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A gamma ray-induced non-excitable membrane mutant in Paramecium caudatum: a behavioral and genetic analysis

Published online by Cambridge University Press:  14 April 2009

Mihoko Takahashi ,
Nobuyuki Haga ,
Todd Hennessey ,
Robert D. Hinrichsen  and
Ritsuo Hara
Mihoko Takahashi
Affiliation:
Institute of Biological Sciences, University of Tsukuba, Sakura-mura, Ibaraki 305, Japan
Nobuyuki Haga
Affiliation:
Laboratory of Molecular Biology, University of Wisconsin-Madison, Madison, Wisconsin 53706, U.S.A.
Todd Hennessey
Affiliation:
Laboratory of Molecular Biology, University of Wisconsin-Madison, Madison, Wisconsin 53706, U.S.A.
Robert D. Hinrichsen
Affiliation:
Laboratory of Molecular Biology, University of Wisconsin-Madison, Madison, Wisconsin 53706, U.S.A.
Ritsuo Hara
Affiliation:
Institute of Biological Sciences, University of Tsukuba, Sakura-mura, Ibaraki 305, Japan

Summary

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A new CNR (caudatum non-reversal) mutant of Paramecium caudatum was isolated after gamma ray mutagenesis. This CNR lacks not only the transient inward Ca2+ current but also the sustained Ca2+ current. It was shown to complement the three known CNR mutants of P. caudatum (cnrA, cnrB and cnrC) by crossbreeding analyses. Thus, this new mutant belongs to a 4th CNR locus, designated cnrD. The defect of cnrD can be partially rescued by microinjection of cytoplasm from any of the three CNR mutants or the three Pawns (pwA, pwB and pwC) in P. tetraurelia. Since the three CNR genes have been shown to be different from the three Pawn genes by cytoplasmic complementation test (Haga et al. 1983), this result suggests that cnrD is the 7th non-excitable mutant in Paramecium. Thus, there are at least seven genes controlling Ca2+ channel function in Paramecium.

Type
Research Article
Information
Genetics Research , Volume 46 , Issue 1 , August 1985 , pp. 1 - 10
Copyright
Copyright © Cambridge University Press 1985

References

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