Hostname: page-component-77c89778f8-gq7q9 Total loading time: 0 Render date: 2024-07-17T10:38:05.212Z Has data issue: false hasContentIssue false
  • English
  • Français

Chromosome rearrangements resulting in increased aneuploid production in Sordaria brevicollis

Published online by Cambridge University Press:  14 April 2009

D. J. Bond  and
S. J. Broxholme
D. J. Bond
Affiliation:
Department of Genetics, King's Buildings, University of Edinburgh, Edinburgh EH9 3JN, Scotland
S. J. Broxholme
Affiliation:
Department of Genetics, King's Buildings, University of Edinburgh, Edinburgh EH9 3JN, Scotland

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Two spontaneously arising chromosome rearrangements were isolated in Sordaria brevicollis from one perithecium. These were detected through increased production of black spores in intercrosses of complementing buff spore colour mutants. One was a reciprocal translocation between linkage groups I and II; the other a reciprocal translocation between linkage groups II and VI. In the former case the translocation resulted in frequent non-disjunction generating black spores which were either tertiary or interchange disomics. The frequency of premature centromere division was also increased. In the case of the translocation involving linkage groups II and VI the black spores were formed as a result of adjacent-1 segregation and were probably duplication/deficiency products.

In both rearrangements the breakpoint in linkage group II was, as far as could be judged, in an identical place. This and the fact that they were isolated from a single fruiting body, suggests that the chromosome breakage event arose as a potential lesion, which replicated before the potential break was either restituted, to restore a normal chromosome, or opened, to form the rearrangements.

Type
Research Article
Information
Genetics Research , Volume 42 , Issue 3 , December 1983 , pp. 297 - 310
Copyright
Copyright © Cambridge University Press 1983

References

REFERENCES

Alfi, O. S., Chang, R. & Azen, S. P. (1980). Evidence for genetic control of nondisjunction in Man. American Journal of Human Genetics 32, 477–483.Google ScholarPubMed
Auerbach, C. (1951). Problems in chemical mutagenesis. Cold Spring Harbor Symposium, Quantitative Biology 16, 199–213.CrossRefGoogle ScholarPubMed
Auerbach, C. & Moser, H. (1953). Analysis of the mutagenic action of formaldehyde food. II. The mutagenic potentialities of the treatment. Zeitschrift für Abstammungs u. Vererbungslehre 85, 547–563.Google ScholarPubMed
Bond, D. J. (1976). A system for the study of meiotic non-disjunction using Sordaria brevicollis. Mutation Research 37, 213–220.CrossRefGoogle Scholar
Bond, D. J. (1983). Systems for detecting meiotic aneuploidy in Sordaria and Neurospora. In ‘Handbook of Mutagenicity Test Procedures’, 2nd ed. (ed. Kilbey, B. J., Legator, M., Nicholls, W. and Ramel, C.). Holland Elsevier.Google Scholar
Bond, D. J. & McMillan, L. (1979 a). Meiotic Aneuploidy: Its origins and induction following chemical treatment in Sordaria brevicollis. Environmental Health Perspectives 31, 67–74.CrossRefGoogle ScholarPubMed
Bond, D. J. & McMillan, L. (1979 b). The effect of p–fluorophenylalanine on the frequency of aneuploid meiotic products in Sordaria brevicollis. Mutation Research 60, 221–224.CrossRefGoogle ScholarPubMed
Burnham, C. R. (1949). Chromosome segregation in maize translocations in relation to crossing over in interstitial segments. Proceedings of the National Academy of Science 35, 349–356.CrossRefGoogle ScholarPubMed
Delange, A. M. & Griffiths, A. J. F. (1980). Meiosis in Neurospora crassa. II. Genetic and cytological characterization of three meiotic mutants. Genetics 96, 379–398.CrossRefGoogle ScholarPubMed
Fulton, A. M. & Bond, D. J. (1983). An investigation into the origins of meiotic aneuploidy using ascus analysis. Genetical Research 41, 165–179.CrossRefGoogle Scholar
Griffiths, A. J. F. & Delange, A. M. (1977). p–fluorophenylalanine increases non-disjunction in a Neurospora test system. Mutation Research 46, 345–354.CrossRefGoogle Scholar
Hecht, F. (1982). Unexpected encounters in cytogenetics: Repeated abortions and parental sex chromosome mosaicism may indicate risk of nondisjunction. American Journal of Human Genetics 34, 514–16.Google ScholarPubMed
Herman, R. K., Kari, C. K. & Hartman, P. S. (1982). Dominant X-chromosome nondisjunction mutants of Caenorhabditis elegans. Genetics 102, 379–400.CrossRefGoogle ScholarPubMed
Lindenbaum, R. M. & Bobrow, M. (1975). Reciprocal translocations in Man. 3:1 meiotic disjunction resulting in 47- or 45-chromosome offspring. Journal of Medical Genetics 12, 29–43.CrossRefGoogle ScholarPubMed
Perkins, D. D. & Barry, E. G. (1977). The cytogenetics of Neurospora. Advances in Genetics 19, 133–285.CrossRefGoogle ScholarPubMed
Slizynska, H. (1957). Cytological analysis of formaldehyde induced chromosomal changes in Drosophila melanogaster. Proceedings of the Royal Society, Edinburgh 66, 288–304.Google Scholar
Vogel, H. J. (1956). A convenient growth medium for Neurospora (Medium N). Microbial Genetics Bulletin 13, 42–43.Google Scholar