues of males at 5 and 10 weeks of age, using the rat of incorporation of 3H from 3H2O and 14C from acetate and citra te into total lipids. AT both ages and in both tissues the Fat line had a higher flux, about 20% increase in the liver and up to three-fold increase (range 1·2- to 3·4-fold) in the GFP. We conclude that direct selection for fatness in mice has resulted in metabolic changes in the ratio of de novo fatty acid synthesis, and that the changes are largely detectable before 10 weeks, the age of selection.

Type
Research Article
Information
Genetics Research , Volume 58 , Issue 2 , October 1991 , pp. 123 - 127
Copyright
Copyright © Cambridge University Press 1991

References

Asante, E. A., Hill, W. G. & Bulfield, G. (1989). Analysis of lines of mice selected for fat content. 1. Correlated responses in the activities of NADPH-generating enzymes. Genetical Research 54, 155–160.Google Scholar
Baker, N., Learn, B. D. & Bruckdorfer, K. R. (1978). Reevaluation of lipogenesis from dietary glucose carbon in liver and carcass of mice. Journal of Lipid Research 19, 879–893.CrossRefGoogle ScholarPubMed
Bulfield, G., Issacson, J. H. & Middleton, R. J. (1988). Biochemical correlates of selection for weight-for-age in chickens: twenty-fold higher muscle ornithine decarboxylase levels in modern broilers. Theoretical and Applied Genetics 75, 432–437.Google Scholar
Folch, J., Lee, M. & Sloane-Stanley, G. H. (1957). A simple method for the isolation and purification of total lipides from animal tissues. Journal of Biological Chemistry 226, 497–509.CrossRefGoogle ScholarPubMed
Gandemer, G., Pascal, G. & Durand, G. (1985). Comparative changes in the in vivo fatty acid synthesis in liver and adipose tissues during the post-weaning growth of male rats. Comparative Biochemistry and Physiology 82B, 581–586.Google Scholar
Goodridge, A. G. (1968). Metabolism of glucose U-14C in vitro in adipose tissue from embryonic and growing chicks. American Journal of Physiology 214, 897–901.CrossRefGoogle ScholarPubMed
Hastings, I. M. (1989). Genetical and biochemical analyses of growth. PhD. Thesis, University of Edinburgh.Google Scholar
Hastings, I. M. & Hill, W. G. (1989). A note on the effect of different selection criteria on carcass composition in mice. Animal Production 48, 229–233.CrossRefGoogle Scholar
Hastings, I. M. & Hill, W. G. (1990). Analysis of lines of mice selected for fat content. 2. Correlated responses in the activities of enzymes involved in lipogenesis. Genetical Research 55, 55–61.CrossRefGoogle ScholarPubMed
Hems, D. A., Rath, E. A. & Terence, R. V. (1975). Fatty acid synthesis in liver and adipose tissue of normal and genetically obese (ob/ob) mice during the 24-hour cycle. Biochemical Journal 150, 167–173.Google Scholar
Jansen, G. R., Hutchison, C. F. & Zanetti, M. E. (1966). Studies on lipogenesis in vivo: Effect of dietary fat or starvation on conversion of [14C]glucose into fat and turnover of newly synthesised fat. Biochemical Journal, 99, 323–332.Google Scholar
Leveille, G. A., Romsos, D. R., Yeh, Y. Y. & O'Hea, E. K. (1975). Lipid biosynthesis in the chick. A consideration of site of synthesis, influence of diet and possible regulatory mechanisms. Poultry Science 54, 1075–1093.Google Scholar
Rath, E. A. & Thenen, S. W. (1980). Influence of age and genetic background on in vivo fatty acid synthesis in obese (ob/ob) mice. Biochimica et Biophysica Acta 618, 18–27.CrossRefGoogle ScholarPubMed
Rogers, P. & Webb, G. P. (1980). Estimation of body fat in normal and obese mice. British Journal of Nutrition 43, 83–86.Google Scholar
Sharp, G. L., Hill, W. G. & Robertson, A. (1984). Effects of selection on growth, body composition and food intake in mice 1. Responses in selected traits. Genetical Research 43, 75–92.Google Scholar